most growth compared to the other strain of yeast.
In this experiment, we used two different sugars, sucrose and glycerol, and individually placed them into two different strains of yeast. In this experiment each of the petri dishes contained 1/3 of 143 flo ↑, 140 flo ↓, and 138 WT. However, 138 WT was not inoculated because it was not being tested. For this experiment, we inoculated two kinds of sugars on a sterile cotton swab and then swabbed two petri dishes with sucrose and two petri dishes with glycerol on the two different types of yeast strains. In addition, a different cotton swab was used for obtaining each different each sugar. The glycerol and sucrose are the two independent variables in this experiment. To see if there were any changes in biofilm yeast growth we measured the diameter of the yeast in millimeters. The diameter of the yeast culture is the dependent variable. Furthermore, there was not a control in this study because we were comparing just the two carbon sources. Moreover, the replicates in this study were that each sugar was tested in two petri dishes in the two different culture strains of yeast. This results in a total of 4 replicates. Two being inoculated with Sucrose, and another two inoculated with Glycerol. Each petri dish was under the conditions of 24 degrees Celsius for 7 days straight.
The data collected in this experiment was done by measuring the size of the two yeast strains for each types of sugar in millimeters. The…
Purpose: To investigate how Porcellio Scaber reacts in terms of orthokinesis to different humidities in their habitat.
75 slaters which range from 5-10mm in length (kept in a plastic container with holes in the lid, with dirt and bark so that they are kept in a natural environment as possible so can react naturally during experiment.)
5x petri dish set ups labelled from 50-90% (One petri dish on the bottom will contain glycerol and have a mesh/gauze hot glued over top…
colony will be transferred to a clean glass slide and will be smeared and heat-fixed. The slide will be flooded with crystal violet for 1 minute and rinsing with distilled water. Next, the slide will be flooded with iodine solution for 1 minute, and rinsed with distilled water. It is then decolorized with alcohol and will be drained, and counterstained with safranin for 30 seconds. The slide will be rinsed and excess stain will be wiped out, and then air dry. Finally, the slide will be observed…
Oral Microbiome 10: Reflection 3
One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…
liquid containing the phage is known as supernatant.The supernatant was filtered through a .22 micron filter in order to extract phages. Since bacteria and other particles tend to be bigger than .22 microns, they would not fit through the filter, thus allowing only phages through. Mycobacterium smegmatis was then added to the supernatant in the amount of .5 mL. It was allowed to incubate for 5 days. After incubating, 1.4 mL of the enriched filtrate was pipetted into a microcentrifuge tube. This…
In order to obtain accurate numbers from the now grown-out Sordaria samples, a small procedure was followed. First, a sample of fungi was taken from the border of a WT and TT section in the Petri dish. A slide was prepared by placing one drop of water on the middle of it, the Sordaria sample on top, and a slide coverslip on top of the sample. The eraser of a pencil was used to lightly press on the coverslip in order to release the asci so that they can be seen. Under a microscope, a multitude of…
In this lab, we took four separate onion bulbs, labeled them A,B,C, and D, and put them in a beaker filled with water. We recorded the growth in number of roots and their average length for days one, two, and five. Then we put Bulbs B, C, and D in beakers with different amounts of caffeine and left Bulb A in water. We then recorded the number of roots and their average length on days two, three, and four. After that, we took and onion root form Bulbs A, B, C, and D, stained them, and looked for…
effective 2 antimicrobial agents are against 2 different strains of bacteria within a cultured environment.
• Small Bottle of S.Aureus bacteria in Nutrient Broth
• Small Bottle of E.coli bacteria in Nutrient Broth
• 4 Nutrient Agar plates (2 for each bacteria)
• A P200 Gilson pipette
• A box of sterile P200 yellow tips
• Sterile spreaders
• Latex gloves
• Sterile filter disks
• Test samples of antimicrobial agents (Milton and Dettol)
• Sterile distilled water…
2.5. Morphological Identification/Evaluations of Entomopathogenic Fungi
The six isolates of fungal entomopathogens were provided by Department of Entomology, Penn State USA. Preliminary morphological identifications of fungal entomopathogens such as color of colony, mycelial characteristics and spores from all six isolates were done at microscopic level.
2.6. Molecular Characterizations of Entomopathogenic Fungi
Cultures of Metarhizium fungus were grown in nutrient broth (Bacto, Australia) in…