Thin layer chromatography

Task 3 P1 When using thin layer chromatography (TLC) to test for purity of aspirin it is a quick and simple method. Firstly when using the TLC method you need to have a TLC sheet and mark on the sheet 0.5cm up on the sheet and draw a line, make sure you don’t press too hard on the paper as this will disrupt the absorbent on the paper. You need to make sure the samples have been marked on the paper so you don’t get mixed up and leave a gap between the aspirin samples so they don’t run together…

this purpose is called chromatography. 1 There are many different types of chromatography, including gas-liquid, paper, column and so on. 1 Chromatography differentiates by allowing the differences in polarity, vapor pressure, solubility and so on of different substances to be observed. 1 In our endeavor, we will be using specific types of chromatography, termed Thin-Layer Chromatography (TLC) and column chromatography. Essentially, TLC is an adsorption aspect of chromatography that uses a polar…

A sample of this reaction mixture (100 μL) was added to dichloromethane (0.5 mL). Potassium carbonate (5 mL, 5 mmol) was added to the rest of the reaction mixture which was then placed in the sonication bath. A sample of the dichloromethane layer (100 μL) was extracted out every 8 minutes and added to dichloromethane (0.5 mL) until the 32nd minute. All the samples, along with benzaldehyde and ethyl cinnamate solutions were analysed by TLC with the eluent prepared from hexane (8 mL) and…

alleged sticky bandit. The note was sent to the Tempe CSI Crime Lab for further analysis. Blaine Benson, head of the Tempe Crime Lab, has reported that paper chromatography will be used to identify the pen the bandit used. Pens from local syrup competitors were collected as evidence to compare to the ink used in the note. Paper chromatography is a common practice used to separate the colored components in the ink. The Rf value or the retention factor is the ratio of the distance traveled by the…

Methods Column chromatography was set up using a 60 ml syringe clamped to a ring stand. The syringe was plugged using a small amount of cotton. An Erlenmeyer flask was placed underneath the column to collect any excess material. Eleven 20 ml vials were labeled numbers 1 through 11. Silica gel was measured to 42 ml using a graduated cylinder and then moved into a 150 ml beaker. A slurry was created by adding 50 ml of hexane to the beaker containing the silica gel. The slurry was stirred for…

When the ink layer on paper is too thin, it is caused by less printing pressure and less amount of ink is transferred. The central position of plate cylinder is moved to change the pressure between the plate cylinder and the blanket cylinder. Center distance between the plate…

polarity of substances using the principles of chromatography. Three substances were tested using a thin layer chromatography. The column chromatography tested a mixture of two substance which were separated as a result of the chromatography. The results for both the thin layer and column chromatography supported the hypothesis. Therefore, chromatography is an effective tool that can be used to determine relative polarities. INTRODUCTION: Chromatography is a laboratory procedure that operates…

acids.2 Chromatography is the process of separating a mixture, and has many different applications throughout the world. There are a variety of ways to conduct chromatography including column chromatography, paper chromatography, and thin layer chromatography. The particular experiment conducted and discussed is paper chromatography. This experiment will use chromatography to test and identify unknown pen inks based on tests done on known pen inks. Understanding the history of chromatography can…

Abstract In this paper CuO-NH-NH2 nanoparticle was synthesized and used to remove anionic dyes from single and binary systems. The scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), Energy-dispersive X-ray spectroscopy (EDAX) and X-ray diffraction (XRD) were used to characterize the nanoparticle. Direct Red 80 (DR80) and Direct Green 6 (DG6) were used as anionic dyes. The effect of adsorbent dosage, dye concentration and pH on dye removal was evaluated. Kinetic…

Chromatography Abstract: The purpose of this lab is to use chromatography to separate and purify organic compounds. There are many different types of chromatography but most depend on the principle of adsorption. The two important components of chromatography are the adsorbent and the eluent. Adsorbents are usually solid materials that will attract and adsorb the materials to be separated. The eluent is the solvent, which carries the materials to be separated through the adsorbent. After being…