Gram-positive bacteria

consists of a single dye which informs only about cell shape and size, rather than differences between cell components. Through the simple stain, it was found that the unknown is a staphylococcus microorganism which is spherical and in clusters. The gram stain was performed in laboratory six and this helped determine whether the…

Gram Staining Procedure Doctors often want to determine what type of bacteria is present in a patient who has an infection. Aside from other tests, experts use gram staining procedure as one of the techniques to differentiate bacteria according to their cell wall characteristics. It involves using a dye to see which bacteria retain either color (violet or red) in their cell walls. The importance of using the gram staining procedure is related to the choice of treatment for the patient. Here is…

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…

Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…

were performed that were specific to the gram- negative bacteria to start the identification process of the unknown. The results of the gram negative tests lead to the identification of the bacteria Alcaligenes faecalis. The gram stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria from the compound microscope which…

Proskauer Broth tubes. The tubes will be then incubated at 35°C for 48 hours. One tube will be placed with five drops of methyl red reagent, and the other with Barrit’s reagent. Methyl Red test is used for the indication of fermenting ability of bacteria, producing mixed acid. Voges-Proskauer test is used to detect the production of acetoin, also known as 2,3-butanediol. 6. Indole – Motility…

for identification. A Gram stain was done it reveal a gram positive cocci result. A streak plate was completed revealing that the microbe was round/filamentous, convex, erose, granular, yellow, and opaque. The next test was the Tryptic Soy Agar plate for color. Followed by the oxidase test and last was the Mannitol Salt Agar test. Thus microbe #1R was Sporosarcina ureae (Brady, Personal Communication). Procedure of the Gram stain Purpose: The Gram stain reveals whether…

various methods. Most stains used in microbiology are differential stain, which use more than one dye so that different cells, chemicals, or structures such as Gram staining, TTC reduction, Oxygen requirement test, Catalase test, Nitrate test, Motility, Triple Sugar test, Carbohydrate Fermentation test, and the IMViC test. Especially, the Gram stain differentiates between two large groups of microorganisms. Plan 03-05-16 The Unknown I.D. Project determines identification of a…

needed to do to get started was to do a gram stain. This would help me decide which kind of further testing would need to be done in order to help identify the organism that is causing gastrointestinal distress in Brown County. After doing a gram stain test on my unknown, I found that it was a gram negative organism. This prompted me to do some tests that are specifically for gram negative organisms. When I looked under the microscope I seen some rod shaped bacteria. So what is this organism and…

lab were used due to the fact that some tests were not listed on the manual, while others did not apply to the category of the bacteria. Test used to find the morphology characteristics of the unknown yielded that the colony of the unknown was yellow pigmented and the shape of the cell was rod. More information was found by examine the cell wall structure of the bacteria, Gram test revealed…