Oral Microbiome 10: Reflection 3
One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped reduced the amounts of bacteria present in a person’s mouth (Cildir et al., 2009). This could mean that other types of food, maybe even food related to yogurt such as dairy, could help reduce the amount of bacteria present as well. In this lab we asked ourselves, as a group, how can we reduce the amount of S. mutans or …show more content…
To start these procedures a person will do a test using Colony PCR. This will be done by touching a toothpick to a selected colony and swirling with the matching 25 uL of PCR. Lastly, a person will visualize their DNA using gel electrophoresis. First, add 1.0 uL of 10X loading dye and 10uL PCR product and mix. Then Use a micropipette to load 10 uL of sample in the next open well, this will be adjacent to the preloaded DNA ladder done by the teaching assistant. Once all samples are loaded, close the box, and connect the cathodes and anodes. Run at 150 volts for forty to forty-five minutes, and stop when the bands have moved two thirds of the way down the gel. Next, turn off the power supply, remove the lid, and remove the gel. Then, the person will obtain an image of their gel using a UV transilluminator and a digital gel imaging system. Lactobacilli will have a 230 bp band, and S. mutans will have a 500 bp band. This will all to help determine the S. mutans and Lactobacilli present in the plated salvia during each data collection, including the
One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped reduced the amounts of bacteria present in a person’s mouth (Cildir et al., 2009). This could mean that other types of food, maybe even food related to yogurt such as dairy, could help reduce the amount of bacteria present as well. In this lab we asked ourselves, as a group, how can we reduce the amount of S. mutans or …show more content…
To start these procedures a person will do a test using Colony PCR. This will be done by touching a toothpick to a selected colony and swirling with the matching 25 uL of PCR. Lastly, a person will visualize their DNA using gel electrophoresis. First, add 1.0 uL of 10X loading dye and 10uL PCR product and mix. Then Use a micropipette to load 10 uL of sample in the next open well, this will be adjacent to the preloaded DNA ladder done by the teaching assistant. Once all samples are loaded, close the box, and connect the cathodes and anodes. Run at 150 volts for forty to forty-five minutes, and stop when the bands have moved two thirds of the way down the gel. Next, turn off the power supply, remove the lid, and remove the gel. Then, the person will obtain an image of their gel using a UV transilluminator and a digital gel imaging system. Lactobacilli will have a 230 bp band, and S. mutans will have a 500 bp band. This will all to help determine the S. mutans and Lactobacilli present in the plated salvia during each data collection, including the