Agar

Ex: In the agar diffusion method, single bacterial species is uniformly swabbed onto a nutrient agar plate. Control agent of different concentrations is placed on paper disks. These paper discs are kept on the surface of the agar. Control agent diffuses from the paper disk into the surrounding agar. Based on the zones of inhibition size around the disks, it can be evident that an effective agent will inhibit…

the cell allowing the plasmids to enter the host cell. The solutions were later poured into six different agar plates. Three of the agar plates contained ampicillin and the other three do not contain ampicillin. The control pUC18, lux, and competent cell solutions were split between the six agar plates. A portion of each solution was poured onto its own agar plate. Half was poured onto an agar plate with…

short chains and some single cells. After noting these observations, I inoculated a blood agar plate and a Phenylethyl Alcohol agar plate with the unknown #260 mixed culture broth. The blood agar plate, shown in Figure 2, was inoculated using…

plates and TSA agar, the identity was determined from a Gram-stain of a single, isolated colony; this was confirmed by isolation of that pure colony and viewing the pure sample in a Gram-stain under a microscope yielding a blue colored cocci morphology with no Gram-negative contamination. This was followed by Gram-negative isolation by a Gram-stain showing a myriad of Gram-negative bacillus rods, with no presence of Gram-positive. Further tests…

Streptomyces and Antibiosis From our group, Group 2, nutrient agar plates inoculated with E. Coli, (Images 1.1,1.2), (Images 2.1,2.2), (Images 3.1,3.2) no antibiotic activity was detected. This could be a result of the putative colonies chosen, not being Streptomyces. All selected colonies were white, or pigmented rink, raised and rough. One (1) and three (3) were inoculated with the putative Streptomyces colonies. Plate 2 was inoculated with the control Streptomyces colony. Although it was…

the gram negative isolate in the unknown #260 broth, I observed the bacterium under the microscope, created a stock culture, and conducted a variety of biochemical tests. First, I inoculated a blood agar plate and a MacConkey (MAC) agar plate with the unknown #260 mixed culture broth. The blood agar plate, shown in Figure 1, was inoculated using the quadrant streaking method and then incubated for 24 hours at 35 °C. In the third and fourth quadrants, two different bacterial cultures were present…

amount of the bacteria to the droplet of KOH. Stir it together. Raise and lower the toothpick off the slide to observe the results. Obligately aerobic organisms will grow on top but not in the stab. Motility Agar: Obtain a motility agar tube. Using an inoculation pick stab 2/3 of the way into the agar from your unknown stock culture as straight as possible. Incubate for up to 72 hours. Then observe your culture by holding it up to a light source. Results depend on visual stance, if the growth…

………………………………………………………………………... 3.0 g Sodium chloride, NaCl ……………………………………………………………… 5.0 g Lactose ………………………………………………………………………………12.5 g Bile salts No. 3 or bile salts mixture …………………………………………………1.5 g Aniline blue …………………………………………………..................................... 0.1 g Agar (optional) ………………………………………………………………….. 15.0 g Distilled water …………………………………………………..………………….1 L pH should be 7.4±0.2 after sterilization by heating. 9.6. EC medium (MERCK) Tryptose or trypticase…

For the Casein Hydolysis test, the materials used were a skim milk agar plate and the pure culture of Neisseria sicca. For the catalase test, 3% H2O2 solution dropped onto the agar plate using pipette. A few EA & JB 4 moments of waiting…

of the bacteria to grow and glow. There were two control groups in this lab, the first plate (LB agar with -pGlo E. coli) and the second plate (LB+Amp with -pGlo E. coli). There were two control groups in this lab because both a positive and a negative control were needed to be sure that the E. coli were able to grow, and that the ampicillin antibiotic were not expired or faulty. The first plate (LB agar with -pGlo E. coli) was the positive control group because the expected results were that…