Chromatography is a method of separation in which the components of a substance are separated and distributed between two phases, which are the mobile phases and stationary phase (1). There are multiple types of chromatograph, which includes paper chromatography, column chromatograph, retention chromatography, gas chromatography, liquid chromatography etc. Each technique has its own strengths and weaknesses, but all are useful in getting separations of different components (8). Since many…
High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and…
1833, Ada met the brilliant mathematician named Charles Babbage who she became lifelong friends with, Ada was overly intrigued with his idea about a “Analytical Engine” it was a mechanical calculating machine that it’s design predated the digital computer by over 100 years. In 1842, mathematician Louis Menebrea published a memoir explaining the “Analytical Engine” which it would be translated from French to English by Ada for Babbage., In Ada’s notes she had described how the codes could be…
Paper Chromatography Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted: Materials Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre) Paper Clips/Sellotape & Skewer Stick Water soluble black marker Water Procedure: Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the…
The objectives of this lab where to determine the empirical formula of a hydrated copper (ll) sulfate sample, practice writing detailed observations in the laboratory and practice calculating mass percent composition. Before beginning taking measurements, read through the instructions below and make a neat table in which to record all the numerical data. Using tongs put a clean crucible on the hotplate. Heat the crucible for approximately 5 minutes. Turn off the hotplate. Carefully remove the…
Introduction The use of drugs of abuse has become an increasing problem throughout the world. Drugs of abuse range from anything to illegal drugs such as cocaine, misuse of prescriptions or even nicotine. Drugs of abuse are more than just harmful to a person’s health, they also have an impact the environment, including water treatment processes. The purpose of these experiments is to identify the compounds in aquatic samples from various places throughout the world. Verifying these compounds is…
With his split from Freud in 1913, Carl Jung went on to develop his theory of Analytical Psychology that made him an important forerunner of the humanist movement in psychology. While his theory has been deemed quite unusual and difficult to understand, it is nevertheless important in the development of modern day psychology. While Freud believed that the sex instinct was virtually the primary determinant of behavior, Jung thought that the development of behavior had to consist of more than…
Chromatography is a separation method used to help identify mixtures. One common form of chromatography is gas chromatography. This type of chromatography will usually use a gas as the mobile phase, and a liquid as the stationary phase (“Gas Chromatography”). A gas chromatograph is comprised of some vital components, which include: the carrier gas, flow controller, injector port, column, column oven, detector, and recorder (“Gas Chromatography”). The purpose of this lab was for an unknown…
2.4 Optical density Yang, Gao & Jiang (2012) investigated Study on the Relationships between the Optical Density and Ink Film Thickness Based on the Offset Overprint Trapping. D=D∞ (1-e-ml) formula represents the relationship between ink thickness and ink density. By using the formal of one-color ink thickness and ink density, the second density that corresponds the first major density is transferred to the equal thickness. Then they are added together. The experiment was carried out using 128…
HPLC column can be categorized into two types • Reverse Phase • Normal Phase 3.4.1.1. Reverse Phase Chromatography Reverse Phase system has a non-polar stationary phase over which polar solvents are used as mobile phase; it is generally used for separation of polar species. 3.4.1.2. Normal phase chromatography Normal Phase system has a polar stationary phase over which non-polar solvents are used as mobile phase; it is generally used for separation of non-polar species.[38]. A detection…