Protein methods

Epigenetics, a contemporary and often misunderstood topic in the field of biology, was first defined in the early 1940s as “the branch of biology which studies the causal interactions between genes and their products with bring the phenotype into being” (Dupont 2009). Since then, as understanding of the human genome has developed and grown, a complex array of errors and dysfunctions in the processes of gene regulation, expression, and replication have been shown to cause, or correlate strongly…

Title: How Temperature Affects Protein Detection In Eggs Whites Introduction: Research Objective and Hypothesis The objective of this experiment is to gain a better understanding on how temperature affects how much protein is detected. Understanding how temperature affects the detection of protein can be used to more accurately measure the amount of protein in foods and supplements needed for life. This knowledge can benefit nutritionists and the general population. Hypothesis: If the egg…

Analysis of Consumer Products with Unknown Protein Concentrations Using Spectrophotometry Abstract Consumer products typically give detailed information about their ingredients and nutrients. However, the concentration of a specific nutrient may not always be clear from just the label information alone, and in some cases the product labels may not be accurate. Thus, it may be hard to compare the concentration level of a certain nutrient, such as protein, amongst different products. In this…

GENE REGULATION: Gene regulation is the method of limiting the genes in the cell’s DNA, which are expressed. It is utilize to form a functional manufacture for an example protein. Various cells in a multi cellular organism might express very difficult group of genes. Although, they consist the similar DNA. Human having over ten thousands of genes in their genome. Cells are expressed the all genes. Even an organism as simple as bacterium must carefully regulate gene expression. Ensuring that the…

Immunofluorescence methods are restricted to capture a snap shot of live-cells; therefore, I plan to image FLCN lysosomal dissociation in cells that were designed by crispr methods to fluorescently tag FLCN with GFP and LAMP2 with RFP. Then with live time-lapse imaging using spinning-disk confocal to track this FLCN dissociation in HEK293T cells seeded…

Bacterial Transformation Ian Nall BIO 181 | Section #73116 | Wednesday 1:30-4:15pm Abstract This experiment was designed to test the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed in either…

armenica seeds was extracted according to method outlined by Han et al. [19] with slight modification. The seeds were soaked in distilled water for 24 hr at room temperature, washed with distilled water, grounded with chilled ethyl acetate, air dried and stored at 4 °C. The dried powder material…

Sarah Diaz Dr. Basu Bio 572 My Favorite gene One of my favorite genes that I have had the pleasure of working with is known as PROMININ-1, or PROM-1. One of the reasons why it exists as my favorite gene, is because the research lab that I am interning at studies this gene and its role in a very well known and unfortunate disease, biliary atresia. It also is one my favorites due to the fact that it has a lot of potential applications in studying other diseases or further research. These…

The resulting dysfunctional GLUT-1 protein causes inadequate amounts of glucose to be transported to the brain and results in neurologic disorders such as childhood intractable epilepsy (1). Although introduced nearly a century ago, the ketogenic diet has gained increasing popularity within the last decade to treat epileptic seizures, especially when minimal or no improvement is seen with anti-epileptic drugs (AEDs) (2). The diet is a very successful method of attenuating seizures and…

isomerisation. Introduction Bacteriorhodopsin is a globular protein, (Henderson, 1975) which acts as a light driven proton- pump located in the native purple membrane of Halobacterium halobium. It was first discovered by Stoechenius and his lab in 1979 (Stoeckenius, 1979). Although Henderson and Unwin had already made a three-dimensional map of…