Molecular biology

Ammonium sulfate precipitation, ion exchange chromatography, and SDS-Page were the three different techniques used in this experiment. The standard molecular weight of rubisco is 66 kDa and 14 kDa. rubisco was expected in P2 with nothing added. Also in P2 medium salt the evident of rubisco. T The standard coefficient of rubisco is between 14.1 ug/mL and 18.2 ug/mL (Hudson, 1994). The average of the two…

1) A. In 1947 Francis Crick began working with Max Perutz at the Cavendish Laboratory in Cambridge. He began working on a new sector of the Laboratory where X-ray crystallography was being used in order to study protein structure. Crick's thesis project at the university was on X-ray diffraction of proteins. Crick then met John Watson and together they solved the structure of DNA using available X-ray data at the time and model building. After discovering the double helix model, Crick and…

PCR is used to magnify the 16S rRNA gene and is used in molecular biology to make thousands of copies of the magnified DNA. There are three main stages that the PCR carries out, the first is denaturing when the double-stranded DNA is separated into two strands. Second, annealing which enables the DNA primers to attach…

A lot of people don’t even know what GMO’s are? Either they don’t care to know, or just too naive to figure out exactly what GMO’s are. I believe GMO’s are bad for humans, unless you’re sick which I’m not, I see GMO’s as a bad thing. GMO’s or “genetically modified organisms” these could range from animals to plants that have been genetically altered with DNA from plants, animals, bacteria, and viruses. The American Academy of Environmental Medicine (AAEM) advises medical physician’s to…

Plasmid Interference in Regulation of the Type III Secretion System in Pseudomonas aeruginosa Pseudomonas aeruginosa, an opportunistic pathogen, commonly colonizes patients with cystic fibrosis (1) and contributes to a sizeable proportion of antibiotic-resistant nosocomial infections (2). Acute P. aeruginosa infections are characterized by the expression of the type III secretion system (T3SS) comprised of a needle-like apparatus used to inject exoenzymes into neighboring cells (1, 3). These…

The first time I learned about structural biology was when I took Comprehensive Biochemistry I during my senior year at University of Maryland, Baltimore County. What was intriguing was how quickly I learned the concepts, and how easy it was for me to see conformational changes in three-dimensional space. I have always been a visual-oriented person, and until senior year of college I found that it was hard to find areas of research in biochemistry that utilize a person’s ability to analyze…

Gene Therapy: The concept of gene therapy emerged in the 1970s. The basic premise of gene therapy is using DNA enclosed in a vector as a therapeutic treatment for diseases. In the decades after it was conceptualized, many clinical trials of gene therapy were developed but stalled in phase I or phase II. During the 90s, only 1% of gene therapy trials made it to phase III and none of them went past phase III. The excitement for gene therapy was reduced when a participant of a clinical trial died…

Introduction and Social Relevance Our economy is ever-growing and evolving, this can be problematic for the agricultural crops that will have difficulty providing the world as the demand for food increases. Genetic modification is the use of biotechnology techniques to change the genes of an organism (AGRIC, 2015)
In Australia unless the Australian Gene Technology Regulator has concluded the Genetically Modified Organism, abbreviated as GMO is safe to human health as well as the environment it…

Think of the last time you purposely inflicted a bodily harm upon yourself, or a time when you put your own body at risk. Chances are you can’t think off a time when that happened, but what if that was false? Research shows that you may be harming your body by simply eating food. What are these foods you might ask? These harmful foods go by the name of Genetically Modified Organisms, or GMO’s. These relatively new organisms produced by mutating the natural genes are understudied and are in most…

The experiments covered in this report are based on the topic of DNA polymorphisms, these according to Houseman (1995) are differences in the DNA that occur between individuals. The polymorphisms under investigation here are classified as VNTR’s (variable number tandem repeats) and RFLP’s (restriction fragment length polymorphism). The first experiment is concerned with DNA polymorphisms that are known as VNTR’s, sections of DNA which have repeated nucleotide base sections in which the repeats…