Protein methods

place or not. Also, synthesis could determine "how much" transcription is necessary. Positive control is the method that can help start the transcription through protein binding and is often regulated by things such as glucose. On the other hand, negative control can help stop transcription when necessary. This type of control also involves the binding of a protein, but it is a repressing protein as opposed to an activator. Although transcription is different in prokaryotes and Eukaryotes, there…

The study is aimed at the comparison of homologous protein BLOCKs using different diversity parameters (MDRs, DHPs and MCRs etc) that are formulated using positional frequencies of observed hetero-pairs and homo-pairs of BLOCKs. APBEST, written in AWK programming language, extracts these BLOCK specific parameters. How efficient is the program? Are these parameters correlate with already existing literature reports? To have resolution of these questions, we have implemented the program first on…

solutions are prepared by dissolving the compound in DMSO. Antimicrobial activity was tested by the disc-diffusion method under standard conditions using Mueller-Hinton Agar (MHA) and the antibiotics used as listed below, Table 1.As control MTCC K. pneumoniae 7407, MTCC S. aureus 3160 and ATCC E.coliJ53. Antimicrobial disc diffusion susceptibility AST was done by Disc-Diffusion method (Qureshi AH et al., 2004) for all the derivatives at the concentration 1mg/ml to find out the zone of…

endonuclease CRISPR associated protein 9 (Cas9). These complexes allow the enzyme to be shuttled to the target DNA to perform a cut on one or both strands of the foreign DNA in either of the enzyme’s two active sites. Recently, scientists have repurposed this bacterial defense mechanism as a genome editing utility to cut, shuttle, or bind desired molecules anywhere within DNA as custom RNA harnesses the multi-tool Cas-9 enzyme (Gupta & Musunuru, 2014). Unlike other methods of genome engineering…

overlooked source, cohesion fatigue can induce chromosome instability. The authors focused on the cohesion complex in this study since in a previous study they identified that a certain protein, Ska3, was important in the maintaining of chromosome cohesion2. This implies that there is a possibility of other proteins that can destabilize chromosome cohesion, thus creating chromosome instability. 2. Cohesion fatigue is a phenomenon named by the authors, in which during induced metaphase delay,…

The deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) within organisms can be methylated while proteins can be either methylated or acetylated. The methylation of DNA and RNA is used for alternative splicing used for production of different mRNA molecules resulting in numerous new proteins. One of the molecules believed to perform the methylation is N6A or N6 methyladenosine. Methylation is the most observed modification to mRNA molecules. Methylation of both DNA and RNA ensures…

Bio Lab- Denaturing Proteins Period B- Ms. Dann October 8, 2014 Nick D’Ambrosio, Matt Hermann, Carly Pruitt Title: Electrophoresis and Protein Weight Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to…

Hobert (2000) believes, that the patterns of these LIM-homeodomain proteins is evident from studies about Drosophila to C. elegans. LIM proteins have their own transcription factors and cytoskeletal elements. Structurally, a LIM domain has cysteines with 2 zinc fingers. These LIM homeodomain (LIM-HD) proteins regulate the formation of cell line cells and influence differentiation. (Dawid et al., 1995) To control these proteins, it is suspected that they all have a DNA-binding specificity due to…

The folding mechanism of knotted/slipknotted protein has become a challenging question over the last two decades.(10, 89, 90, 95, 99, 100, 173-175) Understanding how those proteins fold would be a great proceeding of the field of protein folding. Recent advances on protein folding shed light on this question using both experimental and computational methods. .(10, 89, 90, 95, 99, 100, 173-175) Most experimental studies on the folding of knotted protein have been limited to bulk experiments.(84,…

disease. Site-specific incorporation of genetic code reprogramming have demonstrated powerful techniques for investigation proteins and as a tool for biologically-mediated organic chemical synthesis of peptides. The replacement of amino acids with the non-canonical amino acid allows scientist to understand the role of a specific amino acid in the polypeptide molecules. This new method of site-specificity…