Thin layer chromatography

pigments. Mark, on the other hand, believed that the same pigments were also located in the green portion even though they were not visible. In order to determine who was right, two tests were conducted. The first tested conducted was the paper chromatography test. In this test, samples of both the green portion of the leaf and stem of a beet were turned into liquid samples. Portions of the both samples were then added…

Slime is made by mixing together borax, glue, and water. Depending on the amount of borax added to the mixture will determine the density of the slime. For our experiment in class the independent variable was the borax. The dependent variable was slime. The control group was glue. It was important to keep the amount of glue, borax, and water constant throughout the experiment. In an experiment conducted in class it was hypothesized that the more borax added the denser the slime will be.…

Every substance reacts with force. Whether it breaks, splashes, or holds together. The science behind this experiment is the suspension. The suspension is the mixture of two substances. Where one is finely divided and dispersed in the other(SteveSpanglerScience,2015). The purpose of this experiment is to analyze the different states of viscosity, varying on the force applies to the solution. This mixture has two different states when force is being applied. When touched slowly, it is simple to…

The role of paper chromatography is to separate various and different chemicals/mixtures to show individual components within. If there are any individual components within the mixture, then they will move up the paper at different speeds and this will make the components separate. When carrying out the process, drawing an origin line near the bottom of one of the ends on the chromatography paper will be the starting point of the mixtures that you are trying to separate when they move up the…

meso-tetraphenylporphyrin (100 mg, 0.162 mmol) dissolved in dichloromethane (100 ml, 1.57 mol) in a water-jacketed flask was irradiated with compact fluorescent lamps. The solution was then concentrated in vacuo and the product subjected to column chromatography to yield the product, aldehyde, and unreacted starting butadiene which could then be recycled. 1-Phenylbutadiene…

as separate them, through the process of thin layer chromatography. Procedure Part A- Separation of Compounds by Thin Layer Chromatography • Three 100 mL beakers and watch glasses were cleaned in preparation for this experiment. Three silica gel plates were acquired from the instructor, and to each plate a line was drawn 5 mm from the edge of the plate on both sides with a pencil, this was done carefully as to not scratch or break the plate’s gel layer. After marking the plates, three…

Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good…

The mixture was stirred, and a white precipitate of dicyclohexyl urea (DCU) was created after 15–30 min. The reaction was monitored by thin layer chromatography (TLC). After approximately 6–8 h, the precipitate was filtered and the filtrate was evaporated to dryness. The resulting residue was dissolved in acetone, filtered to additional remove any DCU, and evaporated once more. 10% sodium bicarbonate…

Part A Gas Chromatography What is it? Gas chromatography is a variation of chromatography used in analytical chemistry for separating and analysing components that can be vaporised without decomposition ("Gas Chromatography", 2016). This technique involves using a gas as the mobile phase. There are two types of gas chromatography: • Gas-solid chromatography – separates substances on the basis of their different strengths of adsorption on the solid • Gas-liquid chromatography – separates…

According to Simon (2002) the silica gel is the most widely used absorbent and it is the stationary phase that is dominant in Thin layer chromatography, particles or molecules that are non-polar travel faster at the stationary phase and the molecules that are polar spend more time at the stationary phase and therefore this means that molecules that are non-polar travel larger distance on the TLC plate in comparison to molecules that are polar since the polar molecules stick to the polar…