DNA sequencing

November 25, 2014 DNA Virtually anything can be identified by DNA, also known as Deoxyribonucleic Acid. It is a method of identification of growing and living things. According to genome.gov, a DNA molecule consists of two strands that bind around one another to form a shape known as a double helix. Each strand of the double helix has a backbone made of interchanging sugar and phosphate groups. “DNA sequencing is a scientific technique used to define the exact arrangement of bases in a DNA…

Two colonies where then taken from this plate and placed into two tubes. These tubes where then taken and stored at a temperature of 37 degrees Celsius where it was able to grow overnight. After it was able to grow the plasmid DNA was isolated using Quick Lyse Minirep. The mixture was then mixed in order to resuspend the cells that had settled on the bottom and pipeted into a Quick Lyse Lysis. This tube was then Cetrifuged at 13,000 rpm and washed with a buffer. After being washed…

versions of the one common ancestor. The example is used for speciation is that birds, theropods, sauropods, snakes, and lizards all share a common ancestor that split from one missing link. To resume, common ancestry involves using fossils and DNA sequencing to prove that all organisms share…

The DNA quantitation is conducted to determine the amount of DNA is contained within a given biological sample. Biological samples are sent to the laboratories with the intention of developing DNA profile. It is important for the high quality development that the analyst first determines the quantity of DNA present in a sample. If there is too much DNA present, this can cause off-scale data in the final detection in a sample. Off-scale data can be littered with artifacts and extra peak…

process of altering an existing gene in the DNA and inserting it into cells in an organism to fix genetic disorders or diseases. In humans, gene therapy is the process in which some of the defect cells or the cells carrying the disease are removed from the body (eg. lung cells in a Cystic Fibrosis patient) in order to harvest their DNA. The base sequence of their DNA is then altered to remove the genetic disorder by deconstructing and reconstructing DNA through techniques such as PCR, ligation…

Chromatin Immunoprecipitation and Sequencing (ChIP-seq) of Heat Shock Factor (HSF) Temporal Binding Sites during the Critical Period of 8-12 Hr After Egg Lay (AEL) in Drosophila melanogaster Embryogenesis Background Heat Shock Response was discovered by the Italian scientist Ferrucio Ritossa, when he saw puffs in the Drosophila polytene chromosome on inducing them with heat. It was later discovered that this Heat Shock Response was regulated by a transcription factor called Heat Shock Factor…

mutations in patient’s gene and treatment would be done accordingly to target that specific mutation. The proliferation of bioinformatics and computational genomics plays a pivotal role in making this incentive effective. From extracting the raw data of DNA to understanding the genetic variations and clinical indication of these information, bioinformatics is leveraging these implications towards creating the personalized…

The doctors show that people can use lots of different ways to go wrong with personal DNA. Genome Sequenced can help people find out the mutations; However, most of the people think that Genome Sequenced are cheating them. For example, it may confuse doctors, scare patients and drive up costs. In the article, “YOU CAN GET YOUR WHOLE GENOME SEQUENCED. BUT SHOULD YOU? “(2017) MEGAN MOLTENI shows Genome Sequenced can help patients take care their body and almost no side effects. First of all,…

determination by slab gel electrophoresis. Forensic DNA analysis was introduced in the 1980s and became established with the discovery of polymorphic mini-satellites or variable number of tandem repeat (VNTR) loci which gave rise to the concept of genetic fingerprinting in forensic science (Gill, Jeffreys and Werrett, 1985). This changed emphasis from gene product…

Japan, France, and Germany (1). The overall aim of the Human Genome Project was to completely understand and map out every human gene, the genome (2). The other goals of the program were to determine the sequences of the chemical base pairs that create DNA, store the new understanding in databases, improve tools for this data analysis, and address social, ethical and legal implications of the project (1). Overall, the Human Genome Project has helped many…