Identification of Analgesic Drugs by Thin-Layer Chromatography
In thin-layer chromatography alumina or silica is used as the adsorbent material.
The filter paper is placed inside the beaker during separation to act as an adsorbent medium.
The ultraviolet lamp is used after TLC separation to illuminate the TLC sheet.
Aspirin, acetaminophen, and caffeine are components of analgesic drugs examined in this experiment.
A 6cm by 10cm pre-coated TLC sheet will be obtained. On the powdery surface of the TLC sheet, a light pencil line 1cm from the TLC sheet will be drawn. On this light pencil mark, five small dots will be drawn, 1cm between the dots and 1cm from the edges of TLC sheet. On the top of each drawn dot, will be marked as A (aspirin), Ac (acetaminophen), C (caffeine), M (mixtures of the three analgesic drug components) and U (unknown analgesic drug). The fine capillary tube will be used to apply analgesic drug components on the dots. For instance, the fine capillary tube will be dipped in a stock aspirin solution, followed by touching the tip of the capillary tube and quickly transferring the solution to the dot marked A. The solvent at dot marked A will be allowed to evaporate. After evaporation of the solvent, another drop will be added to the same spot. This treatment will be conducted to other dots, but using separate fine capillary tubes and different stock solutions of analgesic drug components. 9cm filter paper will be placed against the wall…
The purpose of experiment was to identify an unknown analgesic drug using thin-layer chromatography (TLC). Using the thin-layer chromatography to determine the composition of the Acetaminophen, Aspirin, Caffeine and Salicylamide, then compared with unknown analgesics.
Acetaminophen Aspirin Caffeine Salicylamide
C8H9NO C9H8O4 C8H10N C7H7NO
Figure 1. The Chemical structure of Acetaminophen, Aspirin, Caffeine…
Similar to the “Thin Layer Chromatography” experiment, a TLC plate was ran with 2 ml of EtOAc and 8 mL of hexane in the jar. The Rf value was calculated using the formula located in the data for all the TLC plate. The mixed compound that was dotted on the TLC plate had 0.300 g of the impure benzoic acid and 10 mL of dichloromethane. A 50 mL flask was weighed and recorded. A separation funnel was then filled with 10mL of 3M NaOH and 10 mL of the…
Thin-layer chromatography, otherwise referred to as TLC, is a fast and inexpensive way to determine the amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distances when eluted with the proper solvent. According to ChemistryGuide, this is because the more polar compound has a stronger interaction with the silica gel due to its…
through thin-layer chromatography analysis. By comparing the Rf values obtained from each TLC plate, it was deduced which components were present in the unknown drug. Through comparison of Table 1 and Table 2, Unknown A was identified as B.C. Tablets. Salicylamide was evident in the unknown substance due to the distinct blue color that was present in both the unknown spot and the salicylamide spot; the similar Rf values provided further evidence for the presence of salicylamide. Given that B.C.…
Thin Layer chromatography was also use to separate the mixture of fluorene and fluorenone. Thin layer chromatography is a useful separation method when the components of a mixture are in question. Analyzing what the mixture consist of can be efficiently determine by TLC by calculating Rf and comparing these values. For the separation and isolation of a mixtures components, this method is extensive and not as efficient at column chromatography. This conclusion is represented in the data gathered.…
According to Chemguide, Chromatography is a physical method that is used to separate a mixture of chemical substances into its components, which then can be closely analyzed. All forms of chromatography share this same principle. Here, a mobile phase travels through a stationary phase, carrying the components of the mixture with it. It is possible that the mobile phase and the stationary phase have similarities or differences that affect the overall interaction between each other and the…
Thin-Layer Chromatography Lab 11/12/14
To what extent does the polarity of a substance affect the distance it travels during TLC using water as the solvent?
The substances used during the TLC process could possibly be dangerous, so it would be best to avoid getting them on the hands. If one were to accidentally spill the water used in the process onto the floor, a slipping hazard could occur.
Solvent Front (cm)
The goal of this experiment was to use the technique of thin layer chromatography (TLC) to identify the active analgesic ingredient of Advil and monitor the transesterification of methyl salicylate from acetylsalicylic acid. The synthesized methyl salicylate was then purified using acid-base chemistry and analyzed using H NMR spectroscopy.
Identification of a proper mobile phase to separate common analgesics used in over-the-counter pain relievers was essential in being able to determine the…
In lab, a sample of crude caffeine was purified using the technique of sublimation. Thin layer chromatography (TLC) was used to verify the caffeine's chemical structure and purity. Sublimation played a huge role in the purification of the crude caffeine. The process of sublimation is to take a chemical and change it from a solid phase directly into a vapor. Another reason as to why sublimation was used is because the caffeine can be separated from its contaminant due to the fact that it does…