Liquid chromatography-mass spectrometry

The main active components of MOR and COD in plasma and urine were quantified by a validated high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method. The PK parameters of each component was obtained and compared after different oral dosages. Multiple-dose studies were performed to explore the drug accumulation, and urine drugs were also analyzed to evaluate…

poisoning, fatal or otherwise. In ancient times the tribal and primitive men used to paint the arrow heads to render the more effective and killing their enemies, men and animals. Though it is very hard to define “poison” but it is a substance (solid, liquid, gas) which if introduced to the living body or brought in contact with any part there of produces ill health or death or both, by its’ constitutional or local effects or both. Again many substances which are harmless and is essential for…

Natural products either as pure compounds or as standardized extracts provide unlimited chances for new drug discoveries because of the unmatched availability of chemical diversity (Cos et al., 2006). According to the World Health Organization (WHO), more than 80% of the world's population trusts on traditional medicine for their primary healthcare needs. Plants used for traditional medicine contain a varied range of compounds that can be used to treat chronic as well as infectious diseases…

The second step of forming the amide also required TLC, and was done using the same reagents, ethyl acetate and water. The spots, however, on the plate could not be differentiated visually, and thus the reaction may not have run to completion. Mass spectrometry was used instead, and it was determined that the reaction had failed by producing a…

with Dr. Tilla S. Worgall and/or in the Laboratory of Toxicology and HPLC of the Department of Clinical Laboratories, Pontificia Universidad Católica de Chile. Sphingolipid levels will be quantified by high performance liquid chromatography-triple quadrupole-tandem mass spectrometry (HPLC-MS/MS) on an Agilent 1200 HPLC system, equipped with an Agilent C18 column as described in protocol implemented and validated by our group2. Sphingolipid profiles will include the following molecular species:…

INTRODUCTION OF PUBLIC HEALTH CONCERN A designer drug is an analog of an illegal drug that has been modified structurally and/or functionally such that it can be sold in the US because the chemical structure of the substance no longer matches a known controlled substance (Wohlfarth 2010). People, especially teens, can purchase these “new psychoactive substances” (NPS) online and from friends, without an understanding of what they are actually taking. Deaths due to designer drugs happen, and…

Several methods have been described in the literature for the determination of MNT in its pharmaceutical dosage forms or in biological samples. Examples of these methods in biological samples are gas chromatography – mass spectrometry (GC-MS) [18] in plasma and urine of rats and human while GC-FID [19] was used for MNT analysis in human urine. Also, MNT in mixture with other drugs have been assayed in human plasma using GC-FID [20]. In pharmaceutical preparations…

with trypsin overnight forming peptides. The peptides were extracted with 5% acetic acid in H2O and 5% acetic acid in CH3CN/ H2O at a 1:1 ratio, dried and stored at -200C for further analysis. B) Protein Identification A high performance liquid chromatography separation was used in presence of homemade trapping column and separation column. Both the column were packed with ReproSil-Pur C18-AQ resin. The determined peptide mixture was loaded into the trapping column with a solvent mixture of…

property. With two undergraduate students that I am training, we purified the rice wine and the fermentation product (acquired using the fermentation starter used at the winery) and performed thin-layer chromatography, high-performance liquid chromatography, and gas chromatography-mass spectrometry to calculate the concentration of farnesol. Future experiments are planned to isolate the farnesol using monoclonal antibody and to perform tissue culture to verify the effects of farnesol on cellular…

Introduction: LC-MS is used for the bioanalysis of small molecules drugs, which has molecular mass of less than 800 Da. Now a days, it is also frequently used for the bioanalytical determination of biopharmaceuticals.1 In pharmaceutical R & D, it takes a year to develop a new marketing drug identified from thousands of new compounds. The drug discovery is divided into early and late phase, during which pharmacokinetics study or bioanalysis of a drug is done. Once, the bioanalysis of a drug is…