Molecular biology

1.2 Cellular DNA Damage responses, genomic instability. DNA is the blue print of life and all the information of life processes such as growth, metabolism, reproduction etc are encoded in the sequence of it. Therefore its very important to maintain the genomic integrity of this genetic material, not only to keep away defects in life processes but to pass a faithful information to the next progeny. Integrity of the DNA is usually challenged by both endogenous and exogenous agents who are capable…

Medicinal plant is an organism of the kingdom Plantae; now specifically, a living organism of the embryophyta (land plants) or of the chlorophyta (green algae), a eukaryote that includes double-membrane bounded chloroplasts in its cells containing chlorophyll a and b. Medicinal plants contain a large variety of chemical substances that possesses important therapeutic properties used in the treatment of many diseases. Increasingly industrialized societies are developing drugs and…

DNA organization within cells is a complex and sophisticated process. This high level of complexity is due to the hundreds of thousands of interactions that can be enabled through organization. It is a known that promoter elements and enhancer elements work in unison to regulate gene expression. Often times enhancers are found hundreds of kilo-bases away from their interacting promoter elements. These enhancers initiate promoter activation via interactions amongst transcription factor binding…

Plants reproduce by asexual reproduction or pollination in most cases, they do this in order to make seeds. Seeds are the embryonic, or unborn, state of a plant. These seeds are made to carry the genetic material and make new plants. In most cases a plant wants its seeds to be spread of moved to a new place not close to the parent plant, this is done through seed dispersal. There are many types of seed dispersal for example wind, water, animals, and other biotic and abiotic factors. One of the…

3.0 MATERIALS AND METHODS 3.1 Materials The main material that used was sodium dodecyl sulphate and Zebrafish embryo. SDS was purchased from Sigma Aldrich with concentration of 99.95%. The embryo of the zebrafish larva was obtained by breeding the adult zebrafish in the laboratory. Furthermore, the other basic materials that was used including aquarium tanks, breeding tanks, water filter, fish food pellet and Instant Ocean Salt. While for the measurement tools that used were pH meter, cylinder,…

6F4Z1003- Genetics, Adaptation and Diversity Practical Report Section 1- Calculations and Pedigrees 1. In DNA extraction the proteins absorb light at 260nm and 280nm, especially the aromatic acids. 2. 250µg to nm, 250 x 1000 = 250,000ng. 3. 200ng/ml = 0.2µg/ml. 150µg/ml = 150,000µg/ml 250,000pg/µl = 250µg/ml 1mg/ml = 1000µg/ml ● 150,00µg/ml - HIGHEST CONCENTRATION OF DNA ● 1000µg/ml ● 250µg/ml ● 0.2µg/ml - LOWEST CONCENTRATION OF DNA 4. Sodium dodecyl sulphate is used as a…

Today, I concluded the experiment of last meal mix up. Through multiple test, I was able to decipher which stomach content belongs to what person. The first test was the brown paper bag test, which allows me to see if the stomach content contains lipids or not.. This involved taking a piece of bag paper and using a pencil to divide it into five sections. Next, I labeled each section with the number of the stomach contents. Then, I placed two drops of each sample in the appropriate section. After…

DNA analysis, also known as DNA profiling, testing, typing, is a process that takes genetic material and evaluates it so that it can identify individuals in a criminal investigation or in use of a forensic application. The beginning step of the performance of DNA analysis on a reference sample or person is the collection of DNA from cells. These cells can come from a blood sample or even swabbing the inside of an individual’s cheek. After it is collected, the samples are then sent to a lab for…

Many endosymbiotic theories have figured in opinion about the differences between prokaryotic and eukaryotic cells. More than 20 different versions of Endosymbiotic theories have been presented in literature to explain the origin of eukaryotes and their mitochondria (Martin, Garg and Zimorski, 2015, p.1678). A Prokaryotic cell contains structural components such as: Cell wall is made of peptidoglycan. Gram-positive bacteria contain a thick cell wall with many layers of peptidoglycan…

a very interesting read that introduced new ideas to me. Many of the ideas in the book applied directly to our Biology class. Some topics that were covered in lecture can be directly applied to the book. Matt Ridley talks about many important things in his novel; however, I feel as if the chapters of life, species, and environment are the most relatable to lecture as well as our biology class as a whole. First and foremost, Ridley addresses life in his first chapter. This chapter consisted of…