DNA sequencing

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  • Essay On Dna Sequencing

    Introduction: The discovery of Deoxyribonucleic acid (DNA) as genetic material by Oswald Theodore Avery in 1944 paved the way for modern technology in the life sciences. Scientists James D. Watson and Francis Crick determined that this genetic material was composed for four bases in 1953, which lead to the central dogma of molecular biology (Liu, et al., 2009). To better understand what this chemical code means, Alan Maxam and Walter Gilbert developed the Maxam-Gilbert manual DNA sequencing method in 1976. This method uses chemical reactions to break glycoside bonds within the double stranded DNA, to create a single stranded DNA with a radioactive label on the 5’ end. The labeled substrate is then cleaved to create known endpoints and these fragments are run on a polyacrylamide gel using electrophoresis. The gel would then use…

    Words: 724 - Pages: 3
  • DNA Sequencing In Science And Technology

    DNA sequencing and information is useful in many fields of science and technology. DNA ideally can be used to identify specific organisms because most organisms don’t possess the same DNA.The use of DNA to try to identify specific organisms is called DNA fingerprinting. DNA fingerprinting involves creating a DNA fingerprint using gel electrophoresis, which is the process by which DNA is broken down into fragments and then sorted by positive electrodes (after negative electrodes repel them from…

    Words: 783 - Pages: 4
  • Sequencing Essay

    With the advancements made in technology daily and new scientific studies and explorations, the science community is always on the brink of something big. And the next big thing might be designer babies. Deeper and more profound exploration into cells and genetics have allowed for the possibility of designer babies to emerge. Before creating designer babies was even an idea, scientists were first working towards sequencing the human genome. By sequencing the human genome, scientists will be able…

    Words: 1108 - Pages: 5
  • KOH String Test Lab Report

    if it gram positive or gram negative you can do addition testing like a Catalase test, growth on Mannitol Salt Agar test or an Oxidase test. The EMB-lactose, PEA, and vancomycin containing agar plates are used to test media for biochemical and cellular classification. To confirm the amplification of the 16S rRNA gene of the bacteria you use the performing gel electrophoresis. The PCR DNA is used to obtain a DNA sequence and use the DNA sequencing software to edit and use the NCBI database to…

    Words: 1945 - Pages: 8
  • Recombinant Plasmid Case Study

    From these results, it cannot be determined if the recombinant plasmid was the desired one or not because no fragments appeared on the gel that allowed for identification of the fragments involved in the recombinant. The only lane that contained a band was lane B which contained the BamHI and HindIII digest of the pAMP plasmid. Due to the location of the single band, its molecular weight falls between 4000 and 5000 base pairs. Since the pAMP plasmid is known to be 4539 bps, it is likely that…

    Words: 1087 - Pages: 5
  • Essay On Gibberellins

    libraries and DNA microarrays. We also learnt how DNA analysis involves looking for the initiator sequence, promoter, enhancer, TATA box, CAT box and G-C rich region. We then headed to a library exhibition, as the Cambridge library as an institution was marking its 600th anniversary. They had an exhibition on heredity. A section of the exhibition was all about Darwin’s later works that was set up specially for us! Darwin did a lot of practical work during the latter stages of his career. He…

    Words: 1315 - Pages: 6
  • Dna Polymorphism Lab Report

    The experiments covered in this report are based on the topic of DNA polymorphisms, these according to Houseman (1995) are differences in the DNA that occur between individuals. The polymorphisms under investigation here are classified as VNTR’s (variable number tandem repeats) and RFLP’s (restriction fragment length polymorphism). The first experiment is concerned with DNA polymorphisms that are known as VNTR’s, sections of DNA which have repeated nucleotide base sections in which the repeats…

    Words: 829 - Pages: 4
  • Human DNA Fingerprinting

    According to http://www.encyclopedia.com, DNA which stands for deoxyribonucleic acid is used for human genetic makeup. It has different sequences of bases and exist in human body. The sequence of it nucleotides are A, T, G, C; or, adenine, thymine, guanine, and cytosine, respectively. A DNA fingerprinting, is a DNA pattern that has a unique sequence such that it can be distinguished from the DNA patterns of other individual. The two major uses for the information is for personal identification…

    Words: 1690 - Pages: 7
  • Alicia Horsfield: The Evolutionary Theory Of Evolution

    The more closely species are related the more closely their DNA sequences will overlap. Even very distantly related species will have some degree of DNA sequence overlap. Similar DNA species are closely related. DNA Sequencing is used in genetics lab to find missing pieces in DNA strands. An example of how DNA sequencing is how these animals are all related. Figure Two: Than, Ker. "What Is Darwin 's Theory of Evolution?" Live Science.com. Purch, 13 May 2015. Web. 20 Apr. 2016. Figure two…

    Words: 736 - Pages: 3
  • Difference Between Sanger And Next Generation Sequencing

    biology, is the Bioinformatics. The way it is doing it is by improvement and be able to sequencing DNA (genome). Bioinformatics can be describes as a science tool in which use computer technology to analyze, collect, “storage, and retrieval of genomic data” (American Heritage Dictionary). There are different ways to sequence the genome or DNA, store and evaluate the information obtained. Some of the methods are Sanger and Next Generation Sequencing, also known as hierarchical and whole-genome…

    Words: 790 - Pages: 4
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