Agar

identify the pathogen causing disease. The goal of this lab was to be able to identify the unknown pathogen, match the patient to the bacterium, and being able to identify the disease it causes. At the beginning of class, the TA handed each student an agar plate containing some unknown bacteria, each one was numbered. Mine was number 14. The teacher then asked the students to use the techniques used in class (sterilize the inoculated loop, get a small amount of the unknown bacteria, incubate) to…

using chemistry, chefs are learning new ways to make dishes for their customers. We are using molecular gastronomy to make a dessert that looks like an egg. To make the dessert egg we are using milk, agar, vanilla yogurt, mango, sugar, calcium lactate, and sodium alginate as our ingredients. agar is a jelly-like substance that…

such as a Nutrient agar plate, MacConkey’s agar plate, Gram staining, Oxidase test, UTI ID Membrane, and Eosin Ethylene Blue Agar. Materials: Testing Unknown Bacteria #8 Materials: 1. Nutrient Agar Plate (NA) 9. Hydrogen Peroxide - 2 g/ml. MacConkey’s Agar Plate (MAC) 10. UTI filter 3. Eosin Methylene Blue Agar Plate (EMB) 11. Petri plate 4. Unknown Bacteria #8 - 12. Forceps 5. Bunsen Burner - 13" Timer 6. Inoculating loop 7. Gloves 8. Oxidase test strip Methods: 1. Nutrient Agar plate:…

The same process was repeated for the next three hand cleaners and sanitiser, into three separate agar plates. The tweezers were washed and sterilised in between each agar plate The labelled plates were placed in a designated area, upside down, over the period of three…

order to correctly perform this lab, plasmids, agar plates, Escherichia coli colonies, as well as incubation periods were used. The data that was collected was completely opposite than what the actual outcome should have been. Yes there was growth in the agar plates however there was not as much growth as predicted. Another problem that arose was the fact that the pGLO did not attach to any Escherichia coli DNA, so when under a UV light, none of the agar plates glowed, even the ones that were…

of the unknown bacteria. This was done by pouring nutrient agar into a plate, waiting for it to solidify,…

in this lab are a sterilized Agar plate, sterilized, 1 P20 Micropipette. P20 micropipette tips, 4.5 milliliters of Top Ager, 0.5 milliliters of M. smegmatis, 2 serological pipets, and 5.0 milliliters of Phage buffer. The protocol is to create agreed on the bottom of the Agar plate to look like a checkerboard. Flip it back over with a cover face in the ceiling. Extract 4.5 milliliters of Top Agar and place it into the 0.5 milliliters of M.smegmatis. Release the Top Agar. Withdrawal of the mix…

The first test was hemolytic reaction; obtaining a blood agar plate, the unknown was swabbed onto the medium and incubated. A Bacitracin test was performed by also acquiring blood agar and swabbing the unknown bacteria onto the plate. A filter paper disk containing 0.04 units of bacitracin was then placed in the middle of the streak, and the plate was incubated at 37C. The next test performed was the Bile Esculin Test. A bile esculin agar slant was inoculated with the unknown, and incubated at…

Motility Test. Due to unknown 10 being gram positive, coccus shaped, and a non-sporeformer many bacteria were eliminated. One test that was run to help in differentiating the bacteria is the motility test. The motility test is preformed by taking soft agar, and heavily inoculating it an inoculating needle. The needle will create a line where the bacteria will diffuse out of it is motile. However, unknown 10 did not exhibit any diffusion, classifying it as a non-motile…

(3). This lab uses different types of media that are both selective and differential. Mannitol Salt agar (MSA), which contains high levels of sodium chloride kills most bacteria that are not members of the halotolerant Staphylococcus genus. MSA also contains mannitol, which allows differential identification because an indicator detects acid produced by the fermentation of mannitol (4). MacConkey agar, which is media containing crystal violet and bile salts inhibits Gram positive bacteria (5).…