Plasmid

have on in-vivo treatment; many are tightly regulated to maintain cell homeostasis, and alterations to the control of these systems can cause drastic changes in resistance. The genes encoding efflux proteins can be both chromosomal and harbored on plasmids, making it possible for horizontal transmission…

Deinococcus Radiodurans, "Conan the Bacterium" D. radiodurans was discovered in 1956 by Arthur W. Anderson at the Oregon Agricultural Experiment Station in Corvallis, Oregon. Tests were performed to determine whether processed foods can be sanitized by using great amounts of gamma radiation. A can of various meats was revealed to a radiation amount which was believed to destroy all known types of lifestyle, but the various meats subsequently damaged and D. radiodurans was separated. Since then,…

For the purpose of this assignment, I researched an article titled, “The Role of DJ-1 in the Pathogenesis of Endometriosis” by Priyanka Rai and Sisinthy Shivajj. The article discusses many components in which are relevant to the disease, endometriosis. Endometriosis is a disease among women in which causes infertility and severe pelvic pain along with other health concerns. It is seen to occur within 10% of woman whom are of a reproductive or sexually-active age. It is due to the occurrence…

Y. pestis is a nonmotile, gram-negative, nonsporulating, rod-shaped coccobacillus and a facultative anaerobe. Infamous for being the causative agent in plague, has been implicated in at least three devastating pandemics in modern history. The second plague pandemic includes the Black Death epidemic which caused the deaths of a third of the estimated European population in the fourteenth century (Haensch et al, 2010). More than five hundred years after the Black Death, during the third plague…

“In the 1980s pharmaceutical manufacturers, thinking infectious diseases were essentially conquered, cut back severely on searching for additional antibiotics” (Levy). Unknowingly, people all over the world are promoting the reproduction and spread of multiple-drug resistant bacteria and viruses. Every continent in the world is connected by easily accessible travel which makes infection effortless. There are numerous amounts of methods can slow down this process and keep people healthy. If the…

mmol/l, SD) and the WT diet induced a substantial increase in the pre-gene transfer cholesterol levels (mean cholesterol 38.6 ± 7.4 mmol/l, SD) (Table 1). Gene transfers with the T2/mLDLR and T2/mVLDLR vectors (in combination with SB100x, or as cis plasmids) to LDLR ko. mice resulted in a trend towards a cholesterol-lowering and stabilizing effect in the treated groups (Fig. 3A). One week after the gene transfers, all the T2/mLDLR and T2/mVLDLR treated mice displayed lowered cholesterol (change…

Introduction 1.1. Serratia Marcescens Serratia marcescens is a motile, rod-shaped, gram-negative anaerobic bacillus, that is a member of the genus Serratia, which belongs to the Enterobacteriaceae family. It has become an important opportunistic pathogen associated with a number of life-threatening diseases and nosocomial infections (Hejazi and Falkiner, 1997). S. marcescens is known to have high survival capability under hostile conditions; it is commonly found in a number of disinfectants…

Saibhuvaneswari RA1611014010048 JUMPING GENES Transposable elements (TEs), also known as "jumping genes" or transposons, are sequences of DNA that move (or jump) from one location in the genome to another. A transposable element (TE or transposon) is a DNA sequence that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic identity and genome size. Transposition often results in duplication of the TE. The transposable elements make…

release of immunoglobulins. There is limited data regarding expression of a whole immunoglobulin G. Dimeric scFV-Fc antibodies in P. pastoris attained expression levels of 10-30mg/L. YEAST EXPRESSION SYSTEM FOR THE PRODUCTION OF INTERFERON Plasmid containing cDNA of human interferon gamma is kept under the control of 3-phosphoglycerte kinase promoter in the yeast Saccharomyces cerevisiae. The amount of of expression was greater under PGK promoter than just the IFN-γ mRNA. 25 x 10 (6) units…

A. fischeriThe overall purpose of this study was to create a genomic library of Aliivibrio fischeri (A. fischeri) thus aiding in creating a restriction map of the lux operon. It also employs typical molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram…