Analytical phonics

The film, “Jaws”, was released in 1976, by Stephen Spielberg and is widely considered the first Hollywood Blockbuster. “Jaws” is one of my if not my favorite movie, I have seen this film, as many as a half dozen times and each time I view it, I find that I enjoy it more and more. “Jaws” has a unique way of captivating any audience who views the film, its director Stephen Spielberg, is a master at grabbing the audience’s attention by making us feel as if we are the ones in the water at the Amity…

Introduction: LC-MS is used for the bioanalysis of small molecules drugs, which has molecular mass of less than 800 Da. Now a days, it is also frequently used for the bioanalytical determination of biopharmaceuticals.1 In pharmaceutical R & D, it takes a year to develop a new marketing drug identified from thousands of new compounds. The drug discovery is divided into early and late phase, during which pharmacokinetics study or bioanalysis of a drug is done. Once, the bioanalysis of a drug is…

ABSTRACT Flakiness & Elongation index is one of the most prominent criteria that govern behavior and performance of aggregate in the bituminous mixes. The strength and serviceability requirements of bituminous mixture such as Stability, Flow, Voids in Total mix (VTM), Voids Filled with Bitumen and (VFB) highly depend on the physical properties of aggregates. This study conducted by observing the effect of flakiness & elongated index by adding different percentages from 0% to 50% of flaky &…

Introduction Thin-layer chromatography, otherwise referred to as TLC, is a fast and inexpensive way to determine the amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distances when eluted with the proper solvent. According to ChemistryGuide, this is because the more polar compound has a stronger interaction with the silica gel due to its…

The human body produces various essential proteins that provide functionality in the biochemistry of our system. How individual molecules are separated or a mixture of molecules are separated can be dependant of their size. Size exclusion chromatography (SEC), is the controlled separation technique that is based on the size of the particles in relation to the pore size (Barth and Saunders 2012). The separation process veers to be quick for large molecules and slower for smaller molecules. The…

Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged…

1.0 REFERENCES 1.1 Sample Preparation and Calculations for Dissolved Gas Analysis in Water Samples Using a GC Headspace Equilibration Technique, EPA National Risk Management Research Laboratory, RSKSOP-175, Rev.2, May 2004. 1.2 Light Hydrocarbons in Aqueous Samples via Headspace and Gas Chromatography with Flame Ionization Detection (GC/FID), Pennsylvania Department of Environmental Protection Bureau of Laboratories, PA-DEP 3686, Rev.0, April 2012. 1.3 Lange’s Handbook of Chemistry, 14th edition…

Carl Jung was a Swiss psychiatrist who established the body of analytical psychology. He stated that every person has several personalities within oneself. Basically, we can look at one’s personality from four different categories, persona, shadow, anima/animus and archetypes. The easiest to recognize is persona. Persona is a social mask that we all show to the public. We change the roles in different situations in society. For instance, a student acts as an attentive respectful person who…

air-dried for 2 days at room temperature. The collected leaves (Fig. 1) were freeze-dried immediately after sampling (harvest dates:30th March, 10th April, 28th April, and 12th May, 2013) and stored at –40 ºC prior to analysis. [Insert Figure 1] Analytical grade water, acetonitrile, and TFA were purchased for UPLC analysis from J.T. Baker (Phillipsburg, NJ, USA). For quantitative analysis, phenolic compounds used for the standards were isolated from barley leaves, as reported in earlier…

Introduction Chromatographic process is a separation technique which has to do with the mass transfer of test sample between a stationary and mobile phase. HPLC (High Performace Liqud Chromatography) is a vast system which involves the use of a mobile and stationary phase to separate materials. The stationary phase is usually a column packed with solids (usually silica gel) while the mobile phase is usually a solvent or a mixture of various solvents. The mobile phase usually, is the carrier of…