Absorbance

As we got the absorbance values for the three unknown solutions and the equation from the scatter graph y=0.045x + 0.0791,we did calculations as follows. Concentration of Unknown A, y=0.410 y=0.0467x + 0.0424 0.0467x = 0.410 - 0.0424 = 0.3676 x = 0.3676/0.0467 = 7.8715…

The amount of product produced by reactions can compared by analyzing the amount transmittance and absorbance of the sample. This transmittance is how much light is passes through the sample, and absorbance is how much light is absorbed by the sample. Because this product is not opaque, it blocks some of the light passing through the sample in the spectrophotometer. Therefore, the more absorbance (and less transmittance) of the sample, the more product is being produced by the…

and hydroxide ion. crystal violet hydroxide CV+ + OH- ————> CVOH Rate=k[CV+]m [OH-]n The objective for this experiment was to analyze the absorbance of the dyed molecule crystal violet using a colorimeter and the molar absorbance of the reactant. This data was used to calculate the concentration of the the crystal violet and hydroxide ions. Using the integrated kinetics method, the rate law can be calculated for single…

Titration can be modeled by the following equation: HD2- + Mg2+ + Ca2+ MgD- + H+ + Ca2+ EDTA CaEDTA + MgEDTA + HD2-Though EDTA and AA provide information regarding water hardness, they are fundamentally different tests. AA utilizes absorbance from the change in energy of the water sample to quantify the concentration of metal ions. EDTA Titration requires an actual reaction between the water sample and the EDTA which is measured by the number of EDTA drops necessary to turn…

The activity changes as measures for the absorbance and temperatures shown in Figure 1 below. Each cuvette contains the same solution, but all were tested at different temperatures measured in Celsius. Cuvette 1 is labeled as 0 Degrees Celsius which is shown below in Figure 1. The overall change in absorbance was higher than the room temperature controlled solution. Cuvette 2 is labeled as 23 Degrees Celsius and is shown below…

Introduction This experiment looks at the effects of pH, enzyme concentration, and temperature of the catalytic rate of different enzymes including alkaline phosphatase (ALP). Some of the enzymes used are involved in human digestion while others are involved in clinical tests for certain diseases (Wilson, D’Alessio, Schmitt Lavin, Keith, & BIOL 1500 Team, 2015). The ALP enzyme is used to diagnose diseases such as bone cancer, jaundice and cirrhosis of the liver. This enzyme also catalyzes the…

exceedingly toxic hydrogen peroxide will not be removed. Consequently, causing a cell to become damaged. Here, our main objective will be to determine the absorbance rates during each manipulation. If one notices a change in the absorbance rate, then it can be determined that the hydrogen peroxide is being removed. However, it there is no change in the absorbance rate, that will signify that the hydrogen peroxide is not being eliminated properly. Now, in this research, there will be several…

important to mention that these two plasmids used to come from the purification experiment performed a few weeks ago by each of the involved students. Each solution was introduced on the absorption spectroscopy apparatus in order to measure its absorbance and values of 0.307, 0.315, and 0.317 respectively were obtained at a wavelength max of 480 nm as asked by the lab manual used for the experiment. When looking at the three values obtained it can be observed than the values of solutions 2 and…

cuvette in the spectrophotometer and record the absorbance. The absorbance for each cuvette after the blank (they will be filled with nutrient broth and E.coli) will be recorded. After that measurement, a small amount of antiseptic is applied onto the E.coli in the cuvette and, depending on the antiseptic, a certain amount of time must pass and an absorbance is recorded again. I will compare the initial absorbance for each sample with the absorbance post-application of antiseptic. The…

Namely, Azulene absorbed a much higher wavelength, 576.0nm, compared to naphthalene’s absorbance of 309.3nm. This was due to differences in molecular orbitals. Naphthalene, being a benzenoid compound, had identical electron densities for its HOMO and LUMO. Azulene instead had different HOMO and LUMO. Because the orbitals in Azulene did not…