Analysis Of Thin Layer Chromatography

Improved Essays
In lab, a sample of crude caffeine was purified using the technique of sublimation. Thin layer chromatography (TLC) was used to verify the caffeine's chemical structure and purity. Sublimation played a huge role in the purification of the crude caffeine. The process of sublimation is to take a chemical and change it from a solid phase directly into a vapor. Another reason as to why sublimation was used is because the caffeine can be separated from its contaminant due to the fact that it does not sublimate or it sublimates at a higher temperature than caffeine. We believed that since the caffeine came directly from tea leaves, that the contaminate was chlorophyll. The chlorophyll would also be the reason that the crude caffeine product had

Related Documents

  • Improved Essays

    Experiment 11: Peptides and Amino Acids The purpose of this experiment was to study the amino acid composition of artificial sweeteners and then observe simple acid and base catalyzed reactions of the amino acid and artificial sweetener aspartame. This was achieved by performing a TLC analysis using ninhydrin as an indicator to observe the composition of the amino acid as well as two of the components that make up aspartame. This is followed by the addition of an acid or base to aliquots of diet sodas to examine the resulting reactions and compare them to a known hydrolyzed aspartame sample. The most common method of synthesizing amides is through the reaction of a primary or secondary amine with a carboxylic acid derivative.…

    • 882 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Daphnia Magna Experiment

    • 607 Words
    • 3 Pages

    Furthermore, the different concentrations were prepared in separately labeled eppendorf tubes, by adding distilled water to the 50 mM of caffeine to dilute them to the appropriate concentrations chosen. The slides were then put on the cooling chamber, after small fibers of cotton wool were placed on each well. Wide-mouthed…

    • 607 Words
    • 3 Pages
    Improved Essays
  • Superior Essays

    For the experimental rule, replenish the deoxygenated fish water with caffeine fish water to investigate the influence that caffeine can have on the fish metabolism. Take out around 100 mL of deoxygenated fish water from the chamber. Dissolve 50mg of caffeine tablet within 100 mL of fish water. Make sure that the caffeine tablet is dissolved finely by stirring the solution with a rod. Add in the caffeine-treated fish water into the chamber replacing the fish water that was taken out.…

    • 1252 Words
    • 5 Pages
    Superior Essays
  • Decent Essays

    The First Source reviewed was an article from the website Education.com called Leaf Chromatography by Beth Touchette. In the Article Leaf Chromatography Beth Touchette discusses the “how to’s” on the project itself and important facts on Leaf Chromatography. First, this article explores photosynthesis and the pigments such as chlorophyll, carotenoid, and anthocyanin and explains their colors and properties. The reading also includes such advice on what to do during project like always check the filter paper every minute or so or instead use coffee filter paper for the project.…

    • 154 Words
    • 1 Pages
    Decent Essays
  • Decent Essays

    The isolate, Pseudomonas putida NCIM 5235, was found to degrade high concentrations of caffeine within 48 hours. This may have potential applications in an environment friendly and biological removal of caffeine in caffeinated food product, beverages and industrial effluents. This method can be applied in the detoxification of harmful material and is very useful for the future.…

    • 57 Words
    • 1 Pages
    Decent Essays
  • Decent Essays

    A WHIRLPOOL 5790 gas chromatograph which had a glass column was used to analyze flavor compounds. The carrier gas was 20 ml/min flow rate nitrogen. The temperature of injection and the flame ion detector was 150℃ and 200℃ respectively. The temperature of gas chromatograph gradient started at 60℃ and maintain this level for one minute. After that, it increased by 5℃ each minute and after 20 minutes reached to 160℃ and stayed at the level for two minutes.…

    • 111 Words
    • 1 Pages
    Decent Essays
  • Decent Essays

    Polarity is a characteristic of molecules that making the charges unequally distributed causing it to create a magnetic field. One way you can check if a substance is polar or not is by doing the High Performance Chromatography Lab. Using water, different percentages (5%, 20%, and 60%) of methanol and isopropyl alcohol, Kool-Aid, and a syringe with a C-18 cartridge attached. Putting the Kool-Aid through first, made the dye stay in the cartridge, and the liquid turned clear. Next, putting the methanol alcohol, from lowest to highest percentage, made the solution turn a shade of yellow, pink, and blue.…

    • 141 Words
    • 1 Pages
    Decent Essays
  • Decent Essays

    Initially, the protein extract solution was subject to ion exchange column chromatography using SP Sepharose Fast Flow (GE Healthcare, Sweden). The column (with 5 ml of stationary phase) was equilibrated with five column volumes of sodium acetate/acetic acid 10mM pH5 and 10 ml of protein extract containing bacteriocin were injected into this. After washing the column with sodium acetate/acetic acid 10 mM pH5 (Buffer A), elution was performed using a linear gradient from sodium acetate/acetic acid 10mM pH5 in 0 minutes to 100% of NaCl 1M in sodium acetate/acetic acid 10mM pH5 (Buffer B) in 25 minutes in ÄKTA pure chromatography system (GE Healthcare), with a flow rate 1 ml/min. Protein and peptides absorbance were detected at 280 nm. 2 ml fractions were collected automatically and tested for antimicrobial activity as previously described in point…

    • 304 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    There are many legends and controversy about how and when coffee was discovered. One of the theories is that a shepherd in Ethiopia realized that after its goats ate beans from a tree, they would have so much energy that they did not sleep at night. He told the head of the monks from a monastery and this monk made a drink with the berries and found out that it had the potential to keep him up for very long hours. As the word was passed on and on, this new ideal rapidly spread around the globe. In the documentary, Cappuccino Trail, coffee is used as an example of globalization and how fair trade can be applied to this commodity.…

    • 1080 Words
    • 5 Pages
    Improved Essays
  • Decent Essays

    Conclusion Does watering plants with caffeine affect its growth? This experiment will be tested by watering nine basil plants separately, 3 with two caffeine tablets mixed with 100 milliliters of water, 3 with medium roast instant coffee mixed with 100 milliliters of water and 3 with 100 milliliters of water. Water the plants every 3 days and graph the plant growth each day. The hypothesis, caffeine will have a positive effect on the growth of plants on average, was incorrect in this experiment because, the plants watered with the caffeine tablets and instant coffee died and the plants watered with water lived. These plants were pale yellow, had wrinkled leaves and thinner stems.…

    • 296 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Gas Chromatography

    • 533 Words
    • 3 Pages

    Gas chromatography (like any other chromatography) is a separation technique in which the mobile phase is a gas. This technique is applied for the separation of volatile substances. This method separates a mixture into its components and is used to measure the concentration as well as it identifies the components. GC creates a time separation rather than physical separation which means that the components are separated in time. After they are detected, a chromatogram is generated.…

    • 533 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Caffeine Experiment

    • 1321 Words
    • 6 Pages

    The drinks we have chosen for our project is water which will be our constant, sweet tea because it is a common drink in the south, and soda, which we will be using Dr.Pepper because it was the class favorite. Sweet tea contains 0.106 mg caffeine per ml of liquid, Dr. Pepper contains 0.116 mg caffeine per ml of liquid, and water contains 0.000 mg caffeine per ml of liquid. This means that the Dr. Pepper contains the most caffeine of the three drinks we have chosen ( Sinclair and Geiger, 2000). Caffeine “is a stimulant of the central…

    • 1321 Words
    • 6 Pages
    Improved Essays
  • Great Essays

    Argumentative Essay On Caffeine

    • 1575 Words
    • 7 Pages
    • 4 Works Cited

    Caffeine is normally found in coffee, tea, sodas and chocolates. However, caffeine supplements are also available in pill form. Once caffeine is consumed it is absorbed into the body through the stomachs lining and circulated in the bloodstream. Its effects usually take place within an hour of being used but factors such as age, weight, and other drugs can alter its results. Once absorbed it becomes dispersed throughout the body mainly affecting the brain stem, which unites with the central nervous system.…

    • 1575 Words
    • 7 Pages
    • 4 Works Cited
    Great Essays
  • Great Essays

    A Separation Film Analysis

    • 1332 Words
    • 6 Pages

    A Separation is a 2011 Iranian drama, set in present-day Iran, directed by Asghar Farhadi. The movie revolves around religiosity, ethics and love. The movie is a roller-coaster of different emotions and events. It deals with different relationships in an Iranian context. The movie starts with a couple, Simin and Nader, in front of a magistrate having a divorce hearing.…

    • 1332 Words
    • 6 Pages
    Great Essays
  • Superior Essays

    To time-travel back a decade and disclose that coffee is traded in the form of individual packets alike its complementary cream or sugar would result in a roar of laughter. Nevertheless, the ability to elude the act of rinsing and preparing of coffee capsules has generated the premium level of convenience for consumers, bestowing coffee companies with tremendous success. In fact, the coffee capsule market has unimaginably expanded to the extent where environmental concerns regarding the packaging has arisen. Environmentalists and governments have contemplated and experimented with a diverse of methods to counteract the supplementary influx of unrecyclable plastic into our landfills. BBC News reported two resolutions to the obstacle in the form of restriction and reform.…

    • 783 Words
    • 4 Pages
    Superior Essays