Green fluorescent protein

transformation process into Escherichia coli (E. coli). pGLO carries several genes such as β-lactamase, green fluorescent protein, origin of replication, and transcriptional regulator gene that affects transformation. For instance, the β-lactamase gene (bla) gives the transformed bacteria resistance to ampicillin. The green fluorescent protein gene (gfp) will provide the transformed bacteria with a green glow. The origin of…

to induce the competence of the bacterium. Successful transformation results were expressed by phenotypic characteristics such as growth in the medium and glowing from exposure of UV light from the pGLO plasmid genes (β-lactamase gene and green fluorescent protein gene). Introduction Genetic transformation…

ingredients. One was labeled “+DNA,” the other with “-DNA.” They were both filled with different bacteria. More specifically, the “+DNA” tube had pGFP in it and included a plasmid. Included on that plasmid were two genes: gfp (gene for green fluorescent protein) and an ampicillin resistant gene. The gfp gene makes cells glow and the resistant gene fights against ampicillin, which kills cells. This plasmid DNA was added to half of the cells before both tubes went through a competency process.…

The main objective of the experiment is to study and localize the fluorescent tags on specific chimeric proteins in a particular cell. The localization of these tags will generate a detailed understanding of the cytoskeleton in the cell, and the main function of using the Enhanced Yellow Fluorescent Protein. With the help of EYFP, the location of the proteins, and the molecular masses of the chimeric proteins should be really easy (Cannons, A. 2006). Consequently to better understand this…

adaptation and survival, but it is only successful if all substances needed are in the mixture as well (Masterman 2010). My hypothesis is that using the heat shock method described above, the plasmid pGLO will be used as a vector to transfer green fluorescent protein, arabinose C, and ampicillin resistance from the pGLO to the E. coli bacteria and will present itself by glowing in the presence of arabinose and surviving on media that contains ampicillin. The significance of this experiment is to…

Bacterial Transformation Ian Nall BIO 181 | Section #73116 | Wednesday 1:30-4:15pm Abstract This experiment was designed to test the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed in either…

Engineers are always applying their knowledge of Mathematics and Science to invent efficient solutions to technical problems. During this process, some engineers decide to file a patent application. A patent is a legal document issued by the federal government that provides protection for an idea. The patent allows engineers to prevent others from making, using, or selling their patented invention. Patent can tell the story of the invention in a way that someone could make the invention using…

Question 7.2 Cite some experimental evidence that indicates that membrane proteins drift. A good form of experimental evidence is Fluorescence Recovery After Photobleaching, aka FRAP. In the FRAP process, membrane proteins are labelled with a green fluorescent protein, and part of the plasma membrane is “bleached” with a laser, causing them to lose their fluorescence. The part that was bleached will eventually become fluorescent again, as molecules drift in by diffusion. How might the membrane…

DNA known as protospacers indicative of particular bacteriophages encountered by bacteria. These sequences are then used as a template for RNAs capable of recognizing viral DNA within a cell and as a means to bind the endonuclease CRISPR associated protein 9 (Cas9). These complexes allow the enzyme to be shuttled to the target DNA to perform a cut on one or both strands of the foreign DNA in either of the enzyme’s two active sites. Recently, scientists have repurposed this bacterial defense…

Caenorhabditis elegans C. elegans is a nematode worm that considers as one of the simplest multicellular model organism. It is a free-living soil animal that feeds on microbes in decaying plant environments while in vitro, it mainly feeds on bacterial lawn (40). Sydney Brenner has proposed C. elegans as an experimental model organism and it has been used in biological fields, it played a huge role in the understanding and development in the knowledge about multicellular organisms since 1960s (8)…