Gel Electrophoresis Lab Report

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Gel electrophoresis is a method used for separation and analysis of molecules such as DNA, RNA, and proteins, based on their sizes and polarity. DNA (deoxyribonucleic acid) is a molecule that carries most of our genetic information, and possesses a negative charge. During gel electrophoresis, DNA fragments can migrate through the gel also known as agarose when placed in a powerful electrical field. The rate at which the DNA fragments will move through the gel depends on their relative size. Horizontal gel slabs are commonly used on conducting gel electrophoresis. The agarose gel is poured onto a plastic plat forming wells, and then DNA samples are placed in small wells. When samples are added to their relative wells, gel and plastic plate are …show more content…
Our hypothesis that was formulated was trying to explain how a child with both parents as carriers has a risk of acquiring and having sickle cell anemia.
To text our hypothesis different methods and different materials were used to get the clear results. Materials that were used are as follows; agarose gel, micropipette and tips, DNA samples, fast stain, gel knife, light box, warm water, and lastly gloves. Prepared agarose gel was given; gel was held in a plastic bag to keep it from contamination, plastic bag was removed carefully and the gel was placed into the gel box. Aligning the wells at the negative end of the box was very important. With the gel being inside the box, running buffer was used to fill up the gel until the wells were covered with the buffer. Next step was to use the micropipette and provided tips to fill up each well with 35 uL of each sample in their separate wells. Everything was checked to make sure everything was properly done. Electrodes were hooked in their desired locations, power source was provided to the gel box, and timer was set for 15 minutes. Upon completion gel was properly stained, and the light box was used to visualize the gel for clear results. Main safety issue during this experiment was to be very careful with the gel, being careful with the use
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The hypothesis that was proposed was accepted based on the clear results that we gained after the experiment was finished. The hypothesis proposed if the child’s parents are both carriers, child will likely have sickle cell anemia. Both parents were carriers for sickle cell anemia (HbAHbS), which gave the child small chance of acquiring sickle cell anemia. The chance of acquiring sickle cell anemia was relatively low as compared being a carrier. The chance for the child to have sickle cell were ¼ only 25%, as opposed to being a carrier 2/4 50%, and being normal ¼ 25%. Chances for the child to have sickle cell were relatively small, but that did not exclude the fact there was a chance for him to acquire the mutated gene from both parents. Sickle cell anemia is autosomal disease which gets passed on from generation to generation, parents are most likely to pass their mutated genes to their offspring’s, in which same case was with their child. The child acquired the mutated gene from both parents, which led him/her to have sickle cells anemia. In any future experiments on sickle cell anemia, proper staining must be done to get the best results. Staining process must be more than once or twice to get the clear results with each sample being easily visible to the

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