Thin layer chromatography

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  • Thin Layer Chromatography Essay

    Identification of Analgesic Drugs by Thin-Layer Chromatography 1 (a) In thin-layer chromatography alumina or silica is used as the adsorbent material. (b) The filter paper is placed inside the beaker during separation to act as an adsorbent medium. (c) The ultraviolet lamp is used after TLC separation to illuminate the TLC sheet. (d) Aspirin, acetaminophen, and caffeine are components of analgesic drugs examined in this experiment. 2 A 6cm by 10cm pre-coated TLC sheet will be obtained. On the powdery surface of the TLC sheet, a light pencil line 1cm from the TLC sheet will be drawn. On this light pencil mark, five small dots will be drawn, 1cm between the dots and 1cm from the edges of TLC sheet. On the top of each drawn dot, will be marked as A (aspirin), Ac (acetaminophen), C (caffeine), M (mixtures of the three analgesic drug components) and U (unknown analgesic drug). The fine capillary tube will be used to apply analgesic drug components on the dots. For instance, the fine capillary tube will be dipped in a stock aspirin solution, followed by touching the tip of the capillary tube and quickly transferring the solution to the dot marked A. The solvent at dot marked A will be allowed to evaporate. After evaporation of the solvent, another drop will be added to the same spot. This treatment will be conducted to other dots, but using separate fine capillary tubes and different stock solutions of analgesic drug components. 9cm filter paper will be placed against the wall…

    Words: 483 - Pages: 2
  • Thin Layer Chromatography

    liquid/liquid extraction. Experimental: Similar to the “Thin Layer Chromatography” experiment, a TLC plate was ran with 2 ml of EtOAc and 8 mL of hexane in the jar. The Rf value was calculated using the formula located in the data for all the TLC plate. The mixed compound that was dotted on the TLC plate had 0.300 g of the impure benzoic acid and 10 mL of dichloromethane. A 50 mL flask was weighed and recorded. A separation funnel was then filled with 10mL of 3M NaOH and 10 mL of the…

    Words: 1134 - Pages: 5
  • Thin Layer Chromatography Analysis

    through thin-layer chromatography analysis. By comparing the Rf values obtained from each TLC plate, it was deduced which components were present in the unknown drug. Through comparison of Table 1 and Table 2, Unknown A was identified as B.C. Tablets. Salicylamide was evident in the unknown substance due to the distinct blue color that was present in both the unknown spot and the salicylamide spot; the similar Rf values provided further evidence for the presence of salicylamide. Given that B.C.…

    Words: 865 - Pages: 4
  • Essay On Thin Layer Chromatography

    According to Chemguide, Chromatography is a physical method that is used to separate a mixture of chemical substances into its components, which then can be closely analyzed. All forms of chromatography share this same principle. Here, a mobile phase travels through a stationary phase, carrying the components of the mixture with it. It is possible that the mobile phase and the stationary phase have similarities or differences that affect the overall interaction between each other and the…

    Words: 1645 - Pages: 7
  • Thin Layer Chromatography Lab Report

    The purpose of this experiment was to determine the presence of the analgesic compounds acetaminophen, aspirin, caffeine, and salicylamide in the over-the-counter medications Anacin, Bufferin, Excedrin, and Tylenol. Thin-layer chromatography (TLC) was used to analyze solutions of the medications; the TLC plates were visualized using ultraviolet light and iodine staining.The ten micropipets used to spot the TLC plates were made from glass capillary tubes by heating the middle of each tube in a…

    Words: 1415 - Pages: 6
  • Difference Between Skittles And Chromatography

    Methods We began this experiment by laying out the needed materials to find the chromatography of Skittles Sweets and Sours and Crayola markers. Using a ruler and a pencil, a line was drawn on all thirty chromatography strips 1.5 centimeters from the bottom of the paper. This was to ensure that the starting point for each chromatography process was consistent. The chromatography of the skittles was found first. We began by pouring water into a cup. A pipette was used to transfer fifteen…

    Words: 2116 - Pages: 9
  • Trichophyton Rubrum Case Study

    (2015), Trichophyton rubrum (T. rubrum) is a dermatophyte responsible for causing the majority of superficial fungal infections worldwide. Dermatophytes are group of fungi that have the ability to invade keratinized tissues, such as hair, skin, and nails. This group of fungi can cause infection anywhere on the skin, however, they most commonly affect the inguinal region, feet, nails, scalp, and axillae. The infection results in mild to moderate dermatological symptoms, with a rang e of severity…

    Words: 1146 - Pages: 5
  • Synthesis Of 4-Nitrobromobenzene Lab Report

    products were formed: 4-nitrobromobenzene (4-NBB) and 2-nitrobromobenzene (2-NBB). Utilizing column chromatography and recrystallization, separation and purification of the two products was obtained. The mass of 4-NBB, the para product, was 1.57 grams, while the mass of 2-NBB, the ortho product, was 0.80 grams. The para product was expected to have the higher yield, since it is more stable and consequently harder to break down throughout the experiment. Keywords Nitration, Bromobenzene,…

    Words: 1570 - Pages: 7
  • Tlc Lab Report

    TLC and HPTLC Introductions Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent…

    Words: 728 - Pages: 3
  • Tlc And Column Chromatography

    Introduction Separation, a process by which the distinguishing between substances through physical or chemical means in a mixture may be carried out. A widely employed technique for this purpose is called chromatography. 1 There are many different types of chromatography, including gas-liquid, paper, column and so on. 1 Chromatography differentiates by allowing the differences in polarity, vapor pressure, solubility and so on of different substances to be observed. 1 In our endeavor, we will be…

    Words: 1030 - Pages: 4
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