Task 2 was to create a proposal to determine the quantitative characteristics that could control the light that is emitted from glow sticks. For task 3 the problem was to find one or more trends that could quantitatively control the amount of light emitted from the glow sticks. This investigation has value to companies or people that want to find a way to increase the brightness of glow sticks.
Experimental Design and Procedure
To control the amount of light emitted from the glow stick we decided to investigate with the variable, increasing alkalinity. Our goal with this variable was to measure the intensity of the glow stick stock when increasing the amount of Potassium Hydroxide in ethanol. To achieve this, …show more content…
Wipe 4mL vial with KimWipes and place vial into the vial block and test absorbency (click collect, wait for data to process, click stop to end data)
7. For the variable, alkalinity, fill 6 more 4mL vials with 1.5mL of the original glow stick solution using 1.0mL syringe, cap, and cover in foil.
8. One by one use micropipette to add potassium hydroxide in ethanol (install micropipette tip, spin plunger button to designated volume in the display window, press the plunger button to the second stop outside of solution and hold, place tip inside potassium hydroxide in ethanol solution, release plunger button, take tip out of potassium hydroxide in ethanol solution, place tip inside 4mL vial with glow stick solution, press the plunger button to the first stop, take tip out of glow stick solution and dispose of tip)
9. Cap vial, wipe with KimWipes and place vial block and test absorbency (click Collect, chose Store Last Run, wait for data to process, and click Stop to end data)
10. Record intensity of solution by finding the peak (maximum) of the graph into a table (intensity vs. amount of potassium hydroxide in ethanol solution)
11. Repeat steps 8-10 with each amount of potassium hydroxide in ethanol (1st vial with 50µm, 2nd with 75µm, 3rd with 100 µm, 4th with 125 µm, 5th with 150 µm, and 6th with 200