Agar

CEE 467 – ENVIRONMENTAL MICROBIOLOGY NAME: ERICK PEREIRA DANTAS DATE: SEPTEMBER 4th, 2014 PROFESSOR: DR. MORTEZA ABBASZADEGAN TA: LAUREN MCBURNETT LABORATORY #2 – BACTERIOLOGICAL EXAMINATION OF WATER: MOST PROBABLE NUMBER TEST AND MEMBRANE FILTRATION METHOD ABSTRACT: The most probable number (MPN) test and the membrane filter (MF) method have as goal to detect and estimate the presence of coliforms in a sample. Their importance in the field of environmental engineering is to support…

Once completed label the bottom of the blood agar plate (not the lid) with the details of sampling area e.g. Name, Date, Location, Sampling Area. Complete the procedure 2 more times in either a clinical or a non-clinical area. Part 2: Cross-Infection Control Part A Method: Sit at a bench with elbows on the bench, head resting upon the hands and poised directly over an open blood agar plate. Recite (with enthusiasm!) the following incantations 10 times, emphasising…

Maintenance and sub-culturing of endophytic fungi The cultures were procured from already maintained repository in laboratory. The maintenance of the cultures involved preparation of Potato dextrose agar (PDA) plates, sub culturing of cultures and long term preservation. 4.1.1 Preparation of PDA (Potato dextrose agar) plates 39g of PDA was dissolved in 1000 ml double distilled water, stirred to mix properly and was transferred into 250 ml Erlenmeyer flasks and autoclaved at 121˚C at 15 psi…

the MIC (CLSI, 2006). After the MIC determination, aliquots of 100 μL from each well that does not show any bacterial growth after incubation were streaked onto BHI agar plates followed by incubation at 37°C for 20 hours. The lowest concentration that kills 100% of the initial bacterial population showing no colonies on the BHI agar was recorded as the…

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…

picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and isolation of various strains of pathogenic bacteria. 4. Fastidious Bacteria: A microorganism with a unique…

sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient agar plate for differential the isolate colonies presence. IV. To learn how to know whether the culture is a selective or differential media. The culture bacteria that…

(GYP) agar medium (glucose- 1g, yeast extract- 0.1g, agar- 15 g, distilled water 1000mL and pH 6) containing 1 % soluble starch after 5 days incubation , the plates with fungal colony were flooded with 1% iodine and 2% potassium iodide. The appearance of clear zone surrounding the colony was considered positive for amylase enzyme. Protease activity Protease assay was performed by growing the endophytic fungi on GYP gar media amended with 1% skim milk and pH was adjusted to 6.5…

Diffusion in Agar Cubes Experiment Introduction Diffusion is a paramount process to organisms because molecules enter the body cells and waste products are removed. Diffusion is the movement of particles from regions with dense areas to regions with lower density. Diffusions rate can be determined by many factors this includes, the size, synthetic composition, concentration gradient, surface area to volume ratio. The independent variable in this experiment is the size of the agar, this means…

growth of Av. paragallinarum. Secondly, the media used in disc diffusion and broth dilution testing need to be validated. This validation process is completed by performing disc diffusion and broth dilution testing of quality control strain on candidate agar or broth. Then, the antimicrobial sensitivity of Av. paragallinarum can be tested on validated media, by both disc diffusion and dilution method. Any antibiotic resistance shown in the tests will be recorded and analysed. Finally, PCR will…