Gram-positive bacteria

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    Gram Film

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    Organisms present on the Gram film were obtained from patient sputum. This suggests that the organisms can be present either in the airways or in the lungs. Gram film shows Gram-positive (purple- dark colour) elongated oval shaped (cocci) cells. Bacteria are present as diplococci (pairs of cocci), nevertheless they also appeared as single cells and short chains of few cells (four cells chain is visible at the Gram film). Morphological appearance of bacteria, Gram stain and presence of the…

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    Microorganisms Lab Report

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    consists of a single dye which informs only about cell shape and size, rather than differences between cell components. Through the simple stain, it was found that the unknown is a staphylococcus microorganism which is spherical and in clusters. The gram stain was performed in laboratory six and this helped determine whether the…

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    Pedicure Spa Case Study

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    Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…

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    Mini Unknown Microbe. 1r

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    for identification. A Gram stain was done it reveal a gram positive cocci result. A streak plate was completed revealing that the microbe was round/filamentous, convex, erose, granular, yellow, and opaque. The next test was the Tryptic Soy Agar plate for color. Followed by the oxidase test and last was the Mannitol Salt Agar test. Thus microbe #1R was Sporosarcina ureae (Brady, Personal Communication). Procedure of the Gram stain Purpose: The Gram stain reveals whether…

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    Gram Draining Report

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    needed to do to get started was to do a gram stain. This would help me decide which kind of further testing would need to be done in order to help identify the organism that is causing gastrointestinal distress in Brown County. After doing a gram stain test on my unknown, I found that it was a gram negative organism. This prompted me to do some tests that are specifically for gram negative organisms. When I looked under the microscope I seen some rod shaped bacteria. So what is this organism and…

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    Bergey's Manual

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    lab were used due to the fact that some tests were not listed on the manual, while others did not apply to the category of the bacteria. Test used to find the morphology characteristics of the unknown yielded that the colony of the unknown was yellow pigmented and the shape of the cell was rod. More information was found by examine the cell wall structure of the bacteria, Gram test revealed…

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    various methods. Most stains used in microbiology are differential stain, which use more than one dye so that different cells, chemicals, or structures such as Gram staining, TTC reduction, Oxygen requirement test, Catalase test, Nitrate test, Motility, Triple Sugar test, Carbohydrate Fermentation test, and the IMViC test. Especially, the Gram stain differentiates between two large groups of microorganisms. Plan 03-05-16 The Unknown I.D. Project determines identification of a…

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    Proskauer Broth tubes. The tubes will be then incubated at 35°C for 48 hours. One tube will be placed with five drops of methyl red reagent, and the other with Barrit’s reagent. Methyl Red test is used for the indication of fermenting ability of bacteria, producing mixed acid. Voges-Proskauer test is used to detect the production of acetoin, also known as 2,3-butanediol. 6. Indole – Motility…

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    the gram reaction of the unknown, a Gram stain was done. This process differentiates between a Gram-positive and Gram-negative microorganism. This is done in four steps. The microorganism was stained with iodine, crystal violet, alcohol and acetone, and safranin. Iodine was used first as it acts as a mordant. It forms an insoluble complex by binding with the primary stain. It is then stained with crystal violet. Alcohol and acetone acts as a decolorizer. A distinct feature between both Gram…

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    TLC Semi Quantitative Dpph

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    2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…

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