Electrophoresis was then used to make analysis. During this lab activity, we were required to do human DNA fingerprinting which is a method used to identify individuals and to prove their relationships. Due to time and the length of this lab activity, the experiment was divided into part one: DNA Purification from Buccal Swabs, part two: PCR (polymerase chain reaction) amplification of the d1s80 VTNR locus from human genomic DNA and Part three: Analysis of PCR products using gel electrophoresis. We had a specific activity to carry out during each lab section.
PART ONE: DNA Purification from Buccal Swabs
The materials used for this lab section are listed below: