Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done. A sterile inoculating needle was used to transfer the culture. The lip of both tubes was flamed before and after transfer. The samples were incubated at 37 °C for 48 hours. The two sets of three transfers were done on two dates, October 11th and 18th.…
Agar blocks of the stem TR-S1 endophyte were cut from the pure strain actively growing on PDA Petri dishes and transferred to 1 L Erlenmeyer flasks containing a 200 g sized recipe of Corn Grit Agar(CGA). TR-S1 endophyte was incubated at room temperature at 26-28C for 21 days. Fungal metabolites were then extracted by acetone which was then partitioned with ethyl acetate and methanol respectively. TLC analysis of the ethyl acetate fraction revealed the characteristic blue spots of polyketides…
6. Three Nutrient Agar Plate (NAP) were contaminated as follow to illustrate that there are microorganism all around us and to demonstrate the necessity for proper aseptic technique. a) The lid of the first agar plate was removed and the exposed agar was placed in the building on the laboratory rack for 3 hours. The lid was replaced and labelled as “air”. It was then incubated at room temperature. b) A second Petri plate was divided in half using a marker. Two cotton sterile swab were moisten in…
Scientists use different types of nutrient agar media to help with the study of microorganisms. The purpose of this experiment was to examine the different growths of minority organisms within a variety of media cultures. These cultures included types of selective, differential, and enriched media and were applied to split sections on the agar plate. Each of the four broth cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable…
Materials and Methods Materials used in experiment 1.1A: Nutrient agar plate Materials used in experiment 1.1B: Nutrient agar plate, Sterile cotton swab In experiment 1.1B a nutrient agar plate was opened and left in an arbitrary spot in the laboratory and left untouched for three hours, at the end of the lab it was collected and incubated at . In part B a swab/swab rinse method was preformed in order to inoculate the nutrient agar plate. A sterile cotton swab was taken out of its packaging, an…
was used to make the nutrient broth. Then 2g of agar was added to the nutrients and it was dissolved in 100 mL distilled water and used as nutrient agar. The medium was sterilized. Maintenance of Bacterial Culture Bacillus cereus and Pseudomonas aeruginosa were grown on nutrient agar plates and incubated at 370C for 24 hours. The bacterial culture was maintained at 40C and sub cultured routinely to ensure viability of the…
It is not a differential medium, but it is selective because Gram-negative organisms are inhibited by the phenylethly alcohol. Nutrient Agar was used to compare the growth quality of the organism to other mediums. Mannitol Salt Agar was another medium that was spot inoculated with the unknown. Mannitol Salt Agar is a differential and selective medium. Mannitol Salt Agar are composed of Phenol red pH indicator, mannitol, and 7.5% sodium chloride. The medium is differential because it…
The first test involved the Mannitol Salt Agar (MSA). MSA is an example of both a selective and a differential media. It selects for halotolerant bacteria and with the aid of phenol-red differentiates for the ability of a microbe to ferment mannitol or not. If a microbe has the ability to ferment mannitol, it produces acidic waste products which react with the phenol-red to yield a yellow color. If the microbe is unable to ferment mannitol, it will consume the proteins present and will produce a…
Throughout this experiment I have learned several things. One thing I learned is that you can find bacterial growth in most places. However, different types of bacteria grow in different places depending on the environmental conditions and the required factors for growth for the specific bacteria. Also, I learned is the impact an error in the procedure can have. I think there were some errors in our environmental sampling lab. Something like completely removing the lid of a petri dish can alter…
Research Question What is the effect of surface area on volume ratio over rate of diffusion of pigment regarding the agar cube, measure by percentage volume of diffusion through time? Hypothesis The experiment involves the use of agar cubes. If the cells of these cubes desire a great surface area to volume ratio in order to carry out their needs (exchanging materials with the environment, receiving oxygen, getting rid of waste products), the SA:V ratio will be larger. Consequently, the rate of…