Caspase

acts to inhibit the growth of tumor cells by epigenetic regulation of HDACs, causing the regulation of the cell cycle and even apoptosis for patients with pancreatic cancer. Panobinostat, similarly, can act to arrest the cell cycle and also cause caspase dependent and independent apoptosis in patients with multiple…

Sung Noh 12/11/2014 BSCI 330 Hypermethylation in patients with Myelodysplastic Syndrome Myelodysplastic Syndrome is disease of the bone marrow in which its ability to produce viable blood cells has been compromised through mutation. In healthy bone marrow the Osteocytes that live within produce hematopoietic stem cells that mature to become different components of blood: erythrocytes, neutrophils, monocytes and platelets (Saba et. al, 2007). Osteocytes that have undergone mutations produce a…

Cancer is a deadly disease where one of the only treatments we have is chemotherapy. But what is the cause of cancer? There are many environmental factors that can play a role in the diagnosis of cancer. Obesity, age, viruses, genetic mutations, and various carcinogens can cause cancer. However, what is happening at the cellular level when cancer metastasizes throughout the body? There are many different known causes, but most of them revolve around the cells ability to stop proliferating and…

are treatment options for Huntington’s disease? Some potential treatment for the disease include neural/stem cell transplantation directly in the brain. Researchers such as Friedlander have brought up the idea that due to the role of apoptosis and caspases in neurodegenerative diseases, it is a potential target for therapeutic targets. However, other implications are that in order to properly treat Huntingtons there is a requirement to treat the process of gross neuronal cell death.…

neurodegenerative conditions [53]. In the HD, mutation of huntingtin, a cytoplasmic protein, leads to it aggregation and form inclusions in cell nucleus in the brain. These aggregations, alongside the interaction of mutated huntingtin protein with regulatory caspases, are believed to be component of the pathophysiology of HD [54]. In the case of ALS, it is the SOD that aggregates. There is evidence to suggest that the accumulation of SOD aggregate may lead to inflammation and neurotoxicity…

The SH-SY5Y neuroblastoma cell line was procured from National Centre for Cell Sciences, India and maintained in (DMEM) (Sigma Aldrich, USA) and Ham 's F12 nutrient mixture (F12) (Sigma Aldrich, USA) in 1:1 ratio. The medium was supplemented with 2 mmol L-glutamine (HiMedia, India), 10% (v/v) heat inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT) and 1% antibiotics (penicillin/streptomycin). Cells were cultured at 37 ºC in a humidified CO2 ¬incubator containing 5% CO2 and…

apoptosis in a caspase independent mechanism. Earlier studies have been shown that breast cancer cells (BCCs) in a culture can phagocyte latex beads and fluorescent Matrigel. That study has confirmed the phagocytic activity by BCCs when using yeast as a test organism (40). The results of this study were demonstrated that phagocytosis of heat-killed Baker 's yeast induces apoptosis in the highly metastatic cells (MCF-7 and ZR-75-1) and in the non-metastatic cells (HCC70) in a caspase-independent…

Muckle-Wells syndrome (MWS) is a rare genetic disorder that causes hive-like skin rashes, chills, fever, partial or total hearing loss, swollen joints, loss of kidney function, and can eventually lead to amyloidosis in some patients. Muckle-Wells syndrome is named after Thomas J. Muckle and Michael V. Wells who first described the disease in April of 1962 [1]. Muckle-Wells is in a family of diseases called Cryopyrin-Associated Autoinflammatory Syndromes (CAPS) which all are related because they…

According to Ph.D. holder Pramod Dash, “it has been estimated that nearly 2 million people suffer some degree of head injury every year in the United States alone.” The Centers for Disease Control and Prevention estimates that about 1.7 million people in the United States have a traumatic brain injury each year. Brain injury can be defined as something that isn’t hereditary, degenerative, or present at birth that causes damage to the brain. The most common types of brain injury are traumatic…

TBST for 10 min (three to five times), and 2 mL of ECL™ Prime Western Blotting Detection Reagent (Biorad) was added to detect the peroxidase present. Types of antibodies Preparation ratio Β-actin 1:2000 Bax 1:1000 p53 1:1000 Cytochrome c 1:500 Caspase 8 1:2000 Table 1. The list of antibodies…