Gram-positive bacteria

to drawing an educated conclusion. After performing the gram staining and observing which unknown culture was gram positive, a catalase test was performed. The catalase test allows lab workers to differentiate Staphylococci and Streptococci genus. The test was performed only on the gram-positive culture. Bacteria that respire by the use of oxygen, otherwise known as aerobic respiration, produce enzyme or “catalase”. This protects the bacteria from the harmful by-product produced from oxygen…

irregularly high incidence of listeriosis cases (“Listeriosis outbreak timeline”, 2009), the disease associated with infection by Listeria. This concern was disregarded until people in a nursing home fell ill and sandwich meat from the same place tested positive for the presence of Listeria monocytogenes (“Listeriosis outbreak timeline”, 2009). On August 12, 2008, the Canadian Food Inspection Agency launched an investigation of the Maple Leaf company and eventually all meat-products produced by…

phylogenetic data for the four microorganisms which includes Micrococcus luteus, Citrobacter freundii, Hafnia alvei, and Staphylococcus epidermidis. Micrococcus luteus is a gram positive, coccus shaped microorganism that is nonmotile and unable to grow on glucose minimal media or simmons citrate agar (Kocur, 1972;). Citrobacter freundii is a gram negative, bacillus…

are more effective in grams plus bacteria than gram bacteria. Also there should be no anti-microorganism activity consisting of alkyl chains that extend beyond 20 carbons. If the alkyl chain expands more than 20 carbons then it may not penetrate the memory. This would affect the memory significantly if the cell membrane could not penetrate the memory. This would cause the bacteria to be dysfunctional. When observing gram-positive bacteria one can perceive that the bacteria consist of teichoic…

fact, 1 out of every 25 hospitalized patients becomes affected by an HAI. (4) There are several types of pathogens capable of causing healthcare acquired infections. Gram-negative bacteria are known for causing infections in the bloodstream, pneumonia, wound infections, surgical site infections, and even meningitis. Gram-negative bacteria are resistant to most antibiotics which is what makes them so difficult to treat.…

2.0 Introduction Consumption of foods and beverages that encourages a healthy living, better health and lowering the risk of sickness has become very popular among the consumer whom are health conscious. Hence, a lot more attention is paid to different food extracts such as green tea extracts, grape seed extracts and ginger extracts as they posed excellent antimicrobial, antioxidant and anti-carcinogenic properties (Martin et al. 2009). These extracts have great potential in improving the…

not affect the pH of the orange juice, but it could be possible that the extracts maintained the pH of the juice and hence, limiting the growth of some microorganism. The minimum pH for the growth of yeast and mould is 1.5, whereas for the aciduric bacteria is between pH 2.9-4.5 (Aneja et al 2014). This show that the low pH environment of the juice was not low enough to eliminate the growth of…

β-mercatpoethanol (mol. wt. 78.13) from LOBA Chemie, sodium dodecyl sulfate (SDS) (mol. wt. 288.38) and urea (mol. wt. 60.06) was purchased from Merck, and ethanol was purchased from Sigma-Aldrich. Antimicrobial strain of gram negative bacteria Escherichia coli (E. coli) [MTCC 118] and gram positive bacteria Bacillus subtilis (B. subtilis) [MTCC 121] was purchased from MTCC Chandigarh, India. 2.1. Fabrication of Porous Scaffold from Keratin/Alginate binary blend: The extraction of keratin from…

Introduction Gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria. The cell wall can stain either positive or negative, depending on its chemistry.The aim of this experiment. Gram staining was the first differential technique used and still in use to make microscopic vision of bacteria clearer while also sub-divides the bacteria into two major groups: Gram-positive and Gram –negative. This experiment should help us describe how Gram staining…

consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding…