Green fluorescent protein

and consecutively 50 ng of cDNA was used for quantitative real time PCR assay. cDNA was amplified in a thermal cycle (CFX96 PCR system, BioRadLaboretories) in 20 µl reaction mixture containing 0.5 µl cDNA template (50 ng), 12.5 µl Maxima SYBR green/fluorescent qPCR master mix (2X, Thermo Scientific), 0.3 µl forward and reverse primer (100 pmol), 6.4 µl nuclease free water. The details of primer sequences, sizes of PCR products, and the annealing temperature are listed in Table-1. PCR…

negotiation power remains low. We also started to enter the Modern Trade, which till now was not explored by the company and started with an exclusive distributor for smaller modern trade stores. This distributor later also helped in selling of Compact Fluorescent Lamps in huge volumes. These efforts also attracted 5 Havells Galaxies in the region, all products exclusive Havells…

In 1937, the Spanish government commissioned Pablo Picasso to paint an oil on canvas mural for the 1937 World Fair in Paris, France. Guernica is a large piece, sitting at twenty-six feet wide and eleven feet tall, and was placed at the entrance of their pavilion. Picasso found inspiration from reading about the bombing of Guernica, Spain by the German air force, and although not a complete portrayal of the event, Picasso conveys the suffering and agony of the people and the tragedy of the war.…

spectrally tunable and “color changing”, full-color tunable products often consist of 3 or more LED sets. The individual LEDs can be narrow-band LEDs (producing a “pure” color, usually RGB), or coated LEDs. The monochromatic LEDs are usually red, green and blue (the primary colors of light, RGB), and can be augmented with amber (A) and white (W). The King Blade is a typical RGBW full-color tuning product. Full-color tuning is being used to create theatrical effects in live concerts, parties,…

type of fire lamp. In 1841 Arc-lighting, 1854 incandescent lamp by passing an electric current through a carbonized bamboo filament, 1867 coated electric discharge tubes with luminescent materials, a process that was further developed in later fluorescent lamps. Now keep in mind this technology is adapting, and modifying as each person; each civilization modifies this…

The discovery of electricity and the invention of the lightbulb in the mid-19th century revolutionized the American society, politics, and culture. During the mid 19th century life was different: candles were the only source of light, transport was steam-powered, food was stored in ice boxes. However, within the next fifty years, electricity would change that. In 1878, Thomas Edison invented the incandescent lightbulb; in 1888, Frank Sprague’s electric trolley system became the first…

Honor is to regard with great or respect or to have high esteem for someone or something. My personal definition of honor is to be a productive member in society and to help out others around you. Thomas Edison In 1877 Thomas Edison invented the first phonograph. A phonograph is a sound reproducing that uses cylinders to record sound as well as reproduce sound. In 1879 Thomas Edison built the first commercially practical incandescent light bulb. Edison also introduced the world's first viable…

Infrared, fluorescent, and LED lights, and they all can do different things. Ultraviolet light can be used as a way to light up certain types of clothing, or it can be used to sterilize food. Infrared light is mainly used as a way to heat up food, or as a shorter wavelength to control household items, such as televisions and DVD players. Fluorescent light is created by an electric current running through types of stable gas, which in turn produces short wave ultraviolet light, or fluorescent…

consist of thiol head group and terminal mannose, respectively. The SAMs of mannose prepared by this dual click strategy was found to be very effective in reducing the nonspecific interactions while specifically and selectively capturing green fluorescent protein (GFP)-tagged E. coli strain (pPKL1162) evident by epifluorescence micrographs with and without terminal mannose, Figure XYZ. The same strand of E. coli was also successfully detected on self-assembled dendritic monolayer (SADM) having…

There are many people that are dealing with depression and here are some ways to help combat depression without medication. Whether you take medication for depression already or not, there are things you can do to help you deal with it. If you try some of these ideas to fight depression you'll notice a difference and an improvement. What have you got to lose but those blues? 1. Listen to upbeat positive fast types of music. Avoid slow love songs or blues or jazz. The type of music you listen to…