Purpose: The overall goal of this lab was to perform a procedure on E. Coli which involved transferring genes that encoded for the green fluorescent protein into E. Coli to see if the transferred genes would make a difference on the growth and whether or not the bacteria would glow under UV light.
Hypothesis: If the bacteria with the pGLO plasmid was grown on a plate containing LB and ampicillin then the bacteria will grow but not glow under UV light. If the bacteria with the pGLO plasmid was grown on a plate containing LB, ampicillin, and arabinose then it will be able to grow and glow under UV light. If the bacteria without pGLO plasmid was grown on a plate containing LB and ampicillin then it will not be able to grow or glow under UV light. …show more content…
The hypothesis about the +pGLO plate containing ampicillin and LB was correct because the bacteria did end up growing and not glowing under UV light. It ended up growing because the LB that was in the plate with it helped the bacteria grow and reproduce. The bacteria became resistant to the ampicillin because the DNA plasmid contained beta lactamase which made the cell resistant to ampicillin. It did not end up glowing under UV light because the gene for the green fluorescent protein was repressed by the araC gene found on the DNA plasmid. The hypothesis about the +pGLO plate containing LB, ampicillin, and arabinose was correct because the bacteria did end up growing and glowing under UV light. The bacteria grew because there was a source of food present to help the bacteria grow and reproduce and there was beta lactamase in the DNA plasmid which allowed the cell to become resistant to ampicillin. It ended up glowing after the procedure because the arabinose allowed the green fluorescent protein in the DNA plasmid to be expressed. The araC gene that repressed the green fluorescent protein became repressed by arabinose. The hypothesis about the -pGLO plate containing LB and ampicillin was correct because the bacteria did not grow or glow after the procedure. It did not grow because there was ampicillin present on the plate, which interfered with the cell’s ability to synthesize the cell’s walls and made cell growth impossible. Although there was food present for the bacteria, the ampicillin contradicted with growth and reproduction. The fact that there was no DNA plasmid had a role in the bacteria not glowing. Since there was no plasmid on the plate, the gene for green fluorescent protein was not even present, so there was no way for the bacteria to glow. The hypothesis about the -pGLO plate was correct because the bacteria ended up growing but not glowing under UV light. The bacteria had such a prominent growth rate
Hypothesis: If the bacteria with the pGLO plasmid was grown on a plate containing LB and ampicillin then the bacteria will grow but not glow under UV light. If the bacteria with the pGLO plasmid was grown on a plate containing LB, ampicillin, and arabinose then it will be able to grow and glow under UV light. If the bacteria without pGLO plasmid was grown on a plate containing LB and ampicillin then it will not be able to grow or glow under UV light. …show more content…
The hypothesis about the +pGLO plate containing ampicillin and LB was correct because the bacteria did end up growing and not glowing under UV light. It ended up growing because the LB that was in the plate with it helped the bacteria grow and reproduce. The bacteria became resistant to the ampicillin because the DNA plasmid contained beta lactamase which made the cell resistant to ampicillin. It did not end up glowing under UV light because the gene for the green fluorescent protein was repressed by the araC gene found on the DNA plasmid. The hypothesis about the +pGLO plate containing LB, ampicillin, and arabinose was correct because the bacteria did end up growing and glowing under UV light. The bacteria grew because there was a source of food present to help the bacteria grow and reproduce and there was beta lactamase in the DNA plasmid which allowed the cell to become resistant to ampicillin. It ended up glowing after the procedure because the arabinose allowed the green fluorescent protein in the DNA plasmid to be expressed. The araC gene that repressed the green fluorescent protein became repressed by arabinose. The hypothesis about the -pGLO plate containing LB and ampicillin was correct because the bacteria did not grow or glow after the procedure. It did not grow because there was ampicillin present on the plate, which interfered with the cell’s ability to synthesize the cell’s walls and made cell growth impossible. Although there was food present for the bacteria, the ampicillin contradicted with growth and reproduction. The fact that there was no DNA plasmid had a role in the bacteria not glowing. Since there was no plasmid on the plate, the gene for green fluorescent protein was not even present, so there was no way for the bacteria to glow. The hypothesis about the -pGLO plate was correct because the bacteria ended up growing but not glowing under UV light. The bacteria had such a prominent growth rate