Green fluorescent protein

Green Fluorescent protein and Green Fluorescent protein are suggested to be involved in bacterial transformation tool, which helps the scientists visualize the normal protein in cell. To test the hypothesis, the experiment had been collected the around 15 colonies from E.coli source plates. Those amounts of E.coli, then, were used to test under transformation of E.coli with Green and Blue Fluorescent Proteins procedure. As the result, the E.coli that had been injected pFluoroGreen and pFluoroBlue will be survived in the Ampicillin environment, and they can express the fluorescent characteristic supporting by the IPTG, which helps to release the RNA polymerase (T7). The results also shows that the transformation efficiency rate of the class…

Literature Review: Green Fluorescent Protein and Histidine Tagged Proteins Histidine and green fluorescent protein (GFP) tagging are important in molecular biology because they allow for purification, tracking, and quantification of target proteins (Ferrari et al., 2004; Cho et al., 2011; Deponte, 2012). Histidine tagging provides a method for isolating and increasing the amount of target protein recovered from a biological organism or mixed sample of proteins (Masek et al., 2011; Singh and…

The study of the brain began by mapping structures such as lobes, cortexes, and regions. As modern medicine continues to make advancements so does the understanding of complex processes which take place inside of the human body such as the firing of action potentials in neuronal cells in the brain. The research presented in the paper “High-Fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor” shows just how far brain mapping has come and how far…

Many neurological and psychiatric disorders are associated with or directly caused by deficits in synaptic protein availability, morphology, or physiology. Our technology will enable system-level understanding of biomolecules within or around a synapse, providing critical information that will advance the diagnosis of brain diseases and support development of new brain therapeutics. References 1 Nakai J, Ohkura M, Imoto K. A high signal-to-noise Ca(2+) probe composed of a single green…

an important role in experimental studies. His tagging is widely used for purifying and detecting large amounts of targeted proteins (Kaplow et al., 2009). GFP tagging can aid in the analysis of protein geography, movement and chemistry in a living cell (Lippincott-Schwartz and Patterson, 2003). A His-tag is a sequence of approximately six histidine residues that is usually attached to either the N- or C- terminus of the protein being targeted (Loughran and Walls, 2010). The purification process…

cells. When these vectors transfer the targeted cells, the genes are expressed, resulting in the production of therapeutic proteins and thus treating protein deficiency associated with the disease. However, both the vectors and the method of delivery draw great safety concerns. First, viral vectors can be toxic and yield unpredictable side effects. When delivered into the blood stream, these side effects become irreversible and permanent. As a result, despite the amount of clinical trials…

Purpose: The overall goal of this lab was to perform a procedure on E. Coli which involved transferring genes that encoded for the green fluorescent protein into E. Coli to see if the transferred genes would make a difference on the growth and whether or not the bacteria would glow under UV light. Hypothesis: If the bacteria with the pGLO plasmid was grown on a plate containing LB and ampicillin then the bacteria will grow but not glow under UV light. If the bacteria with the pGLO plasmid was…

Imagine having glowing parts at night in your body. Having the ability to glow in the dark would be fun. There are many things in the world that glow in the dark. For example, glow sticks, lightning, fireworks, mushrooms, bacteria, algae and many more. The Aequorea Victoria Jellyfish is a jelly that glows in the dark. I am more than interested to learn how luminescence works and its processes. The Aequorea Victoria Jellyfish is a jellyfish found in the Puget Sound, Washington State. The…

An operon is a cluster of genes in a bacteria that are clustered together and have related functions. Genes in an operon are coexpressed, which means that they are turned on or off under a single promoter. The genes of the arabinose operon are Ara A, Ara B, and Ara D (or the B. A. D. genes) which are all digestive enzymes that allow bacteria to break down arabinose. When Ara A, Ara B, and Ara D cluster together they form the arabinose operon. The gene that replaces the arabinose operon is the…

DNA analysis by By Condy Kan Introduction Osamu Shimomura and his colleagues successfully discovered and isolated green fluorescent protein (GFP) from the jellyfish Aequorea victoria; GFP consists of a tripeptide, which is Ser-65, Tyr-66, and Gly-67, that forms fluorescence chromophore (1). Fluorescence chromophore, is accountable for its green fluorescence under UV light, helps to identify and determine the cells if the Enhanced Green Fluorescent Protein (EGFP) gene is expressed or not (2). In…