INTRODUCTION Air, water and soil constitute our natural environment but with the increasing population and the prevalent lifestyle, there is a steady strain on the air we breathe, the water we drink and the soil or land on which we live and which provides us livelihood.The Xenobiotic compounds undergo a number of processes in the environment and interact with living entities i.e., cells, organs and tissues, and cause significant impairment of physiological functions. Environmental biotechnology is developing fast. This is proved by the fact that biotechnology has a growth rate of 7 percent in the market of food and pharmaceutical applications. The advent of the industrial revolution resulted in impairment of the environment through the release
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It was used to make the nutrient broth. Then 2g of agar was added to the nutrients and it was dissolved in 100 mL distilled water and used as nutrient agar. The medium was sterilized.
Maintenance of Bacterial Culture Bacillus cereus and Pseudomonas aeruginosa were grown on nutrient agar plates and incubated at 370C for 24 hours. The bacterial culture was maintained at 40C and sub cultured routinely to ensure viability of the …show more content…
The NaCl was added to the effluent at 2.5g/L at constant applied current density (0.5 A/dm2). Effluent was continuously stirred during the treatment using a magnetic stirrer. Experiments were carried out under batch conditions for 2 hours. In electro oxidation process, the effluent was treated at different concentrations of parachlorophenol 25, 50, 75 and 100 mg/L. COD was determined periodically to know the extent of degradation of the real effluent.
Microbial degradation For biological treatment, pre electrochemical treatment effluent has been used as the organic source for bacterial degradation. Microbes such as Bacillus cereus and Pseudomonas aeruginosa were tested at different concentrations (25, 50, 75 and 100mg/L) of parachlorophenol. The total volume of batch setup was maintained under aerobic and anaerobic conditions. The sample was collected every 24 hours and it was subjected to COD analysis. This experiment was continued for 7 days. Every day the samples were collected and inoculated on nutrient agar plates and observed for bacterial growth.
Analysis of COD The sample COD were determined using the dichromatic open reflux method, strictly following the American Public Health Association (APHA)
It was used to make the nutrient broth. Then 2g of agar was added to the nutrients and it was dissolved in 100 mL distilled water and used as nutrient agar. The medium was sterilized.
Maintenance of Bacterial Culture Bacillus cereus and Pseudomonas aeruginosa were grown on nutrient agar plates and incubated at 370C for 24 hours. The bacterial culture was maintained at 40C and sub cultured routinely to ensure viability of the …show more content…
The NaCl was added to the effluent at 2.5g/L at constant applied current density (0.5 A/dm2). Effluent was continuously stirred during the treatment using a magnetic stirrer. Experiments were carried out under batch conditions for 2 hours. In electro oxidation process, the effluent was treated at different concentrations of parachlorophenol 25, 50, 75 and 100 mg/L. COD was determined periodically to know the extent of degradation of the real effluent.
Microbial degradation For biological treatment, pre electrochemical treatment effluent has been used as the organic source for bacterial degradation. Microbes such as Bacillus cereus and Pseudomonas aeruginosa were tested at different concentrations (25, 50, 75 and 100mg/L) of parachlorophenol. The total volume of batch setup was maintained under aerobic and anaerobic conditions. The sample was collected every 24 hours and it was subjected to COD analysis. This experiment was continued for 7 days. Every day the samples were collected and inoculated on nutrient agar plates and observed for bacterial growth.
Analysis of COD The sample COD were determined using the dichromatic open reflux method, strictly following the American Public Health Association (APHA)