Abstract
The objective of this lab is to analyze DNA samples using gel electrophoresis and reaction enzymes and then comparing them to the DNA found at a “crime scene” to detect a match. After the experiment was complete the
Materials & Methods
Part 1
To begin this part of the experiment there needs to be a couple of reaction tubes labeled 1 through 4. First 10 microliters of reaction buffer to reaction tubes 1 and 2. You will then need to add 15 microliters of suspect 1’s DNA to both tubes (1 and 2). After that add 15 microliters of reaction enzyme 1 to reaction tube 1 and 15 microliters of reaction enzyme 2 to reaction tube 2. If done correctly there should be a final volume of both tubes should be 40 microliters. Repeat this process …show more content…
Tap each reaction tube gently to mix before putting them all into and incubator set at 37 degrees Celsius for 45 minutes. Place in a refrigerator after incubation is complete. Start casting and loading gel for electrophoresis while the reaction tubes are in the incubator.
Part 2
Measure out .4 for grams of Agarose to achieve a .8% gel concentration in a 50ml gel cast. Combine agarose and 50ml of the buffer solution into a flask, then place into microwave to heat until the agarose is dissolved. While the flask is cooling set up the gel box so that the gel tray fits securely into the box and the comb is placed in the correct place. The combs are there to form wells once the hot gel has cooled around the teeth of the combs. Add 2.5 microliters ethidium bromide; allows the DNA to be visible under UV light once the agarose solution has cooled significantly. Be …show more content…
The 2nd sample of DNA from the crime scene only matches the DNA from sample 2 in lane seven.
Discussion
Since the DNA in lane four and six match with the 1st sample of DNA from the crime scene that shows that some of the DNA fragments from the two suspects are similar. If only the first sample of DNA from the crime scene was used, both suspects would be considered as “guilty” or that they were both at the crime scene. However, the 2nd DNA sample from the crime scene only matched the DNA of suspect 2 (lane seven), meaning suspect one was not at the crime scene but suspect 2 was because there DNA matched both of the DNA fragments from the crime scene.
Although the idea DNA fingerprinting is commonly associated with crime scenes, its usage isn’t limited to one area. DNA fingerprinting can be used to determine whether two individuals are related. It can also be used within the medical to world for example if someone needed an organ, bone marrow transplant or even a blood transfusion DNA fingerprinting would be able to determine if that person is a
The objective of this lab is to analyze DNA samples using gel electrophoresis and reaction enzymes and then comparing them to the DNA found at a “crime scene” to detect a match. After the experiment was complete the
Materials & Methods
Part 1
To begin this part of the experiment there needs to be a couple of reaction tubes labeled 1 through 4. First 10 microliters of reaction buffer to reaction tubes 1 and 2. You will then need to add 15 microliters of suspect 1’s DNA to both tubes (1 and 2). After that add 15 microliters of reaction enzyme 1 to reaction tube 1 and 15 microliters of reaction enzyme 2 to reaction tube 2. If done correctly there should be a final volume of both tubes should be 40 microliters. Repeat this process …show more content…
Tap each reaction tube gently to mix before putting them all into and incubator set at 37 degrees Celsius for 45 minutes. Place in a refrigerator after incubation is complete. Start casting and loading gel for electrophoresis while the reaction tubes are in the incubator.
Part 2
Measure out .4 for grams of Agarose to achieve a .8% gel concentration in a 50ml gel cast. Combine agarose and 50ml of the buffer solution into a flask, then place into microwave to heat until the agarose is dissolved. While the flask is cooling set up the gel box so that the gel tray fits securely into the box and the comb is placed in the correct place. The combs are there to form wells once the hot gel has cooled around the teeth of the combs. Add 2.5 microliters ethidium bromide; allows the DNA to be visible under UV light once the agarose solution has cooled significantly. Be …show more content…
The 2nd sample of DNA from the crime scene only matches the DNA from sample 2 in lane seven.
Discussion
Since the DNA in lane four and six match with the 1st sample of DNA from the crime scene that shows that some of the DNA fragments from the two suspects are similar. If only the first sample of DNA from the crime scene was used, both suspects would be considered as “guilty” or that they were both at the crime scene. However, the 2nd DNA sample from the crime scene only matched the DNA of suspect 2 (lane seven), meaning suspect one was not at the crime scene but suspect 2 was because there DNA matched both of the DNA fragments from the crime scene.
Although the idea DNA fingerprinting is commonly associated with crime scenes, its usage isn’t limited to one area. DNA fingerprinting can be used to determine whether two individuals are related. It can also be used within the medical to world for example if someone needed an organ, bone marrow transplant or even a blood transfusion DNA fingerprinting would be able to determine if that person is a