Enzyme Peroxidase Lab Report

Good Essays
Rate of Reaction for Enzyme Peroxidase

When analyzing living organisms, it is merely impossible to overlook the role that metabolism plays. Metabolism, is a series of chemical reactions that occur in a living organisms cells. When referring to metabolism, one must also note that metabolism contains two distinct metabolic pathways, catabolic and anabolic. Catabolic pathways involve the process of breaking down molecules in order to obtain the energy needed by living organisms. Meanwhile, anabolic pathways do the complete opposite. Instead of releasing energy, anabolic pathways actually take in energy. This allows for the buildup of simple molecules into more complicated ones (Urry et al., 2014). Additionally, one must also acknowledge that
…show more content…
This enzyme can be found in many organisms such as, plants, bacteria, and fungi. Its duty is to break down hydrogen peroxide. Therefore, if there is an alteration to this enzyme that impedes it from doing so, the exceedingly toxic hydrogen peroxide will not be removed. Consequently, causing a cell to become damaged. Here, our main objective will be to determine the absorbance rates during each manipulation. If one notices a change in the absorbance rate, then it can be determined that the hydrogen peroxide is being removed. However, it there is no change in the absorbance rate, that will signify that the hydrogen peroxide is not being eliminated properly. Now, in this research, there will be several experiments that will take place. First, we will observe if a change in enzyme concentration affects the rate at which an enzyme reacts. Here, one may expect a high concentration in enzyme activity to result in an increase of enzyme reaction. Thus, leading to an increase in the absorption of the extract being used. Second, we will examine to see if a change in pH causes a change in the absorbance rate. Normally, absorption rates are higher at the pHs of around 5 to 7. So, one could expect the absorption rates to be higher at these pH levels. Following, the enzyme will be observed while its structure is out of the ordinary due to boiling. Since boiling causes a denaturation, it may be anticipated that enzyme activity and extract absorption rates will decrease. Lastly, the enzyme will be witnessed undergoing different temperatures. Naturally, the absorbance rates should be higher at the temperatures of 4℃ and 23℃. Any other temperatures that are higher than these may cause a denaturation in enzymes. Therefore, causing the enzyme activity and absorption rate to decrease (Coleman,

Related Documents

  • Decent Essays

    Cumnox Reaction Lab Report

    • 1018 Words
    • 5 Pages

    4. The optimization in calcination temperature of CuMnOx (CuMn8) precursor The optimum calcination temperature of the CuMnOx catalyst was 300oC and further increasing the calcination temperature the activity of the catalyst was decreases. There was a correlation between the surface area and the calcination temperature of the catalyst. When the calcination temperature of the CuMnOx catalyst was increased above 300oC, the surface area was decreases and its analysis by BET measurement. The crystallinity was also increased as the increasing of the calcination temperature of the precursor, therefore the diffraction peaks at the XRD analysis was more crystalline phases produced by calcination at (200 - 400oC) temperature.…

    • 1018 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    For biologist it is essential to know why enzymes are important, what their functions are, and how they are affected at different temperature. Enzymes solutions have different optimal temperature as student it’s important to learn and know which enzymes requires less temperature for the enzyme capacity of amylase to be broken down . Each and every biological reaction in the interior the human cell depend on enzymes and their power as catalyst is so fast that reactions could occur in milliseconds.. But it’s in possible to even for the reaction to take place fast without enzymes. In the absence of enzymes, biological reactions crucial to life take 2.3 billion without them there will be no life at all according to Richard Wolfenden (2008), and his extensive research.…

    • 794 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    Feed Flow Rate Essay

    • 800 Words
    • 4 Pages

    4.5: Effect of feed flow rate (Fgr) on yield. Gas oil – y1 (wt %), gasoline – y2 (wt %), light gases – y3 (wt %) and coke - y4 (wt %). Operating Conditions: Tf = 600K; Trg = 960K; Fgr = 33.9Kg/s: 5: 67.8Kg/s; Frc = 480Kg/s. 4.2.5 Effect of Catalyst flow rate (Frc) on product distribution Increase in the catalyst flow rate means the catalyst spends less time in the riser, reduction in deactivation of catalyst, catalyst average activity in the riser will be higher and the cracking reactions will be more intensive. These will lead to an increase in the conversions of gas oil and subsequent increase in the yield of the products.…

    • 800 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    Another parameter to take into consideration is pH. The optimum pH of an enzyme is the value at which the reaction rate and the activity of the enzyme is highest. Again, an increase in one parameter will increase the reaction rate. Increasing the pH increases the rate of the reaction, right up until the optimum pH, after this point, the enzyme will be denatured and the reaction rate will decline. Alkaline Phosphatase has an optimum alkaline pH, as the name suggests.…

    • 1035 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    And because of that, the change in angles caused by a change in temperature will inevitably lead to a change in the cell parameter and volume. This results in the phenomenon of thermal expansion as exemplified by Table 2 above. Furthermore, the crystal structure of FePO4 tends more and more towards the β phase as temperature increases. As for the FeO4 and PO4 tetrahedra, the change in their structures can be seen through Table 5, where the O-Fe-O bond angles decrease. Furthermore, in Table 4 (taken from J. Haines, et al.…

    • 895 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    Research Question This lab will be driven by the research question, do changes in temperature (4oC, 25oC, 37oC, 50oC and 70oC) have an effect on the reaction rate of enzyme amylase and starch? Hypothesis As the temperature increases, the enzyme will have a quicker reaction time with the starch, therefore when met with the iodine, no change will occur in the colour of the solution, showing that the starch is now absent. The enzyme amylase and starch solution in 4oC and 25oC will change the colour of the iodine to a dark blue-black in the first few minutes, showing that the reaction hasn’t been completed, however for the 50oC and 70oC trials, the amylase and starch will create little to no change in the colour of the iodine when met almost immediately…

    • 1194 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    It is considered a transition metal and found to have changes in its structure at different temperatures. Therefore, this paragraph would be split into four main parts explaining iron phosphate in high temperature, low temperature and when it is in an alpha-phase as well as beta-phase. In higher temperature, the atoms of the structure iron phosphate would move around more vigorously as temperature rises higher, therefore there would be a disorder called dynamic disorder of high symmetrical beta-phase. This structure is known to be in a beta-phase when temperature is higher. It has a structure of octahedral and having a dispose or space group of p6422.…

    • 1099 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    The tilt angle is one of the significant factor which will lead to tetrahedral distortion. Tetrahedral distortion occurs when there is a change in the angle and also the change in the length of the bonds. The tilt angle as mentioned in paragraph 2 above, is inversely correlated with temperature. Another factor which led to tetrahedral distortion is due to the change in bond length and the change in O-PO angle which defers according to temperature. This means that when the temperature reaches roughly about 980K, the Fe—O—P bridging angles will increase and the tilt angles will decrease strongly.…

    • 945 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    Increase in temperature decreases the solubility of gases in water. It also increases the respiration of organisms present in the water and increases the rate of decomposition of organic matter. The temperature of the surface water varies between, while for ground water it varies as 26.7 to 30.3. pH values were measured at the site and are tabulated in the table. According to WHO the acceptable limit lies between 6.5 and 8.5. Except at one site other pH values are below 7. pH values affect the rate of intake of metals and toxins by plant and aquatic species.…

    • 967 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    For the catechol enzyme, the optimal pH is 8 and temperature desired in order to function effectively is near near room temperature. 2. When catechol is in excess and substrate is increased, the rate of reaction increases to a maximum limit due to the inability of the enzyme to work higher than its normal rate of reaction. 3. When the catechol is the substrate in excess and the enzyme increased, the rate of reaction increases exponentially due to the increasing amount of enzymes able to catabolize more…

    • 753 Words
    • 4 Pages
    Decent Essays