How Do Bacteria Grow

Improved Essays
Where Do Bacteria Grow?

I had some prior knowledge about bacteria before conducting the environmental sampling lab. I knew that bacteria could be found in many different places. Also, different types of bacteria grow in different conditions. From prior experience, I remembered that to grow bacteria in a petri dish one must follow the proper technique. I chose to test how much bacteria grows on toilet handle in the bathroom. My prediction for the experiment before it was conducted was that there would be a large amount of bacterial growth on the handle of a public bathroom toilet. This was my hypothesis because many people touch the toilet handle after using the bathroom and before washing their hands. I predicted that the most bacteria would be found on objects that were touched by many different people throughout a day. To conduct this experiment, everyone in the class got in pairs. Each pair shared a petri dish to grow bacteria in. The first thing the partners did once they got their petri dish was to turn the dish upside
…show more content…
Throughout this experiment I have learned several things. One thing I learned is that you can find bacterial growth in most places. However, different types of bacteria grow in different places depending on the environmental conditions and the required factors for growth for the specific bacteria. Also, I learned is the impact an error in the procedure can have. I think there were some errors in our environmental sampling lab. Something like completely removing the lid of a petri dish can alter the results because it can allow the air to contaminate the agar. Not properly swabbing the sample can also affect the results because you may not transfer any bacteria onto the agar. Lastly, I learned that since bacterial growth can be found almost everywhere you go it is very important to wash your hands. To remove bacteria from your hands, you should wash your hands thoroughly with soap and warm

Related Documents

  • Improved Essays

    For this experiment, unknown sample #12 was used. The first step in identifying any unknown bacterium is to grow a culture on a nutrient agar plate. This bacterium was also plated on a MAC plate, which is both selective for gram-negative bacteria and differential as well. On the NA plate, bright red colonies were present; on the MAC plate there was bacterial growth as well, but no color change was observed. This lack of color change on the MAC plate indicated that the bacteria did not ferment lactose.…

    • 1060 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Antiseptic Lab Report

    • 740 Words
    • 3 Pages

    “See if you can guess what I am now … I’m a zit. Get it?” - Bluto Background: The purpose of the antiseptic lab was to show the effectiveness of multiple antiseptics: hydrogen Peroxide, Purell hand sanitizer, Water and Iodine.…

    • 740 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    The agar contain a bacteria growth. In the end of my experiments I realize my unknown organism was Salmonella Typhimurium. Salmonella Typhimurium was originally isolated in 1880 by Karl J. Erberth. S. typhimurium is a multi-organ pathogen that inhabits the lymphatic tissues of the small intestine, liver, spleen, and bloodstream of infected humans. It is not known to infect animals and is most common in developing countries with poor sanitary systems and lack of antibiotics, putting travelers to Asia, Latin America, and Africa in a high-risk group.…

    • 562 Words
    • 3 Pages
    Improved Essays
  • Superior Essays

    Essay On Nitrate Test

    • 1176 Words
    • 5 Pages

    During the phase and process of the bacterial unknown project, vast amounts of test were performed in order to determine an unknown species of bacteria. Within the many procedures of identifying the unknown bacteria, I was first given a tube with the unknown bacteria, from which later on I started subcultures by streaking bacteria from the original tube onto a tryptic soy agar (TSA) slant. Throughout the process of identifying the unknown, new subcultures were produced in order to use the newest bacteria to conduct the tests. Thus, this process allowed the unknown bacteria to grow and maintain. Before a series of test were conducted, first a gram stain test was performed in order to determine the unknown’s gram reaction and its cell morphology.…

    • 1176 Words
    • 5 Pages
    Superior Essays
  • Improved Essays

    In order to do the streaking method, an inoculating loop was sterilized with the Bunsen burner, and put into the unknown specimen then placed onto the TSA. After the plate was streaked it was then incubated at 37 degrees Celsius for 48 hours. The second step was observing the agar after incubation and identifying two different bacteria from the TSA. The third step after observation; was taking out the two bacteria with a sterilized loop and streaking it onto two Mueller Hinton plates. This allowed the separate colonies to grow further for testing.…

    • 1057 Words
    • 5 Pages
    Improved Essays
  • Decent Essays

    Purpose: The water types I will be comparing include; city water, well water, bottled water and fountain drinking water. I will take tap water from many different faucets, some will be well water and others using city, bottled water, and samples from a water fountain to grow the bacteria. Since water is an excellent solvent is can be found almost anywhere around the world, but this makes it prone to the contamination.…

    • 258 Words
    • 2 Pages
    Decent Essays
  • Superior Essays

    Using a wire loop, a small sample of the unknown test tube was plated on a nutrient agar using the streak method . The unknown test tube was then placed in a refrigerator to slow down any continued growth. The nutrient agar was labeled “VB TR1” and was placed in an incubator for 48 hours at 37 degrees Celsius. Following this procedure, I started to do research on microorganisms that presented the same type of symptoms (diarrhea and nauseous). After 48 hours, the plate was observed.…

    • 1375 Words
    • 6 Pages
    Superior Essays
  • Improved Essays

    My hypothesis is that the more commonly used and more commonly touched items in the school will collect the most bacteria and or mold. I predict that Boys sink plug puller will be collecting the most bacteria and Small spot under Gregor’s castle because they are more commonly used and did not clean every time. I predict that Mrs. Pearce closest…

    • 612 Words
    • 3 Pages
    Improved Essays
  • Great Essays

    Unknown Bacteria Essay

    • 1069 Words
    • 5 Pages

    In order for all results to be correct, aseptic technique must be used throughout the whole lab. Aseptic technique is the collective method of working and maintaining pure cultures, which requires awareness of where microbes are (4). Aseptic technique is important to make sure that the unknown bacteria is a pure culture, because a mixed culture could prevent the correct identification. A mixed culture is one that consist of 2 or more organisms (2). If there is more than one organism in the culture, there is no way to identify which one is the unknown given.…

    • 1069 Words
    • 5 Pages
    Great Essays
  • Improved Essays

    Our petri dish already had the agar prepared, so we didn’t have to go through the process of preparing this first. We grabbed cotton swabs and tried not to grab them from the ends to not get other bacteria on it. This could have been done differently though, we should have sterilized the cotton swabs to get best results. Then got the cotton swab to get my saliva and spread it across the agar and quickly closed the petri dish. Then we also grabbed the dog’s saliva the same way.…

    • 711 Words
    • 3 Pages
    Improved Essays
  • Decent Essays

    Variables Independent variable: immunity rate Dependent variable: number of people with the disease Materials: White paper cups water 1 cup of NaOH food coloring phenolphthalein indicator Procedure: Step 1: Obtain a cup. One person, unknown to everyone except the teacher, will have a cup of NaOH, the “disease.” All others will have water in their cups. Step 2: Make a point of contact with someone at random. One…

    • 446 Words
    • 2 Pages
    Decent Essays
  • Decent Essays

    Bacteria Lab Report

    • 343 Words
    • 2 Pages

    The bacteria used in the experiment are Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, and Salmonella pneumonia. These all bacteria will be culture in the nutrient broth. The first stage is making Nutrient Broth. Weigh out 5.8 grams of nutrient broth powder.…

    • 343 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Pglo Lab Report

    • 1131 Words
    • 5 Pages

    Although there was food present for the bacteria, the ampicillin contradicted with growth and reproduction. The fact that there was no DNA plasmid had a role in the bacteria not glowing. Since there was no plasmid on the plate, the gene for green fluorescent protein was not even present, so there was no way for the bacteria to glow. The hypothesis about the -pGLO plate was correct because the bacteria ended up growing but not glowing under UV light. The bacteria had such a prominent growth rate…

    • 1131 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    The lab instructor issued out a test tube labeled with letter ‘I’, which consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding contamination followed by rapidly flaming the neck of the test tube to prevent the entry and contamination of unwanted microbes.…

    • 416 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    A given test or media may fall into multiple categories. Selective and differential tests are generally less effective at species identification, and consequently, are normally only capable of presumptive identification (3). This lab uses different types of media that are both selective and differential. Mannitol Salt agar (MSA), which contains high levels of sodium chloride kills most bacteria that are not members of the halotolerant Staphylococcus genus. MSA also contains mannitol, which allows differential identification because an indicator detects acid produced by the fermentation of mannitol (4).…

    • 1018 Words
    • 4 Pages
    Improved Essays