Molecular biology

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  • Polymerase Chain Reaction Lab Report

    Suppose we have a certain segment of a DNA molecule ,a gene for example that we want to amplify ,that is make many identical copies of that gene of interest, one way is to basically take that gene to integrate it into a bacterial plasmid to place that recombinant plasmid into a bacterial cell and to allow that bacterial cell to divide many times and eventually make many copies of that gene of interest. The problem with this particular method is that it is not only time consuming and not only is…

    Words: 1025 - Pages: 5
  • Genetically Modified Foods: Pros And Cons

    Genetically Modified Foods refers to plants which are produced by both animal and human consumption through the use of the modern molecular biology techniques. The plants have been changed in the laboratory so as to enhance the preferred features like better nutritional content or raise resistance to herbicides (Arvanitoyannis and Krystallis, 2005). The modification of the desired features has traditionally been carried out through breeding, but some of the breeding methods like conventional…

    Words: 831 - Pages: 4
  • Polymerase Chain Reaction (PCR)

    the PCR is to produce required amount of the target DNA region that is be analyzed. PCR amplified DNA is sent for sequencing and visualized by gel electrophoresis. It also can be cloned into plasmids. PCR is used in many fields of medicine, molecular biology research, medical diagnostics, and ecology. It is fast and inexpensive technique used to amplify DNA and RNA fragments by 107 times. The polymerase chain reaction was…

    Words: 916 - Pages: 4
  • Pglo Transformation Lab Report

    Genetic transformation is an important method, in molecular biology and genetic engineering, for transferring DNA amongst a variety of organisms. In Lab five, my lab partners and I used calcium chloride to make the bacterium cell walls more permeable and a heat shocking method to introduce the pGLO plasmid in the E.coli bacterium so that they may exhibit ampicillin resistance. The Goal of the experiment was to observe whether or not, given one of the four specific conditions, the pGLO plasmid…

    Words: 1027 - Pages: 5
  • Multiplex PCR

    4. Multiplex Polymerase Chain Reaction Multiplex PCR is molecular biology technique for amplification of multiple targets in a single PCR reaction. Indeed, multiplex PCR is a modification of conventional or realtime PCR in which two or more different PCR products are amplified within a single reaction (Henegariu, 1997). In a multiplexing assay, more than one target sequence can be amplified using multiple primer pairs in a reaction mixture. As an extension to practical use of PCR, this technique…

    Words: 1066 - Pages: 5
  • Nidulans

    Aspergillus nidulans: One of the lesser known pathogen of the aspergilli group, A. nidulans is a model filamentous fungus widely used for studying eukaryotic cell biology (Galagan et al., 2005). A. nidulans possesses a phospholipid-hydrolyzing novel cPLA2 protein, PlaA, which shows maximum similarity to mammalian-type cPLA2 proteins (α, β, γ) (Hong et al., 2005). Like the three isoforms of human cPLA2 proteins, A. nidulans PlaA also consists of two separate catalytic domainsA and B, and…

    Words: 1807 - Pages: 8
  • Unknown Plasmid Lab Report

    Identifying an Unknown Plasmid Through the Process of Gel Electrophoresis Introduction: Biotechnology requires certain techniques and methods that help identify plasmids, which can be used for forensics, DNA fingerprinting, etc. In this class, each lab focused on teaching the process of using the correct techniques used to identify a plasmid. Plasmids are pieces of DNA that are circular and relatively smaller than chromosomes. They aren’t important in the sense that they don’t carry out…

    Words: 1066 - Pages: 5
  • Gene Editing Ethics

    Recently, scientists in the UK have been given the green light to start research on editing the DNA of a human embryo. The ability to unzip defective genes and replace them with nondefective copies of genes has sparked a huge debate on the ethics of human gene editing. In this paper, I will briefly explore the procedure of gene modification using the editing tool CRISPIR/Cas 9, the exciting possibilities of successfully using this method, and debate several ethical concerns that have arisen due…

    Words: 1350 - Pages: 6
  • Essay On Agarose Gel Electrophoresis

    Background DNA molecules can be too big to analyze through electrophoresis, some they must be cut up into smaller pieces. In order to cleave dsDNA at the specific sites, making sure each fragment contain the desired sequence, restriction enzymes are used. Restriction enzymes can recognize a specific sequence of nucleotide and hydrolyze the bond of the DNA backbone. Hence, the fragments from the same DNA source will also be the same if cleave by the same restriction enzymes. Restriction enzymes…

    Words: 1222 - Pages: 5
  • Protein Analysis Lab Report Protein

    mobility and molecular weight. It can be seen more clearly that as the Rf value increased, the more the molecular weight decreased. 14.4 kD had the highest Rf of 0.926, compared to 94 kD with an Rf of 0.211. Figure 2: The semi-logarithmic graph above showed the relationship between relative mobility and molecular weight. Figure 3 showed the actual picture of the gel and its bands, noting the most important ones. Using Figure 2’s regression line, the most important bands’ molecular weight…

    Words: 863 - Pages: 4
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