Protein methods

treat it in its early stages. With that being said, there have been no real advances in the early detection of pancreatic cancer. Most current methods of detection, such as biopsies, are too invasive and costly. However, recent research…

Objective: In-silico assessment analysis of Protein database entries of the Pf. lactate dehydrogenase (PfLDH) enzyme, considered as an important target for designing of anti-malarial drugs. Methods: PDB structures of the enzyme Pf. lactate dehydrogenase were downloaded from Protein Data Bank. These were subjected to ANOLEA energy assessment analysis and Swiss model was used to analyse Ramachandran plot. Results: The energy assessment analysis and analysis of Ramachandran plot were carried out…

building block of protein to life. For example, hemoglobin, the cells in our bone marrow, churn out a hundred trillion per second of hemoglobin, which is a red protein responsible for transporting oxygen in the blood of the vertebrates. Life consists of genomes, which contain all the information to build and support an organism. Rewriting genomes can eventually affect and change…

glutaraldehyde, but showed a significant decrease in pore diameter and total pore volume as the compound was covalently bonded to the KIT-6 surface. Besides, the pore diameter of KIT-6 obtained was considered sufficient to accommodate the medium size protein molecule. The FESEM image gave supporting evidence for the mesoporous silica KIT-6 with a gyroid structure in complement to the SAXS analysis, signifying the KIT-6 with Ia3d symmetry and the 3D-interconnecting pore network. Besides that, the…

Proteins Introduction Many products including powders, drinks, and foods are marketed as having more protein in them than the average consumption. But is that statement actually true? Proteins are a type of amino acid. There are twenty known amino acids monomers. Monomers are molecules that can be bonded together to create something else known as a polymer. Every amino acid has to have three things, an amino group, a carboxyl group and a functional group that is special to itself. Something…

organic molecules it contains. These tests are referred to as the Benedict’s, Lugol’s, Biuret, and Sudan IV tests and are each used to identify an organic compound. For instance, Benedict’s checks for reducing sugars, Lugol’s for starch, Biuret for protein, and Sudan IV for lipids. When testing the unknown with each reagent, the substance only tested positive for reducing sugars. Based on the results of this experiment and what was known about the possible unknowns it could have been, it was…

extraction the proteins absorb light at 260nm and 280nm, especially the aromatic acids. 2. 250µg to nm, 250 x 1000 = 250,000ng. 3. 200ng/ml = 0.2µg/ml. 150µg/ml = 150,000µg/ml 250,000pg/µl = 250µg/ml 1mg/ml = 1000µg/ml ● 150,00µg/ml - HIGHEST CONCENTRATION OF DNA ● 1000µg/ml ● 250µg/ml ● 0.2µg/ml - LOWEST CONCENTRATION OF DNA 4. Sodium dodecyl sulphate is used as a detergent, which allows the cells to release DNA by disrupting the cell and nuclear membranes. It dissolves the…

of Nrf-2 would increase the antioxidant potential of macrophages exposed to hyperoxia by induction of antioxidant protein, HO-1, leading to a reduction in iROS and the subsequent prevention of HMGB1 release. Significant advances in this field will have the potential to provide clinical and therapeutic approaches for patients suffering from hyperoxia and VAP. MATERIALS AND METHODS Cell Line Procedure Murine macrophage RAW 264.7 cells were cultured in Dulbecco’s…

The purpose of this research is to understand how secondary structures of a protein, in this case sperm whale apomyoglobin, affect the denatured state of that protein. The scientific community has been working under the impression that once denatured by urea, a protein loses all of its secondary structure. In this research the effect that apomyoglobin’s history of secondary structure has on the denatured state of the protein will be determined through kinetics of the refolding reaction. The…

Column Chromatography is considered one of the main techniques of separation in addition to SDS-PAGE and gel electrophoresis. This method can be useful in both a small scale as in a laboratory experiment as well as in a larger scale at the industrial level. It is also applied in different fields ranging from biology and biochemistry to microbiology and medicine. Column chromatography is also referred to as gel filtration or size exclusion chromatography among many other terminologies and it is…