Molecular biology

Today, the 1953 discovery of the double helix, “the twisted-ladder structure” of DNA, by James Watson and Francis Crick, constitutes one of the greatest breakthroughs in the history of science. The discovery has given rise to the molecular biology of today and generated new insights into genetic coding upon which today’s multi-billion dollar biotechnology industry is founded. It laid the groundwork for other scientific achievements, including modern forensics and the mapping of the human…

in research related to biochemical because biochemistry has made it easy to understand the living processes, this made biochemistry more successful since last few decades of the 20th century. Now-a-days, studying and understanding the biological molecular processes that takes place within a living cell is greatly…

Literature Review: Green Fluorescent Protein and Histidine Tagged Proteins Histidine and green fluorescent protein (GFP) tagging are important in molecular biology because they allow for purification, tracking, and quantification of target proteins (Ferrari et al., 2004; Cho et al., 2011; Deponte, 2012). Histidine tagging provides a method for isolating and increasing the amount of target protein recovered from a biological organism or mixed sample of proteins (Masek et al., 2011; Singh and…

cells is a topic that is able to be assisted by Fluidgm technology, a biotech company that focuses on developing and manufacturing instruments, kits and reactants for single cell biology and high throughput screening. Fluidgm technologies as well as other biotech companies incorporate the central dogma of molecular biology (DNA-RNA-Proteins) into…

What is the education requirement for this career? A: The education requirement is based off of a four year degree plan in Biology, Chemistry, Genetics, Molecular genetics, Molecular biology, Forensic science, Physics, Criminalistics, and Biochemistry. Few of the undergraduate degrees individuals may pursue for forensic DNA analyst careers include Bachelor of Science in Biology, DNA Analysis Methods, Bachelor of Science in Biological Sciences, and more. Graduate programs often pursued by…

most common DNA amplification method in molecular biology, it was invented by Kary Mullis while working in Emeryville, California for Cetus Corporation, one of the first biotechnology companies. His invention won him a Nobel Prize in Chemistry in 1993. PCR has revolutionized the field of molecular biology as it has enabled researchers to perform experiments easily that previously had been unthinkable. Before the mid-1980s, when PCR was developed, molecular biologists had to use laborious and…

A Variable Number Tandem Repeat (VNTR) is a molecular motif that plays a considerable role in molecular genetics and specifically forensic analysis [1]. The goal of this experiment is to observe the tandem repeats of the VNTR (D1S80) locus through molecular lab techniques. Cheek cells were isolated from an individual and amplified via a polymerase chain reaction (PCR). Then, the amplified sample was separated through gel electrophoresis. This project entails the interpretation of fluorescing…

Introduction Information processing is crucial in all fields of science. In molecular biology, the central dogma, first devised by Francis Crick, is a classical backbone of living cells to essentially perform the processes from cell division to death through the DNA, RNA, and protein information ways. More specifically, the central dogma defines the transfer of sequence information during DNA replication, transcription into RNA, and translation into amino-acid chains forming proteins. It also…

knowledge from my graduate and undergraduate programs came in handy for my professional internship, directed studies and my current Student Assistant job at A&M. First semester’s Molecular Biology course revised most of the cell biology concepts that I learned from my undergraduate studies and gave insights into advanced cell biology. To complement our understanding, we were assigned to read high impact scientific journal articles as a daily class assignments. Having developed the analytical…

that is present in just about all living organisms as the top chromosomes. DNA Isolation is the development of purification of DNA from a sampling applying a mixture of physical and chemical methods. Isolation is a routine process in molecular biology or in molecular analyses. Materials: • Electrophoresis chamber • Gel casting trays • Sample combs • Ethidium bromide • Loading buffer • Electrophoresis buffer • Transilluminator • DNA • Cotton swabs • Distilled water • Tablespoon of salt • Soap…