Abstract:
Genetic sequencing has brought about many scientific advancements, but techniques and approaches to sequencing vary, as do the goals of the sequencing. After reviewing first, second, and third-generation sequencing types, it would seem that both Sanger and Illumina-type sequencing would be most applicable to the data needed for phylogenetic systematics depending on mostly on cost and scale of research. Illumina, along with other sequencers such as those offered by 454 and nanopore, are well-suited for metagenomics and microbial studies.
Introduction
Since the discovery of the structure of DNA by Watson and Crick, DNA has become a tool by which scientists may study the genetic makeup of organisms, identifying relationships and characteristics …show more content…
Under this method, DNA is denatured into single-strand fragments that bind to microscopic beads, and are then amplified by an oil emulsion PCR (Huse et al 2007). The beads are then placed individually in the many wells of a Picotiterplate along with sulphurylase, luciferase, and dNTPs. These reagents serve to produce a light reaction in each well that is detected by a camera and recorded into a …show more content…
Despite this, Sanger sequencing methods continue to be used for smaller-scale (and cheaper) projects and can potentially verify the results of other sequencing methods. For example, if one were to conduct a phylogenetic study on a group of eukaryotic organisms such as birds or lizards, it is often that only a few loci out of an organism’s genome actually needs to be sequenced, perhaps less than five genes. Due to its lower cost and the lack of need for high-throughput, Sanger sequencing would be the more ideal choice. In the case of larger scale projects that would require the sequencing of multiple loci and multiple species, unless the collected data has already been sequenced, Sanger sequencing would be too time-consuming and
Genetic sequencing has brought about many scientific advancements, but techniques and approaches to sequencing vary, as do the goals of the sequencing. After reviewing first, second, and third-generation sequencing types, it would seem that both Sanger and Illumina-type sequencing would be most applicable to the data needed for phylogenetic systematics depending on mostly on cost and scale of research. Illumina, along with other sequencers such as those offered by 454 and nanopore, are well-suited for metagenomics and microbial studies.
Introduction
Since the discovery of the structure of DNA by Watson and Crick, DNA has become a tool by which scientists may study the genetic makeup of organisms, identifying relationships and characteristics …show more content…
Under this method, DNA is denatured into single-strand fragments that bind to microscopic beads, and are then amplified by an oil emulsion PCR (Huse et al 2007). The beads are then placed individually in the many wells of a Picotiterplate along with sulphurylase, luciferase, and dNTPs. These reagents serve to produce a light reaction in each well that is detected by a camera and recorded into a …show more content…
Despite this, Sanger sequencing methods continue to be used for smaller-scale (and cheaper) projects and can potentially verify the results of other sequencing methods. For example, if one were to conduct a phylogenetic study on a group of eukaryotic organisms such as birds or lizards, it is often that only a few loci out of an organism’s genome actually needs to be sequenced, perhaps less than five genes. Due to its lower cost and the lack of need for high-throughput, Sanger sequencing would be the more ideal choice. In the case of larger scale projects that would require the sequencing of multiple loci and multiple species, unless the collected data has already been sequenced, Sanger sequencing would be too time-consuming and