DNA sequencing

Introduction: Bacteria are very diverse and are part of the prokaryotic domain. Some bacteria can be harmful, but others can also be beneficial in many ways. There are bacteria everywhere on Earth; places that you would think would have bacteria, like your gastrointestinal tract or our skin (Wenner, 2007). Although bacteria are small in size and are a simple cell organism, it is essential to life. There are different ways in how to distinguish and classify bacteria. One way is you can look…

gene therapy trial at the University of Pennsylvania due to lack of informed consent and eligibility criteria for participation. Human genome project is other issue that is of major concern to privacy breach. With the advanced technology in genome sequencing and analysis, we are now able to decipher human genome. However, this leads to problems like patient future health concerns. One his/her genome sequence was known, using current bio-informatics databases we can predict about any possible…

The experiments covered in this report are based on the topic of DNA polymorphisms, these according to Houseman (1995) are differences in the DNA that occur between individuals. The polymorphisms under investigation here are classified as VNTR’s (variable number tandem repeats) and RFLP’s (restriction fragment length polymorphism). The first experiment is concerned with DNA polymorphisms that are known as VNTR’s, sections of DNA which have repeated nucleotide base sections in which the repeats…

According to http://www.encyclopedia.com, DNA which stands for deoxyribonucleic acid is used for human genetic makeup. It has different sequences of bases and exist in human body. The sequence of it nucleotides are A, T, G, C; or, adenine, thymine, guanine, and cytosine, respectively. A DNA fingerprinting, is a DNA pattern that has a unique sequence such that it can be distinguished from the DNA patterns of other individual. The two major uses for the information is for personal identification…

comparison of nucleotide sequences changes to uncover the difference in the presence and absence of alu insertions, and the various alleles occurred by restriction enzyme digestion. In this lab, the presence of the alu alleles were investigated in the DNA of individual 1 and 2 to determine whether the alu elements can be used as genetic marker to identify race. The null hypothesis stated that alu elements could not be used as a genetic marker to identify race. The alternative hypothesis (H1)…

Upon completion of the PCR, the final product should be highly concentrated DNA corresponding to the gene of interest. We ran our gene of interest on gel electrophoresis to compare our gene of interest to a marker of known size to confirm the size of our genomic DNA. On the image of the gel (figure 1), the sample containing the DNA ran, but the sample containing the DNA ladder did not. As a result, an appropriate determination of the sizes cannot be made because there is no ladder to compare it…

this study used mitochondrial DNA sequence data in hopes of showing that A. geoffroyi and another spider monkey (Ateles fusciceps) form two separate monophyletic groups. The only other study to use molecular data for determining phylogeny in Mesoamerican spider monkeys was the Collins and…

What aspects of DNA’s structure contribute to the stability of the DNA molecule? The DNA’s double helix structure and the hydrogen bonding that bonds the bases contributes to the stability of the DNA molecule. 2. The DNA sequence is: 5’-ATTCGACC-3’. What will be the sequence of the complementary strand using this strand as the template? The complementary strand would be TAAGCTGG. 3. When the base composition of double-stranded DNA from a new species of bacteria was determined, 30 percent of the…

DNA Extraction A three millimeter square piece sample of unknown thawed tissue was selected using sterilized equipment. The A17 sample was placed in a steril in a 1.5mL Eppendorf tube. The cell membrane of the unknown meat was then broken down and the DNA was extracted by using the animal tissue extraction kit, Qiagen #69506. The final Eppendorf tube contained the DNA from A17 and was stored at -20 oC until gel electrophoresis. Gel Electrophoresis To determine the success of DNA extraction and…

In order to evaluate the efficiency of RAPD-PCR fingerprinting for Cajanus cajan L. cultivar, the DNA templates for 7 RAPD primers banding modality were used. The total number of magnified fragments in the Cajanus cajan L. cultivar value of each primer was represented in Table 2. Total of 18 bands were obtained for all primers. The size of the amplified bands was ranged between 1000 bp and 300 bp. Primers OP-A5, OP-B4, OP-B9, OP-C5 and OP-E15 showed at least three specific fragments. Primer…