Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done. A sterile inoculating needle was used to transfer the culture. The lip of both tubes was flamed before and after transfer. The samples were incubated at 37 °C for 48 hours. The two sets of three transfers were done on two dates, October 11th and 18th.
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A loopful of Staphylococcus epidermidis and Escherichia coli were separately and aseptically transferred to the first centrifuge tube, which was labeled “A”. A loopful of the mixture from the first centrifuge was transferred to the second tube, which was labeled “B”. This process was repeated for the third tube, which was labeled “C”, and the fourth tube, which was labeled “D”. The inoculating loop was sterilized after and before each transfer. Two drops of the mixture in centrifuge tube “A” was transferred to plate “A” and then spread using a sterilized metal spreader. This process was repeated with “B”, “C”, and “D”. The four plates were incubated at 37 °C for 48 hours. This process was done on October 11th and repeated on October 18th. The growth was observed and recorded in Table 4.
A sterile inoculating loop was used to quadrant streak samples of Staphylococcus epidermidis and Micrococcus luteus on agar plates. This process was done on October 11th and repeated on October 18th. The plates were incubated at 37 °C for 48 hours. The resulting growth was recorded in Table 6.
A sterile inoculating loop was used to spot inoculate Escherichia coli and Staphylococcus epidermidis on a phenylethyl alcohol agar plate. The plate was incubated at 37 °C for 48 hours. The resulting growth was recorded in Table 7.
A sterile inoculating loop was used to spot inoculate E. coli, S. epidermidis, S. aureus on a mannitol salt agar plate. The plate was incubated at 37 °C for 48 hours. The resulting growth was recorded in Table
A loopful of Staphylococcus epidermidis and Escherichia coli were separately and aseptically transferred to the first centrifuge tube, which was labeled “A”. A loopful of the mixture from the first centrifuge was transferred to the second tube, which was labeled “B”. This process was repeated for the third tube, which was labeled “C”, and the fourth tube, which was labeled “D”. The inoculating loop was sterilized after and before each transfer. Two drops of the mixture in centrifuge tube “A” was transferred to plate “A” and then spread using a sterilized metal spreader. This process was repeated with “B”, “C”, and “D”. The four plates were incubated at 37 °C for 48 hours. This process was done on October 11th and repeated on October 18th. The growth was observed and recorded in Table 4.
A sterile inoculating loop was used to quadrant streak samples of Staphylococcus epidermidis and Micrococcus luteus on agar plates. This process was done on October 11th and repeated on October 18th. The plates were incubated at 37 °C for 48 hours. The resulting growth was recorded in Table 6.
A sterile inoculating loop was used to spot inoculate Escherichia coli and Staphylococcus epidermidis on a phenylethyl alcohol agar plate. The plate was incubated at 37 °C for 48 hours. The resulting growth was recorded in Table 7.
A sterile inoculating loop was used to spot inoculate E. coli, S. epidermidis, S. aureus on a mannitol salt agar plate. The plate was incubated at 37 °C for 48 hours. The resulting growth was recorded in Table