Human DNA Fingerprinting

Improved Essays
Show More
According to http://www.encyclopedia.com, DNA which stands for deoxyribonucleic acid is used for human genetic makeup. It has different sequences of bases and exist in human body. The sequence of it nucleotides are A, T, G, C; or, adenine, thymine, guanine, and cytosine, respectively. A DNA fingerprinting, is a DNA pattern that has a unique sequence such that it can be distinguished from the DNA patterns of other individual. The two major uses for the information is for personal identification and for the determination of paternity. DNA can be analyzed from a variety of human samples including blood, semen, saliva, urine, hair, buccal (cheek cells), tissues, or bones. The polymerase chain reaction (PCR) is used to amplify the genomic DNA from a sample and electrophoresis is used to arrange the segment. …show more content…
Electrophoresis was then used to make analysis. During this lab activity, we were required to do human DNA fingerprinting which is a method used to identify individuals and to prove their relationships. Due to time and the length of this lab activity, the experiment was divided into part one: DNA Purification from Buccal Swabs, part two: PCR (polymerase chain reaction) amplification of the d1s80 VTNR locus from human genomic DNA and Part three: Analysis of PCR products using gel electrophoresis. We had a specific activity to carry out during each lab section.
PART ONE: DNA Purification from Buccal Swabs
The materials used for this lab section are listed below:
Materials
• Sterile

Related Documents

  • Improved Essays

    Blood Stained Tee Shirt Case Study Answers
    • 746 Words
    • 3 Pages

    Blood Stained Tee Shirt Case Study Answers

    Blood Stained Tee Shirt Collection of item: I would wear disposable latex gloves to packaged the blood stained tee shirt into a plastic or airtight container. I would store it in a cool location until it was delivered to the laboratory. Processing of item: 2. Handprint on car Collection of item: I would apply powder to the location of the handprint and then I would lift it with a broad piece of adhesive tape.…

    • 746 Words
    • 3 Pages
    Improved Essays
    Read More
  • Improved Essays

    Fingerprinting: The Dead Girls Of Hysteria Hall
    • 1079 Words
    • 5 Pages

    Fingerprinting: The Dead Girls Of Hysteria Hall

    D. Fingerprinting is an impression or mark made on the surface by a person's finger tip. It is used for identifying individuals from the unique pattern of whorals and lines. This fingerprinting is valuable to detectives case because the detective who has the mystery case, where a person was murdered would use fingerprinting. You would use fingerprinting to track down the murder and the bystander.…

    • 1079 Words
    • 5 Pages
    Improved Essays
    Read More
  • Improved Essays

    Grim Sleeper: A Forensic Analysis
    • 1040 Words
    • 5 Pages

    Grim Sleeper: A Forensic Analysis

    The advancement of forensic science has progressed immensely since its conception. The contributions it has made for the legal system are immense. One such example are its techniques used to extract DNA during forensic cases. DNA is considered to be one of the most well regarded and highly assessed sources of information (Gershaw et al., 2010). Another important development used during investigations is CODIS or the Combined DNA Index System.…

    • 1040 Words
    • 5 Pages
    Improved Essays
    Read More
  • Improved Essays

    Gel Electrophoresis Lab
    • 621 Words
    • 3 Pages

    Gel Electrophoresis Lab

    For example, gel electrophoresis is used to solve crimes where a DNA sample of the possible suspects involved are available. The sample can be used to match a suspect to a crime scene. Furthermore, the process lets researchers separate and purify DNA proteins so they can be studied in further detail. In this experiment, we will build a gel electrophoresis, and use it to examine how many components are in varying colors of food coloring dye. The dye that will be examined will represent DNA proteins.…

    • 621 Words
    • 3 Pages
    Improved Essays
    Read More
  • Improved Essays

    Science Long Gone Case Study
    • 954 Words
    • 4 Pages

    Science Long Gone Case Study

    1. Summarize key milestones involved in the past and present shaping and transitional dynamics behind changes in the present health care industry. Deoxyribonucleic acid also known as DNA is our genetic identification. Once thought by students, as a boring waste of time, has made leaps and bounds in the health care industry. DNA fingerprinting not only proves paternity, but it is also useful for crime scene investigations.…

    • 954 Words
    • 4 Pages
    Improved Essays
    Read More
  • Improved Essays

    The Pros And Cons Of The DNA Evidence
    • 1066 Words
    • 4 Pages

    The Pros And Cons Of The DNA Evidence

    In conducting the DNA test, the criminalist is able to extract a genetic “fingerprint,” which can then be compared with the genetic fingerprint of a DNA sample taken from the accused (Garland, 2011 p 135). Both DNA evidence and blood grouping evidence are forms of statistical proof –DNA evidence being the most useful in determining identity. For example, blood grouping evidence may narrow the range of other potential suspects with the same blood type to one out of two hundred, whereas DNA evidence may, as a practical matter, eliminate any possibility that there exists another potential perpetrator by identifying the specific genetic “makeup” of the accused (Garland, 2011 p…

    • 1066 Words
    • 4 Pages
    Improved Essays
    Read More
  • Improved Essays

    Dna Technology Research Paper
    • 488 Words
    • 2 Pages

    Dna Technology Research Paper

    DNA inputting introduces two important matters for judges. First the judge must decide if the DNA is admissible and then the jurors must understand the proper requirement for "weighing" evidence (National Research Council). There are two principles on which DNA is decided for as admissible or not. First the Frye test and the other is "helpfulness." The Frye test came about from the case Frye v. United States.…

    • 488 Words
    • 2 Pages
    Improved Essays
    Read More
  • Improved Essays

    Dna Testing Research Paper
    • 770 Words
    • 4 Pages

    Dna Testing Research Paper

    Updating capacity and infrastructure would help with DNA testing. The update of infrastructure, automating DNA analysis procedure, and improve storage of forensic evidence would help with DNA identification ("Advancing Justice" 3). According to the article, there are ways to help with DNA testing, but there is no action being taken to do so. Most crime labs do not have the capacity to analyze DNA in a timely fashion. Upgrading capacity would help ("Advancing Justice" 3).…

    • 770 Words
    • 4 Pages
    Improved Essays
    Read More
  • Great Essays

    Informative Essay: What Is Genetic Testing?
    • 1404 Words
    • 6 Pages

    Informative Essay: What Is Genetic Testing?

    If you had told someone in the early 1800’s that every cell in your body contains something called DNA made of molecules called nucleotides (DNA) that determine every characteristic about you, from your height and eye color to whether or not you’ll get a certain disease or condition, they would think you were crazy. Today, most students learn about DNA in middle school biology. DNA was first discovered by a German biochemist named Frederich Miescher in 1869, but its importance was not realized until 1953 (DNA). Genetic testing, “a type of medical test that identifies changes in chromosomes, genes, or proteins” (What is Genetic Testing?), however, was first done in the 1910s with ABO blood typing (O’Neil). Today, testing is used for determining paternity, determining a person’s chance of developing or passing on a genetic disorder, and to confirm or rule out a suspected genetic condition (What is genetic testing?).…

    • 1404 Words
    • 6 Pages
    Great Essays
    Read More
  • Improved Essays

    Microscopic Hair
    • 449 Words
    • 2 Pages

    Microscopic Hair

    Making it more suitable for genetic markers to be used when processing evidence in the lab. Fingerprint examiners must check the results numerous times to prevent false convictions and to maintain the integrity of the science (Bertino 140). Growth of DNA technology has impacted microscopic hair comparison (Dale and Becker 80). Hair is tested macroscopically, in which the length, colour and curliness are examined; and microscopically, in which the pattern of the medulla, pigmentation of cortex and even the type of scales on the cuticles are examined (Bertino 56). A comparison microscope allows for side-by-side comparison of samples.…

    • 449 Words
    • 2 Pages
    Improved Essays
    Read More
  • Great Essays

    DNA Sampling Controversy
    • 1456 Words
    • 6 Pages

    DNA Sampling Controversy

    Technology has made a major impact on the world we live in today keeping it both current and informative. It serves a great purpose providing various benefits. However, the use of technology can also raise some controversy. The advancement of DNA technology is imperative and it has significantly changed the legal system in the way cases are questioned, investigated and solved. On the other hand, DNA sampling raises issues involving privacy and the violation of human rights due to its personal, unique makeup and the fact that it is stored in a database.…

    • 1456 Words
    • 6 Pages
    Great Essays
    Read More
  • Superior Essays

    Essay On Dna Profiling
    • 1980 Words
    • 8 Pages

    Essay On Dna Profiling

    After his discovery, he said “My life changed on Monday morning at 9:05 am, September 10th, 1984. What emerged what the world’s first genetic fingerprint” (3). Since it was first discovered in 1985, DNA profiling has had a significant impact on society and has changed forensic science for the better by expanding the amount of information we are capable of obtaining, making it a turning point in history. Scientific Foundation of DNA Profiling…

    • 1980 Words
    • 8 Pages
    Superior Essays
    Read More
  • Improved Essays

    Gel Electrophoresis Essay
    • 288 Words
    • 2 Pages

    Gel Electrophoresis Essay

    DNA Gel Electrophoresis Electrophoresis is a process in which macromolecules are separated by utilizing their electrical charge and size. A technique for separating protein molecules of varying sizes in a mixture by moving them through a block of gel, as of agarose or polyacrylamide, by means of an electric field, with smaller molecules moving faster and therefore farther than larger ones. Gel electrophoresis of DNA is used in DNA profiling. When DNA is found at a crime scene, it's sent to a investigation lab.…

    • 288 Words
    • 2 Pages
    Improved Essays
    Read More
  • Improved Essays

    Advantages And Disadvantages Of Dna In Criminal Investigation
    • 1264 Words
    • 6 Pages

    Advantages And Disadvantages Of Dna In Criminal Investigation

    As DNA profiling technology advances, DNA evidence is being resubmitted from crimes that were committed years ago and a comprehensive test is conducted to find the potential suspect. Although identifying the perpetrator of the crime may be successful, DNA will have to be taken from other family members. This can cause private information about an individual’s ethnic background or parentage to be available to involved personnel’s which could lead to discrimination. Inaccurate results from DNA evidence has the possibility to put an innocent person to jail. Despite this, small samples are sent to multiple independent labs, which helps to reduce the possibility of an error which affect the final results.…

    • 1264 Words
    • 6 Pages
    Improved Essays
    Read More
  • Improved Essays

    Genetics Adaptation And Diversity
    • 872 Words
    • 4 Pages

    Genetics Adaptation And Diversity

    5. The isolated DNA can be used to diagnose genetic disorders, investigate forensic evidence…

    • 872 Words
    • 4 Pages
    Improved Essays
    Read More

Related Topics

Ready To Get Started?
Discover
Company
Follow
©2024 Cram.com
Privacy Policy |  About Ads |  Site Map |  Advertise  | 
  • Cookie Settings