Agar

Coliscan easygel nutrient broth was stored in the freezer. Around 24 hours prior to sampling, the broth was moved to the refrigerator to thaw. The sampling procedure follows the procedure stated in the Coliscan Easygel Instructions. When we were ready to begin sampling, the thawed broth was placed into a cooler. We started sampling at Cornerstone pond then move to the Wyoming site, then downtown Grand Rapids, then Plainfield, and then Versluis lake. The procedure for Cornerstone pond and…

My experience with Agar.io Many people around the world play video games. But one of the popular PC games right now is agar.io, which is a very addicting game. Here are some of my experiences with agar.io. Of course the first time I played agar.io, it was difficult to play because I didn’t know what to do. I kept playing until I got used just to it and that’s when I noticed that I was really into this game. I got home and I went straight to my computer and started playing agar.io, at…

Eosin methyl blue (EMB) agar and MacConkey broth w/neutral red, HiMedia Laboratories Pvt. Ltd. India for coliform; Plate count agar, HiMedia Laboratories Pvt. Ltd. India for TPC; Potato dextrose agar, HiMedia Laboratories Pvt. Ltd. India for yeast & mold testing. Methyl Red Indicator, MERCK, India; Iodin, Qualigens, India; Methyl Orange, Qualigens, India; Ferroin Indicator Sol, MERCK, India; Glycerol, Qualigens, India; Methylene Blue , Qualigens, India; Oil emersion, MERK, India; Christal…

The 5 second rule is a simple rule stating that if people drop a food and pick it up within 5 seconds, the food you dropped can still be eaten. Most people follow this rule. Did you know that even if you pick up dropped food it still might contain some bacteria? Bacteria is attracted to moist so, the more moist it is the more of a risk there is that there will be bacteria in the moist food. According to Scientific American, about 87% of the 495 people they surveyed said that they would or…

Weigh out 16.80 grams of agar nutrient powder. Then add and dissolve agar nutrient powder in conical flask containing 600 ml of the deionise water. Both these flask are autoclave and sterilise at temperature of 121 ℃ about 20 minutes at 1 atmosphere pressure. After the autoclave is done for these flask containing the agar nutrient, it will be let cool to 50 ℃. The mixture is swirl throughly to mix agar and nutrients. Then the nutrient agar are pour and fill 2/3 full roughly 25-35…

Only two of the agar plates actually developed properly, one of them was the ampicillin agar plate with no plasmid (Fig 1: top left) because there was no growth seen. This was the expected result because since ampicillin kills the bacteria, the E. coli cells should not have been able to survive in the environment…

which includes Micrococcus luteus, Citrobacter freundii, Hafnia alvei, and Staphylococcus epidermidis. Micrococcus luteus is a gram positive, coccus shaped microorganism that is nonmotile and unable to grow on glucose minimal media or simmons citrate agar (Kocur, 1972;). Citrobacter freundii is a gram negative, bacillus…

After the agar plate was divided, they were then labeled “E. Coli + fresh/dry specific spice. Each of the eight agar plates were labeled this was and then ¼ of the agar plate was labeled control. This is where the plain 7mm disc went and this acted as the control of the experiment. Before any of the discs were put on the agar plate, the agar plate was prepared by making a bacterial lawn. In order to do this, a cotton swab was…

disinfectant. Take the bottle of melted agar. Remove the cap and pass the neck through the Bunsen burner flame 2-3 times. Pour the contents into the Petri dishes carefully. Replace the lid as quickly as possible. Flame the neck again and replace the cap on the bottle. Keeping the Petri dish flat on the bench, mix the agar thoroughly by gently swirling the plate as directed by your teacher. Avoid splashing the agar on to the lid or over the edge of the dish. Allow the agar to set. This is known…

wanted to prove the microbiologist that on this planet has microbes everywhere. To understand more about ubiquity experiment, an important terms need to be explained. Agar is gelatinous- complex polysaccharide obtained from red algae. An agar plate is a Petri dish that has an agar and nutrients used to culture microorganisms. Also, agar can be expand by adding other nutrients such as glucose and other…