Practical Report
(Determination of blood glucose levels and qualitative carbohydrate tests)
Introduction:
The term ‘’Carbohydrates’’ is defined as the carbon compounds which contains hydroxyl groups in large quantities (King, 2013). Carbohydrates are those organic compounds that have the empirical formula CnH2nOn, or (CH2O)n (Fromm 1997). Carbohydrates are one of the four types of organic compounds in living cells that are produces during photosynthesis and are the important source of energy in both plants and animals (Ahmed 2014). They are built from monosaccharides. These monosaccharides are the small molecule that consists of three to nine carbon atoms and differ in size and in the s configuration at …show more content…
As we got the absorbance values for the three unknown solutions and the equation from the scatter graph y=0.045x + 0.0791,we did calculations as follows.
Concentration of Unknown A, y=0.410 y=0.0467x + 0.0424
0.0467x = 0.410 - 0.0424 = 0.3676 x = 0.3676/0.0467 = 7.8715 = 7.87
Concentration of Unknown B, y = 0.240 y = 0.0467x + 0.0424
0.045x = 0.240 – 0.0791 = 0.1976 x = 0.1976/0.0467 = 4.2312 = 4.23
Concentration of Unknown C, y= 0.698 y = 0.0467x + 0.0424
0.0467x = 0.698 – 0.0424 = 0.6556 x = 0.6556/0.0467 = 14.038 = …show more content…
We noticed positive response for all the four solutions A, B, C and D as the solution was in purple colour in all the test tubes. We started our second qualitative test which is an iodine test by taking mixed carbohydrate solution in 2ml pipette in to four test tubes A, B, C and D and 3 drops of dilute iodine solution was added immediately to the four test tubes where we have seen negative reactions to the three solutions A, B, C as the solution was in yellow colour and a positive reaction to the solution D as the solution turned to blue-black colour. Later we did the benedict’s test by adding benedict solute (CuSO4) in a 2.5ml pipette and mixed with 1ml mixed carbohydrate solution. The test tube was kept on a stand in a boiling water bath for 2mins and was removed to check the reactions. We have noticed positive reactions to solutions A and B whereas a negative reaction to solutions C and D as the solution is red colour. Finally we did the seliwanoff’s test by adding 2.5ml of seliwanoff’s reagent (orcinol) with 2.5ml pipette in to all the four test tube and then we also added 5 drops of mixed carbohydrate solution. Later we have kept the test tubes on stand in a boiling water bath for 10 minutes
(Determination of blood glucose levels and qualitative carbohydrate tests)
Introduction:
The term ‘’Carbohydrates’’ is defined as the carbon compounds which contains hydroxyl groups in large quantities (King, 2013). Carbohydrates are those organic compounds that have the empirical formula CnH2nOn, or (CH2O)n (Fromm 1997). Carbohydrates are one of the four types of organic compounds in living cells that are produces during photosynthesis and are the important source of energy in both plants and animals (Ahmed 2014). They are built from monosaccharides. These monosaccharides are the small molecule that consists of three to nine carbon atoms and differ in size and in the s configuration at …show more content…
As we got the absorbance values for the three unknown solutions and the equation from the scatter graph y=0.045x + 0.0791,we did calculations as follows.
Concentration of Unknown A, y=0.410 y=0.0467x + 0.0424
0.0467x = 0.410 - 0.0424 = 0.3676 x = 0.3676/0.0467 = 7.8715 = 7.87
Concentration of Unknown B, y = 0.240 y = 0.0467x + 0.0424
0.045x = 0.240 – 0.0791 = 0.1976 x = 0.1976/0.0467 = 4.2312 = 4.23
Concentration of Unknown C, y= 0.698 y = 0.0467x + 0.0424
0.0467x = 0.698 – 0.0424 = 0.6556 x = 0.6556/0.0467 = 14.038 = …show more content…
We noticed positive response for all the four solutions A, B, C and D as the solution was in purple colour in all the test tubes. We started our second qualitative test which is an iodine test by taking mixed carbohydrate solution in 2ml pipette in to four test tubes A, B, C and D and 3 drops of dilute iodine solution was added immediately to the four test tubes where we have seen negative reactions to the three solutions A, B, C as the solution was in yellow colour and a positive reaction to the solution D as the solution turned to blue-black colour. Later we did the benedict’s test by adding benedict solute (CuSO4) in a 2.5ml pipette and mixed with 1ml mixed carbohydrate solution. The test tube was kept on a stand in a boiling water bath for 2mins and was removed to check the reactions. We have noticed positive reactions to solutions A and B whereas a negative reaction to solutions C and D as the solution is red colour. Finally we did the seliwanoff’s test by adding 2.5ml of seliwanoff’s reagent (orcinol) with 2.5ml pipette in to all the four test tube and then we also added 5 drops of mixed carbohydrate solution. Later we have kept the test tubes on stand in a boiling water bath for 10 minutes