Thin layer chromatography

Purpose The purpose of the experiment Thin Layer Chromatography was to analyze compounds, Aspirin, a mixture of Tylenol with ethanol, acetaminophen, a mixture of Excedrin with ethanol, Aspirin, and acetaminophen, Anthracene, Benzyl, and Triphenylmethanol by separating them with the help of silica plates. Procedure Three silica plates were taken and on the plastic side, they were all marked 1 cm above its end using a pencil. With the help of capillary tube, the first and the second plates were…

The purpose of this experiment was to determine the presence of the analgesic compounds acetaminophen, aspirin, caffeine, and salicylamide in the over-the-counter medications Anacin, Bufferin, Excedrin, and Tylenol. Thin-layer chromatography (TLC) was used to analyze solutions of the medications; the TLC plates were visualized using ultraviolet light and iodine staining.The ten micropipets used to spot the TLC plates were made from glass capillary tubes by heating the middle of each tube in a…

experiment was to synthesize, purify, and identify p-nitroacetanilide using acetanilide as the starting material. To test for the purity and identification of our desired product, we gathered data from: Thin Layer Chromatography (TLC), Melting Point (MP) range, Infrared (IR) spectrum, Gas Chromatography Mass Spectroscopy (GC-MS), and Proton Nuclear Magnetic Resonance (1H NMR). TLC plate indicated the formation of our pure product due to the difference in Rf values between starting material and…

Separation through Ion Exchange Chromatography Introduction Amino acids are important molecules that serve as building blocks for all proteins that are made in every organism. Although there are millions of different proteins that can be made by all living things combined, all proteins are derived from a base of 20 amino acids that are combined together in different ways to create the diversity of life that is on Earth. Humans cannot produce all of the amino acids needed for protein synthesis,…

The variable located at the left side of the diagram, the effect of malunggay and ginger root extracts is the independent variable which in being manipulated for the dependent variable, the time spent in treating the sample infected by trichophyton rubrum. The main purpose of this study is to produce an organic antibacterial treatment for Trichophyton rubrum using Malunggay (Moringa oleifera) and Ginger (Zingiber officinale). Specifically, this study aims to answer the following: 1. In which…

To identify the unknown chemical mixture (#757), a TLC analysis was conducted using three standards (aspirin, acetaminophen, and caffeine). A TLC plate was prepared by drawing an origin line and solvent front line with a pencil. Three spot were labeled A, B, and C. A sample of each standard was spotted on the analytical TLC plate approximately 1cm from the bottom of the plate. It was then enclosed in a jar filled with 2ml of 95% ethyl acetate- 5% acetic acid. (Another laboratory group conducted…

Methods We began this experiment by laying out the needed materials to find the chromatography of Skittles Sweets and Sours and Crayola markers. Using a ruler and a pencil, a line was drawn on all thirty chromatography strips 1.5 centimeters from the bottom of the paper. This was to ensure that the starting point for each chromatography process was consistent. The chromatography of the skittles was found first. We began by pouring water into a cup. A pipette was used to transfer fifteen…

Utilizing column chromatography and recrystallization, separation and purification of the two products was obtained. The mass of 4-NBB, the para product, was 1.57 grams, while the mass of 2-NBB, the ortho product, was 0.80 grams. The para product was expected to have the higher yield, since it is more stable and consequently harder to break down throughout the experiment. Keywords Nitration, Bromobenzene, Substitution, 4-nitrobromobenzene, 2-nitrobromobenzene, Column Chromatography, Infrared…

Isolation of Chlorophyll and Beta-Carotene from Plant Leaves Yokoyama Yuri B1 (A0142349E) 1. Aim: a) To separate beta-carotene and chlorophyll via column chromatography. b) To analyse the contents of the plant extract as well as the separated beta-carotene and chlorophyll via thin layer chromatography (TLC) c) To analyse the absorption peaks of both beta-carotene and chlorophyll using UV-Vis spectroscopy 2. Results: Fig 1. UV-Vis spectroscopy results for S2 (Beta-Carotene extract) Wavelength at…

TLC and HPTLC Introductions Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent…