first isolated by Alexander Ogston. Ogston described the bacteria, which he isolated from pus collected from surgical wounds, as micrococci (Thomer et al., 2015). This description was given because of the sphere-shape of the bacteria and the grape-like clusters that they form. After Ogston’s discovery, Friedrich Rosenbach differentiated between two staphylococci bacteria, which were isolated from humans (Thomer et al., 2015). These bacteria were named S. aureus and S. albus. This nomenclature…
between gram negative and gram positive bacterium. This is important when doing gram staining experiments. It also looks at the importance of microbiology. DIFFERENCES OF GRAM NEGATIVE AND GRAM POSITIVE BACTERIUM There are notable differences between the gram negative and gram positive bacterium. Gram negative bacterium is surrounded by a thin peptidoglycan cell wall while Gram positive has a thick layer of peptidoglycan. The peptidoglycan around the gram negative is about 8-10 mm while in gram…
The purpose of the infections caused by gram-positive cocci lab exercise is to utilize different identification and diagnostic techniques to differentiate various groups of bacteria, which cause infection. Our primary goal is first to establish the presence of an infection; then, determine which particular pathogens, or groups of pathogens, are the underlying cause of the infection. In the clinic, infections manifesting from gram-positive cocci are a common and growing problem. Two genera in…
Discussion: The isolate studied was tested for innate antibiotic function on the following media: AC, LB, and 10% TSA. The ESKAPE relative strain that presented a response to the isolate was B.subtilis. In all cases except for that of LB, the isolate showed antibiotic qualities towards B.subtilis by presenting a zone of clearance on AC and 10% TSA media. This behavior is shown in Figures 4, 5, and 6.In the case with LB, B.subtilis inhibited the growth of the isolate as depicted by the empty…
CLOSTRIDIUM TETANI Introduction Clostridium tetani is a microorganism that has effected the lives of many people. This bacteria is the cause of the disease many people have come to know as Tetanus. It infects its victims by releasing a toxin in the human body that causes an infection in the nervous system (Hassel, 2013). The first experimentations with Tetanus began in the 1800’s when scientists began infecting animals with samples from infected humans. In 1889, one scientist by the name…
inhibiting bacteria. This result however, does not signify that natural remedies are inadequate at inhibiting bacteria. It reflects that natural remedies are ineffective in inhibiting gram-positive bacteria. First natural product: Saliva for instance, is one of the body’s many defence mechanisms. Its main function is to prevent adhesion of bacteria to oral surfaces. This means that saliva does not specialise in killing bacteria. Research has indicated that saliva have anti-bacteria property…
GENTAMICIN The drug selected for study is Gentamicin. Gentamicin is an aminoglycoside antibiotic composed of a mixture of related Gentamicin components and fractions and is used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms. Isolation and Preliminary studies 2 demonstrated that it is a mixture of basic, water soluble antibiotic containing the aminocyclitol 2-deoxystreptamine and two additional amino sugars. Chromatographic separation of…
is essential (Bush 2014). One recent study shows 50% of HIV/AIDS patients had severe sepsis at ICU admission or during the ICU stay. An astounding 90% of nosocomial infections were the source of sepsis and the microbiology of infections was mostly gram-negative rods Serratia marcescens (three) made up 7% (Japiassú…
Proteus Mirabilis Proteus mirabilis is defined by the slide share website as a disease caused by microbial invasion of the genitourinary track that extends from the renal cortex of the kidney to the urethral meatus. Proteus mirabilis can be laboratory diagnosed by the specimen collection, transported and storage and laboratory methods, but the laboratory methods that would be focused on is specimen collection. It is often the first determine diagnosis and treatment which would give a more…
DISCUSSION: Pseudomonas aeruginosa was selected to study their antimicrobial activity due to the production of pyocyanin, an antimicrobial phenazine pigment. For this study different clinical specimen were collected and from these P. aeruginosa was isolated. To isolate good pyocyanin producing P. aeruginosa, repeated plating of all the isolates on cetrimide agar helped in final selection of the 5 isolates. To examine the antimicrobial activity of those isolates, in vitro inhibition by cross…