DNA sequencing (Sanger to NGS)
Introduction: The DNA sequencing methods have quickly evaluated for the forty last years and their finding was joined by breakthroughs in the field of Molecular biology (1). The first efficient method to sequencing DNA was found by F.Sanger in 1977 (2). But the huge desire to sequencing genomes required faster methods and many improvements were made. Fluorescence, capillary electrophoresis and microarrays led to a new way for sequencing genes : the Next Generation Sequencing (NGS). How do Sanger technique and NGS work ? What are their applications?
Sanger sequencing
The Sanger technique is based on the replication of DNA. The unknown sequence is inserted in a replication plasmid then it is cloned by E. coli (Escherichia
Introduction: The DNA sequencing methods have quickly evaluated for the forty last years and their finding was joined by breakthroughs in the field of Molecular biology (1). The first efficient method to sequencing DNA was found by F.Sanger in 1977 (2). But the huge desire to sequencing genomes required faster methods and many improvements were made. Fluorescence, capillary electrophoresis and microarrays led to a new way for sequencing genes : the Next Generation Sequencing (NGS). How do Sanger technique and NGS work ? What are their applications?
Sanger sequencing
The Sanger technique is based on the replication of DNA. The unknown sequence is inserted in a replication plasmid then it is cloned by E. coli (Escherichia