Agar

begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…

Wash hands and spray down or wipe your work are with disinfectant. Dry off with paper towels. Label the bottom of your agar plate (petri dish) as diagrammed in your Experimental Design (note: the bottom of the plate contains the nutrient agar, which looks like a clear/beige gelatinous material). Determine your two sources of collection for bacteria specimens. When you have decided, write them in tour data table on your lab report…

of the zones varied for each microorganism. Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and oxidase test were negative. The FTM, catalase, MSA and blood agar tests were positive. After the Acid Fast stain, the cells were blue and non-acid fast. After the Endospore stain, the cells were pink. This indicated that they were non-endospores.…

Agar “1994” elaborated on the progressive study of languacultures, by analyzing cultural values that coincide with everyday language. Religion is a normal staple in the majority of households in American society. As a child your religion is chosen for you based on your parent’s values and believes. From an adolescent age children become immersed in cultural values in the religion that they’re brought up in. This choice that is embedded in your mind, can form a number one mentality about your…

Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…

Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…

microorganisms. One of the microorganisms is gram positive which can be separate from the gram negative using the four-way streak isolation from the lab manual. The purpose of the streak isolation is to obtain single colonies on a Columbia CNA agar plate. C-CNA agar is a type of…

This was streaked for isolation on a MacConkey agar plate, and then streaked once more on a separate MacConkey agar plate. Two plates were used in an effort to ensure that isolated colonies were produced. The aseptic technique was also used, and the loop was sterilized in the bactoincinerator after each streak for the same reason. The two MacConkey agar plates were placed in the incubator (35oC) and then retrieved 24 hours later. Upon retrieval, the…

Then trypticase soy agar (TSA) was poured into into six groups of 60 Petri dishes. Sixty different agar dishes were labeled based on the antibiotic used. Then the dishes were left to dry and solidify at room temperature for hour to rid of moisture. After an hour, the dishes were placed in a refrigerator at three degrees Celsius. The agar plates were monitored every few hours to make sure that the plates did not accumulate moisture. If there was visible condensation on the agar plate, they…

Introduction Bacteriophages, informally known as phages, are a specific type of virus that only infect bacteria. Since their discovery in the early 1900s , researchers have began studying the various behaviors and interactions of phages within the environment (Poxleitner, Pope, Jacobs-Sera, Sivanathan, & Hatfull, 2016). Their name, bacteriophages, is derived from the idea that they are “bacteria eaters.” Due to their bacteria-destroying nature, they have been used in phage therapy in which they…