Capillary electrophoresis

We still need to find out who this person was all the down to the bottom of the gel in DNA. To begin finding out who this person is we need to use DNA out of their bones and finger print samples. Also we will go through a missing persons report, that has occurred in the past week and try to match them up. Another investigation strategy we will use is, electrophoresis to analyze the pattern of bands that are left behind. After we took samples of DNA out of the bones we used gel electrophoresis to separate the DNA fragments according to their size. Then we stain the DNA with a binding dye so we can see the fragments as bands, that represent groups of same sized DNA fragments. We use the polymorphisms to find out who this person was; because every one has their own unique position and amount of these. We will use electrophoresis to separate and measure the length of these fragments and ultimately find the genotypes of that individual and find out who this person…

Ventilation and perfusion work together in the body to provide oxygen and dispose of carbon dioxide. Ventilation provides tissue and organs oxygen by the process of mechanical inspiration and exhalation of the lungs. This provides the alveoli within the lungs with oxygen, as well as the ability to expel waste products like carbon dioxide. Perfusion provides the tissues and organs with oxygen by the mechanism of diffusion between the pulmonary capillaries and alveoli. Ventilation Ventilation…

Diffusion across this pressure gradient is so effective that the capillary blood is fully oxygenated by the time it has passed through 1/3rd of the alveolar capillary network (Grossman & Porth, 2014). Ficks law of diffusion describes the relationship between factors that influence diffusion rates. It can be used to look at diffusion between the alveoli and this pulmonary capillary network. These factors include the partial pressure difference of gas (p1-p2), the thickness (T) and surface area…

Introduction: Langerhan’s cell histiocytosis is a malignancy caused by uncontrolled proliferation of the Langheran’s cell line with associated granulomatous deposition. The disease exists in a wide spectrum of clinical presentations ranging from localized skin manifestation to diffuse disease with neurological involvement. Typically, diabetes insipidus occurs when the hypothalamic-pituitary axis is affected by histiocytic infiltration. Langheran’s cell histiocytosis can be confirmed by…

DNA Extraction A three millimeter square piece sample of unknown thawed tissue was selected using sterilized equipment. The A17 sample was placed in a steril in a 1.5mL Eppendorf tube. The cell membrane of the unknown meat was then broken down and the DNA was extracted by using the animal tissue extraction kit, Qiagen #69506. The final Eppendorf tube contained the DNA from A17 and was stored at -20 oC until gel electrophoresis. Gel Electrophoresis To determine the success of DNA extraction and…

The main objective of the laboratory was to identity different species of honeybees based on their physical and genetic attributes. Due to the invasion of African honeybees in North America, hybridization has increased between African and European honeybees. The hypothesis is that the four unknown bees are hybridized with a European mother and an African father. The wing-lengths of European, African, and unknown bees were measured, and the mtDNA of eight different bees were subjected to gel…

mortars. The geopolymer binders with WTS and the additional RHA became the mortars more consistent and difficult to mix and mold, and the entrapped air remained in the mortars. Fig. 7 shows the capillary water absorption. The higher the WTS content in the mixture, the lower the capillary water…

DNA Gel Electrophoresis Electrophoresis is a process in which macromolecules are separated by utilizing their electrical charge and size. A technique for separating protein molecules of varying sizes in a mixture by moving them through a block of gel, as of agarose or polyacrylamide, by means of an electric field, with smaller molecules moving faster and therefore farther than larger ones. Gel electrophoresis of DNA is used in DNA profiling. When DNA is found at a crime scene, it's sent to a…

INTRODUCTION: What is DNA? DNA is a deoxyribonucleic acid, which is a self-replicating material that is present in just about all living organisms as the top chromosomes. DNA Isolation is the development of purification of DNA from a sampling applying a mixture of physical and chemical methods. Isolation is a routine process in molecular biology or in molecular analyses. Materials: • Electrophoresis chamber • Gel casting trays • Sample combs • Ethidium bromide • Loading buffer •…

DNA fingerprinting, also known as DNA profiling, is identifying an individual using there DNA. This is often used for identifying criminals, and parental testing. First, a sample must be provided, which can be: blood, semen, hair roots, or saliva. The cells from the sample are then split open, and DNA is separated from the rest of the cell. The DNA is then treated with specialized proteins (restriction enzymes),which separate the DNA into smaller parts. The fragments of DNA are then applied to a…