Agar

Table 1: Table consisting of the number of colonies present and the percentage of fungal colonies. In Table 1 both fungal and bacterial colonies refer to the number of individual groups with distinct boundaries which match the assumed physical characteristic for each group. It is important to note the large increase in the number of fungal colonies as well as the decrease of bacterial colonies in the Mycophil plate both of which account for the discrepancy between the two percentages of fungal…

bacteria is by using agars and staining. The agars that are used in this lab are eosin methylene blue agar (EMB), mannitol agar, and nutrient agar. Mannitol agar is used for identifying bacteria that fall under the Staphylococcus genus (Beaver, L et al. Mannitol Salt Agar). This agar has a very high concentration of salt which is a good environment for the growth of Staphylococcus bacteria only (Beaver, L et al. Mannitol Salt Agar). The next agar that is used is EMB. EMB is a good agar for…

Salt Agar (MSA). MSA is an example of both a selective and a differential media. It selects for halotolerant bacteria and with the aid of phenol-red differentiates for the ability of a microbe to ferment mannitol or not. If a microbe has the ability to ferment mannitol, it produces acidic waste products which react with the phenol-red to yield a yellow color. If the microbe is unable to ferment mannitol, it will consume the proteins present and will produce a pink/red color. The DNase agar is a…

rate of diffusion of pigment regarding the agar cube, measure by percentage volume of diffusion through time? Hypothesis The experiment involves the use of agar cubes. If the cells of these cubes desire a great surface area to volume ratio in order to carry out their needs (exchanging materials with the environment, receiving oxygen, getting rid of waste products), the SA:V ratio will be larger. Consequently, the rate of diffusion of pigment from the agar cube calculated by the finding the…

Therefore, the isolate was tested on EMB agar, a selective and differential media that inhibits the growth of most Gram-positive organisms and can determine if an isolate is a lactose fermenter. If the isolate were a lactose fermenter this would be indicated by a dark color change in the colonies…

Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Carbohydrate Utilization: Materials: - Phenol red lactose Broth - Phenol Red Sucrose Broth - Unknown organism # 40 - Inoculating tool Procedure: Phenol red lactose and Phenol Sucrose were cultures used to identify fermentation of carbohydrates for the unknown organism #40. Phenol red is a ph. indicator as the inverted Durham tube indicates gas production…

For the production of non-volatiles, three discs of mycelia agar plugs (6 mm diameter) obtained from edges of 7 days old culture of T.koningiopsis were inoculated in 100 ml sterilized potato dextrose broth (PDB) in 250 ml conical flasks and incubated at 25 ± 1°C on a rotary shaker at 100 rpm for 10 days. The control…

1. 4 agar plates were poured several days ahead in preparation for this experiment. They were stored in a Ziploc bag in the refrigerator until ready to use. 2. To perform this experiment are the: cultures of E. coli, S. cerevisiae, and S. epidermidis, three plastic inoculation loops, candle and lighter, three poured nutrient agar plates, a disposable cup, alcohol, paper towels, bleach, a permanent marker, gloves, mask, apron, and goggles. 3. Be sure to wash hands carefully with disinfectant…

Table of Contents: Introduction………………………………………………………………………………………………………………………..2 Aim……………………………………………………………………………………………………………………………………….2 Background Info……………………………………………………………………………………………………………………2 Hypothesis…………………………………………………………………………………………………………………………….2 Variables……………………………………………………………………………………………………………………………….2/3 • Independent…………………………………………………………………………………………………………………………..2 • Dependent……………………………………………………………………………………………………………………………..3 •…

The End Zone: Measuring Antimicrobial Effectiveness with Zones of Inhibition Introduction: A Kirby-Bauer test is a microbiological method used as a form of testing bacterial resistance, it’s also known as a Zone of Inhibition test (1). The way in which the Zone of Inhibition was determined in this experiment was by measuring the diameter of the resisted zone in 3 areas and taking an average. As numerical data was required for this it means that in this experiment the Zone of Inhibition Test was…