Gram-positive bacteria

Gram Negative Unknown Lab Report #20 BIOL 3444-008 Giang Nguyen TA: Malini Kotak 11/05/2014 Abstract The experiment is conducted in order to identify a randomly given unknown gram-negative microorganism. By comparative analysis of biochemical tests that were performed throughout the semester to find out its characteristics and determine the identification of bacterium out of four potential unknown was given. To figure out the characteristics, T-streak was performed to isolate…

specimen thrive in aqueous environments, therefore they are translucent and when mounted on a microscope the light passes through them making it impossible to see the specimen. Equally as important as this is that the viewer is aware of what type pf bacteria is being utilized. Both of these criteria in a lab are met with the use of a staining procedure which allows for greater contrast between the mostly translucent cells and light used by the microscope. Staining is a critical step when…

When trying to figure out the cellular morphology and arrangement the T-streak method is used to get isolated colonies from a sample of a bacteria. To begin the process a petri plate which has a layer of agar is divided into three sections in a T form. When preforming an experiment aseptic techniques are used between each step, therefore you flame the loop before and after every step. A sample is taken from a culture with an inoculated loop, then is spread in the top section of the plate in…

growing in a sterile medium, such as agar. The mixed culture can include multiple species of viruses, bacteria and parasites, which may or may not live in harmony with one another, sharing the available resources" (Kathryn Barr, 2016). I took number five test tube from the different microbes that I had the option of choosing from. From that I created a mixed culture for myself and was able to do a gram stain to narrow down my unknown microbes. A mixed culture contains many species that disperse…

journey to the microscopic world of bacteria can be likened to a child exploring the world for the first time. As the child discovers the world, she also learns several survival skills along the way. In the same manner, all the knowledge I learned from Microbiology class and laboratory sessions regarding microbial world equipped me to identify bacterial unknowns from a clinical sample. The task was to successfully separate and accurately identify two bacteria of a specimen taken from an…

this bacteria can be spread in different modes such as direct contact from an infected wound, contact with the hyaluronidase, an enzyme produced by the bacteria that destroys tissues or even skin to skin contact with an infected person. There are some difference in characteristics between Gram-positive and Gram negative bacteria, where one of most distinctive differences is that Gram-positive bacteria composes of a thick layer of peptidoglycan enveloping the plasma membrane, where as…

determine an unknown species of bacteria. Within the many procedures of identifying the unknown bacteria, I was first given a tube with the unknown bacteria, from which later on I started subcultures by streaking bacteria from the original tube onto a tryptic soy agar (TSA) slant. Throughout the process of identifying the unknown, new subcultures were produced in order to use the newest bacteria to conduct the tests. Thus, this process allowed the unknown bacteria to grow and maintain. Before a…

study was to preform many tests to isolate and identify what types of bacteria inhabiting the sample of forest soil tested. Methods: Many steps were taken to isolate and identify the different types…

biochemical tests are a good way to identify unknown bacteria from a sample. It is basically a similar to a trial and error method, but rather than using trial and error a person would be using positive and negative results. Using biochemical tests will help a person identify certain characteristics of the unknown bacteria through a series of chemical tests. Of course more than one biochemical tests must be used in order to ascertain which specific bacteria that a person may have. 2. Biochemical…

Serratia marcescens 1b. Glucose not fermented 8a. Glucose oxidized…………………………….........…………Pseudomonas aeruginosa 8b. Glucose not oxidized…………………………………………......Alcaligenes faecalis Test Question being asked Observations Interpretations Gram Statin Is the unknown gram…