Enzyme Peroxidase Lab Report

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Rate of Reaction for Enzyme Peroxidase

When analyzing living organisms, it is merely impossible to overlook the role that metabolism plays. Metabolism, is a series of chemical reactions that occur in a living organisms cells. When referring to metabolism, one must also note that metabolism contains two distinct metabolic pathways, catabolic and anabolic. Catabolic pathways involve the process of breaking down molecules in order to obtain the energy needed by living organisms. Meanwhile, anabolic pathways do the complete opposite. Instead of releasing energy, anabolic pathways actually take in energy. This allows for the buildup of simple molecules into more complicated ones (Urry et al., 2014). Additionally, one must also acknowledge that
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This enzyme can be found in many organisms such as, plants, bacteria, and fungi. Its duty is to break down hydrogen peroxide. Therefore, if there is an alteration to this enzyme that impedes it from doing so, the exceedingly toxic hydrogen peroxide will not be removed. Consequently, causing a cell to become damaged. Here, our main objective will be to determine the absorbance rates during each manipulation. If one notices a change in the absorbance rate, then it can be determined that the hydrogen peroxide is being removed. However, it there is no change in the absorbance rate, that will signify that the hydrogen peroxide is not being eliminated properly. Now, in this research, there will be several experiments that will take place. First, we will observe if a change in enzyme concentration affects the rate at which an enzyme reacts. Here, one may expect a high concentration in enzyme activity to result in an increase of enzyme reaction. Thus, leading to an increase in the absorption of the extract being used. Second, we will examine to see if a change in pH causes a change in the absorbance rate. Normally, absorption rates are higher at the pHs of around 5 to 7. So, one could expect the absorption rates to be higher at these pH levels. Following, the enzyme will be observed while its structure is out of the ordinary due to boiling. Since boiling causes a denaturation, it may be anticipated that enzyme activity and extract absorption rates will decrease. Lastly, the enzyme will be witnessed undergoing different temperatures. Naturally, the absorbance rates should be higher at the temperatures of 4℃ and 23℃. Any other temperatures that are higher than these may cause a denaturation in enzymes. Therefore, causing the enzyme activity and absorption rate to decrease (Coleman,

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